Human TCF-3/E2A Antibody

Catalog # Availability Size / Price Qty
MAB44061
MAB44061-SP
TCF-3/E2A in HeLa Human Cell Line.
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Citations (1)
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Human TCF-3/E2A Antibody Summary

Species Reactivity
Human
Specificity
Detects human TCF-3/E2A in direct ELISAs. In this format, 100% cross-reactivity with recombinant human (rh) TCF-12 is observed and no cross-reactivity with rhTCF-1, 2, 4, 7L1, 7L2 or rhLEF-1 is observed.
Source
Monoclonal Rat IgG2B Clone # 401709
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TCF-3/E2A
Gly100-Glu500
Accession # NP_003191
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry TCF-3/E2A antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

TCF-3/E2A in HeLa Human Cell Line. TCF-3/E2A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human TCF-3/E2A Monoclonal Antibody (Catalog # MAB44061) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF-3/E2A

TCF-3, also known as E2A and ITF-1, is a member of the E protein homeobox family. TCF-3 regulates checkpoints that allow B lineage commitment and B and T cell receptor gene rearrangement. Two isoforms named E12 and E47 are alternatively spliced within the DNA binding and HLH domain. The region of human TCF-3 used as the immunogen, which is identical for E12 and E47, shares 80% and 79% aa identity with mouse and rat TCF-3, respectively.

Long Name
Transcription Factor 3/E2-alpha
Entrez Gene IDs
6929 (Human); 21423 (Mouse); 312451 (Rat)
Alternate Names
bHLHb21; bHLHb21TCF-3; Class B basic helix-loop-helix protein 21; E2A immunoglobulin enhancer-binding factor E12/E47; E2A; E2AKappa-E2-binding factor; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; ITF1Transcription factor ITF-1; Kappa-E2-binding factor; MGC129647; MGC129648; Pan2; TCF-3; Tcfe2a; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor 3; transcription factor E2-alpha; VDIR; VDR interacting repressor

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Citation for Human TCF-3/E2A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. MicroRNA-449a maintains self-renewal in liver cancer stem-like cells by targeting Tcf3
    Authors: Q Zhang, Z Yang, J Shan, L Liu, C Liu, J Shen, X Chen, Y Xu, J Chen, Q Ma, L Yang, C Qian
    Oncotarget, 2017-11-27;8(66):110187-110200.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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