Human TGF-alpha Quantikine ELISA Kit

(18 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Human Milk (50 uL)
  • Sensitivity
    7.1 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk)
  • Specificity
    Natural and recombinant human TGF-alpha
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human TGF-alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TGF-alpha in cell culture supernates, serum, plasma, and human milk. It contains E. coli-expressed recombinant human TGF-alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TGF-alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TGF-alpha.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk
Intra-Assay Precision Inter-Assay Precision
Standard Deviation3.89.611.16.310.718.1


The recovery of TGF-alpha spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 92-108
EDTA Plasma (n=4) 99 88-114
Heparin Plasma (n=4) 103 88-114
Serum (n=4) 103 96-114
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TGF-alpha were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TGF-alpha Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TGF-alpha
TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors with soluble forms released by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and plays a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. TGF-alpha binds and activates the EGF receptor. It induces fibroblast proliferation, epithelial development, keratinocyte migration, and angiogenesis.
    • Long Name
      Transforming Growth Factor alpha
    • Entrez Gene IDs
      7039 (Human);
    • Alternate Names
      protransforming growth factor alpha; TFGA; TGFA; TGFalpha; transforming growth factor, alpha; transforming growth factor-alpha;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 18
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    Sample Type
    1. DUOX-Mediated Signaling Is Not Required for LPS-Induced Neutrophilic Response in the Airways.
      Authors: Chang S, Linderholm A, Harper R
      PLoS ONE, 2015;10(7):e0131810.
      Species: Mouse
      Sample Type: BALF
    2. Circulating hepatocyte growth factor is correlated with resistance to cetuximab in metastatic colorectal cancer.
      Authors: Yonesaka, Kimio, Satoh, Taroh, Ueda, Shinya, Yoshida, Takeshi, Takeda, Masayuki, Shimizu, Toshio, Okamoto, Isamu, Nishio, Kazuto, Tamura, Takao, Nakagawa, Kazuhiko
      Anticancer Res, 2015;35(3):1683-9.
      Species: Human
      Sample Type: Plasma
    3. Activation of HER family signaling as a mechanism of acquired resistance to ALK inhibitors in EML4-ALK-positive non-small cell lung cancer.
      Authors: Tanizaki J, Okamoto I, Okabe T, Sakai K, Tanaka K, Hayashi H, Kaneda H, Takezawa K, Kuwata K, Yamaguchi H, Hatashita E, Nishio K, Nakagawa K
      Clin Cancer Res, 2012;18(22):6219-26.
      Species: Human
      Sample Type: cell culture supernate
    4. ETS-1 oncogenic activity mediated by transforming growth factor alpha.
      Authors: Holterman CE, Franovic A, Payette J
      Cancer Res., 2010;70(2):730-40.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Molecular response to cetuximab and efficacy of preoperative cetuximab-based chemoradiation in rectal cancer.
      Authors: Debucquoy A, Haustermans K, Daemen A, Aydin S, Libbrecht L, Gevaert O, De Moor B, Tejpar S, McBride WH, Penninckx F, Scalliet P, Stroh C, Vlassak S, Sempoux C, Machiels JP
      J. Clin. Oncol., 2009;27(17):2751-7.
      Species: Human
      Sample Type: Plasma
    6. RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects.
      Authors: Kreeger PK, Mandhana R, Alford SK, Haigis KM, Lauffenburger DA
      Cancer Res., 2009;69(20):8191-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer.
      Authors: Han SW, Oh DY, Im SA, Park SR, Lee KW, Song HS, Lee NS, Lee KH, Choi IS, Lee MH, Kim MA, Kim WH, Bang YJ, Kim TY
      Br. J. Cancer, 2009;100(2):298-304.
      Species: Human
      Sample Type: Serum
    8. Cross-talk between estrogen receptor and epidermal growth factor receptor in head and neck squamous cell carcinoma.
      Authors: Egloff AM, Rothstein ME, Seethala R, Siegfried JM, Grandis JR, Stabile LP
      Clin. Cancer Res., 2009;15(21):6529-40.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. MMP-12 induces IL-8/CXCL8 secretion through EGFR and ERK1/2 activation in epithelial cells.
      Authors: Le Quement C, Guenon I, Gillon JY, Lagente V, Boichot E
      Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(6):L1076-84.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Effective inhibition of the epidermal growth factor/epidermal growth factor receptor binding by anti-epidermal growth factor antibodies is related to better survival in advanced non-small-cell lung cancer patients treated with the epidermal growth factor cancer vaccine.
      Authors: Garcia B, Neninger E, de la Torre A, Leonard I, Martinez R, Viada C, Gonzalez G, Mazorra Z, Lage A, Crombet T
      Clin. Cancer Res., 2008;14(3):840-6.
      Species: Human
      Sample Type: Serum
    11. Activation of the epidermal growth factor receptor (EGFR) by a novel metalloprotease pathway.
      Authors: Bergin DA, Greene CM, Sterchi EE, Kenna C, Geraghty P, Belaaouaj A, Taggart CC, O'Neill SJ, McElvaney NG
      J. Biol. Chem., 2008;283(46):31736-44.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Epidermal Growth Factor Receptor Blockade in Combination with Conventional Chemotherapy Inhibits Soft Tissue Sarcoma Cell Growth In vitro and In vivo.
      Authors: Ren W, Korchin B, Zhu QS, Wei C, Dicker A, Heymach J, Lazar A, Pollock RE, Lev D
      Clin. Cancer Res., 2008;14(9):2785-2795.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Modification of the primary tumor microenvironment by transforming growth factor alpha-epidermal growth factor receptor signaling promotes metastasis in an orthotopic colon cancer model.
      Authors: Sasaki T, Nakamura T, Rebhun RB, Cheng H, Hale KS, Tsan RZ, Fidler IJ, Langley RR
      Am. J. Pathol., 2008;173(1):205-16.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts: implications for osteolytic bone metastases.
      Authors: Zhu J, Jia X, Xiao G, Kang Y, Partridge NC, Qin L
      J. Biol. Chem., 2007;282(37):26656-64.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    15. Alteration of the serum levels of the epidermal growth factor receptor and its ligands in patients with non-small cell lung cancer and head and neck carcinoma.
      Authors: Lemos-Gonzalez Y, Rodriguez-Berrocal FJ, Cordero OJ, Gomez C, Paez de la Cadena M
      Br. J. Cancer, 2007;96(10):1569-78.
      Species: Human
      Sample Type: Serum
    16. The response of human epithelial cells to TNF involves an inducible autocrine cascade.
      Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
      Cell, 2006;124(6):1225-39.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
      Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
      J. Immunol., 2005;175(9):6177-89.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Mek1 alters epidermal growth and differentiation.
      Authors: Scholl FA, Dumesic PA
      Cancer Res., 2004;64(17):6035-40.
      Species: Porcine
      Sample Type: Cell Culture Supernates
    Expand to show all
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 397 for Human TGF-alpha

    Ctrl QC168
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    Supplemental ELISA Products
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    Quantikine Wash Buffer 1

    ELISA WA126 5
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