Human TGF-alpha Quantikine ELISA Kit

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Human TGF-alpha ELISA Standard Curve
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Citations (27)
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Human TGF-alpha Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Human Milk (50 uL)
7.1 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk)
Natural and recombinant human TGF-alpha
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human TGF-alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TGF-alpha in cell culture supernates, serum, plasma, and human milk. It contains E. coli-expressed recombinant human TGF-alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TGF-alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TGF-alpha.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 91.7 215 419 93.1 221 415
Standard Deviation 3.8 9.6 11.1 6.3 10.7 18.1
CV% 4.1 4.5 2.6 6.8 4.8 4.4


The recovery of TGF-alpha spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 92-108
EDTA Plasma (n=4) 99 88-114
Heparin Plasma (n=4) 103 88-114
Serum (n=4) 103 96-114


To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TGF-alpha were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TGF-alpha ELISA Linearity

Scientific Data

Human TGF-alpha ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-alpha

TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors with soluble forms released by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and plays a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. TGF-alpha binds and activates the EGF receptor. It induces fibroblast proliferation, epithelial development, keratinocyte migration, and angiogenesis.

Long Name:
Transforming Growth Factor alpha
Entrez Gene IDs:
7039 (Human)
Alternate Names:
protransforming growth factor alpha; TFGA; TGFA; TGFalpha; TGF-alpha; transforming growth factor, alpha; transforming growth factor-alpha
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human TGF-alpha Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Unraveling the differential impact of PAHs and dioxin-like compounds on AKR1C3 reveals the EGFR extracellular domain as a critical determinant of the AHR response
    Authors: C Vogeley, NC Sondermann, S Woeste, AA Momin, V Gilardino, F Hartung, M Heinen, SK Maa beta, M Mescher, M Pollet, KM Rolfes, CFA Vogel, A Rossi, D Lang, ST Arold, M Nakamura, T Haarmann-S
    Environment international, 2022;158(0):106989.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Plateletcrit and Mean Platelet Volume in the Evaluation of Alcoholic Liver Cirrhosis and Nonalcoholic Fatty Liver Disease Patients
    Authors: A Michalak, H Cicho?-Lac, M Guz, J Kozicka, M Cybulski, W Jeleniewic
    BioMed Research International, 2021;2021(0):8867985.
    Species: Human
    Sample Types: Serum
  3. NRF2 Enables EGFR Signaling in Melanoma Cells
    Authors: JKC Kre beta, C Jessen, A Marquardt, A Hufnagel, S Meierjohan
    International Journal of Molecular Sciences, 2021;22(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. TGFalpha Promotes Chemoresistance of Malignant Pleural Mesothelioma
    Authors: B Staumont, M Jamakhani, C Costa, F Vandermeer, SN Sriramared, G Redouté, C Mascaux, P Delvenne, P Hubert, R Safari, L Willems
    Cancers (Basel), 2020;12(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Anti-epidermal growth factor vaccine antibodies increase the antitumor activity of kinase inhibitors in ALK and RET rearranged lung cancer cells
    Authors: J Codony-Ser, S García-Rom, MÁ Molina-Vil, J Bertran-Al, S Viteri, E d'Hondt, R Rosell
    Transl Oncol, 2020;14(1):100887.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Towards an evaluation of alcoholic liver cirrhosis and nonalcoholic fatty liver disease patients with hematological scales
    Authors: A Michalak, H Cicho?-Lac, M Guz, J Kozicka, M Cybulski, W Jeleniewic, A Stepulak
    World Journal of Gastroenterology, 2020;26(47):7538-7549.
    Species: Human
    Sample Types: Serum
  7. Combinations of Bevacizumab and Erlotinib show activity in colorectal cancer independent of RAS status
    Authors: P Mésange, A Bouygues, N Ferrand, M Sabbah, AE Escargueil, A Savina, B Chibaudel, C Tournigand, T Andre, A de Gramont, AK Larsen
    Clin. Cancer Res., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  8. ErbB activation signatures as potential biomarkers for anti-ErbB3 treatment in HNSCC
    Authors: D Alvarado, GF Ligon, JS Lillquist, SB Seibel, G Wallweber, VM Neumeister, DL Rimm, G McMahon, TM LaVallee
    PLoS ONE, 2017;12(7):e0181356.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. DUOX-Mediated Signaling Is Not Required for LPS-Induced Neutrophilic Response in the Airways.
    Authors: Chang S, Linderholm A, Harper R
    PLoS ONE, 2015;10(7):e0131810.
    Species: Mouse
    Sample Types: BALF
  10. Circulating hepatocyte growth factor is correlated with resistance to cetuximab in metastatic colorectal cancer.
