Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all TEX19 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TEX19
Met1-Ser164
Accession # Q8NA77

Specificity

Detects human TEX19 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human TEX11 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human TEX19 Antibody

Detection of Human TEX19 antibody by Western Blot.

Detection of Human TEX19 by Western Blot.

Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Human TEX19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6319) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TEX19 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

TEX19 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

TEX19 in BG01V Human Stem Cells.

TEX19 was detected in immersion fixed BG01V human embryonic stem cells using Human TEX19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6319) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human TEX19 Antibody

Application
Recommended Usage

Immunocytochemistry

10 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Western Blot

0.5 µg/mL
Sample: NTera‑2 human testicular embryonic carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TEX19

TEX19 (testis expressed sequence 19) is an 18 kDa (predicted) protein with restricted cell expression. It is expressed in spermatogonia as well as pluripotent stem cells. TEX19 has been found in both nucleus and cytoplasm. Functionally, TEX19 is proposed to guard against inappropriately activated endogenous retroviruses during meiosis, and thus prevent deleterious mutations. Human TEX19 is 164 amino acids (aa) in length. There is no identifiable structural module within the protein. There are, however, two sequences that are conserved in TEX19 from multiple species. These are an MCP domain between aa 1-58, and a VPTEL domain that spans aa 120-157. Over aa 1-164, human TEX19 shares only 53% aa identity with a highly divergent and substantially larger 42 kDa mouse TEX19.1 protein.

Long Name

Testis Expressed 19

Alternate Names

FLJ35767

Entrez Gene IDs

400629 (Human); 70956 (Mouse); 691099 (Rat)

Gene Symbol

TEX19

UniProt

Q8NA77 (Human)

Additional TEX19 Products

Product Documents for Human TEX19 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TEX19 Antibody

For research use only

Related Research Areas

Primordial and Mature Germ Cells

Citations for Human TEX19 Antibody

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Protocols

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