    Authors: Yonesaka, Kimio, Satoh, Taroh, Ueda, Shinya, Yoshida, Takeshi, Takeda, Masayuki, Shimizu, Toshio, Okamoto, Isamu, Nishio, Kazuto, Tamura, Takao, Nakagawa, Kazuhiko
    Anticancer Res, 2015;35(3):1683-9.
    Species: Human
    Sample Types: Plasma
  11. Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype.
    Authors: Zhang Z, Dong Z, Lauxen I, Filho M, Nor J
    Cancer Res, 2014;74(10):2869-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Activation of HER family signaling as a mechanism of acquired resistance to ALK inhibitors in EML4-ALK-positive non-small cell lung cancer.
    Authors: Tanizaki J, Okamoto I, Okabe T, Sakai K, Tanaka K, Hayashi H, Kaneda H, Takezawa K, Kuwata K, Yamaguchi H, Hatashita E, Nishio K, Nakagawa K
    Clin Cancer Res, 2012;18(22):6219-26.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. ETS-1 oncogenic activity mediated by transforming growth factor alpha.
    Authors: Holterman CE, Franovic A, Payette J
    Cancer Res., 2010;70(2):730-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer.
    Authors: Han SW, Oh DY, Im SA, Park SR, Lee KW, Song HS, Lee NS, Lee KH, Choi IS, Lee MH, Kim MA, Kim WH, Bang YJ, Kim TY
    Br. J. Cancer, 2009;100(2):298-304.
    Species: Human
    Sample Types: Serum
  15. Cross-talk between estrogen receptor and epidermal growth factor receptor in head and neck squamous cell carcinoma.
    Authors: Egloff AM, Rothstein ME, Seethala R, Siegfried JM, Grandis JR, Stabile LP
    Clin. Cancer Res., 2009;15(21):6529-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Molecular response to cetuximab and efficacy of preoperative cetuximab-based chemoradiation in rectal cancer.
    Authors: Debucquoy A, Haustermans K, Daemen A, Aydin S, Libbrecht L, Gevaert O, De Moor B, Tejpar S, McBride WH, Penninckx F, Scalliet P, Stroh C, Vlassak S, Sempoux C, Machiels JP
    J. Clin. Oncol., 2009;27(17):2751-7.
    Species: Human
    Sample Types: Plasma
  17. RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects.
    Authors: Kreeger PK, Mandhana R, Alford SK, Haigis KM, Lauffenburger DA
    Cancer Res., 2009;69(20):8191-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Epidermal Growth Factor Receptor Blockade in Combination with Conventional Chemotherapy Inhibits Soft Tissue Sarcoma Cell Growth In vitro and In vivo.
    Authors: Ren W, Korchin B, Zhu QS, Wei C, Dicker A, Heymach J, Lazar A, Pollock RE, Lev D
    Clin. Cancer Res., 2008;14(9):2785-2795.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Modification of the primary tumor microenvironment by transforming growth factor alpha-epidermal growth factor receptor signaling promotes metastasis in an orthotopic colon cancer model.
    Authors: Sasaki T, Nakamura T, Rebhun RB, Cheng H, Hale KS, Tsan RZ, Fidler IJ, Langley RR
    Am. J. Pathol., 2008;173(1):205-16.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Activation of the epidermal growth factor receptor (EGFR) by a novel metalloprotease pathway.
    Authors: Bergin DA, Greene CM, Sterchi EE, Kenna C, Geraghty P, Belaaouaj A, Taggart CC, O'Neill SJ, McElvaney NG
    J. Biol. Chem., 2008;283(46):31736-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Effective inhibition of the epidermal growth factor/epidermal growth factor receptor binding by anti-epidermal growth factor antibodies is related to better survival in advanced non-small-cell lung cancer patients treated with the epidermal growth factor cancer vaccine.
    Authors: Garcia B, Neninger E, de la Torre A, Leonard I, Martinez R, Viada C, Gonzalez G, Mazorra Z, Lage A, Crombet T
    Clin. Cancer Res., 2008;14(3):840-6.
    Species: Human
    Sample Types: Serum
  22. MMP-12 induces IL-8/CXCL8 secretion through EGFR and ERK1/2 activation in epithelial cells.
    Authors: Le Quement C, Guenon I, Gillon JY, Lagente V, Boichot E
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(6):L1076-84.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Alteration of the serum levels of the epidermal growth factor receptor and its ligands in patients with non-small cell lung cancer and head and neck carcinoma.
    Authors: Lemos-Gonzalez Y, Rodriguez-Berrocal FJ, Cordero OJ, Gomez C, Paez de la Cadena M
    Br. J. Cancer, 2007;96(10):1569-78.
    Species: Human
    Sample Types: Serum
  24. EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts: implications for osteolytic bone metastases.
    Authors: Zhu J, Jia X, Xiao G, Kang Y, Partridge NC, Qin L
    J. Biol. Chem., 2007;282(37):26656-64.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. The response of human epithelial cells to TNF involves an inducible autocrine cascade.
    Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
    Cell, 2006;124(6):1225-39.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Mek1 alters epidermal growth and differentiation.
    Authors: Scholl FA, Dumesic PA
    Cancer Res., 2004;64(17):6035-40.
    Species: Porcine
    Sample Types: Cell Culture Supernates


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