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Human TGF-beta 1 DuoSet ELISA

Name: Human TGF-beta 1 DuoSet ELISA
Brand: R&D Systems
SKU: DY240
Price: 890 USD
Availability: InStock
Rating: 4.142857142857143 (14 reviews)

R&D Systems | Catalog # DY240

(14)

Citations (414)

Product Documents

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Product Summary
Product Specifications
Scientific Data
Kit Contents
Other Reagents Required
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

31.2-2000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human TGF-beta 1 DuoSet ELISA Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

View all TGF-beta 1 ELISA Kits »

Product Summary for Human TGF-beta 1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant TGF-ß1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human TGF-beta 1 DuoSet ELISA

Human TGF-beta 1 ELISA Standard Curve

Kit Contents for Human TGF-beta 1 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 1

TGF-beta 1 (Transforming Growth Factor beta 1) is a pleiotrophic cytokine that regulates immune function, proliferation, and epithelial-mesenchymal transition. TGF-beta 1 associates with LAP to form a latent complex. It can be is activated from latency by plasmin, matrix metalloproteases, thrombospondin 1, and a subset of integrins. TGF-beta 1 signals through complexes of TGF-beta RII with TGF-beta RI/ALK-5 or ALK-1. TGF-beta signaling is modulated by the accessory receptors TGF-beta RIII/Betaglycan and Endoglin/CD105.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Entrez Gene IDs

7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)

Gene Symbol

TGFB1

Additional TGF-beta 1 Products

Product Documents for Human TGF-beta 1 DuoSet ELISA

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TGF-beta 1 DuoSet ELISA

For research use only

Related Research Areas

Cancer Biomarkers

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

Early Mesodermal Lineage Markers

Hypoxia Inducible Factors (HIFs)

Inflammatory Mediators

Microglia Activation Markers

Myeloid-derived Suppressor Cells (MDSC)

Preeclampsia

Regulatory T Cells (Tregs)

Citations for Human TGF-beta 1 DuoSet ELISA

Customer Reviews for Human TGF-beta 1 DuoSet ELISA (14)

4.1 out of 5

14 Customer Ratings

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Human TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Adult brain

Verified Customer | Posted 11/16/2025
Human TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Cell culture supernatant

Verified Customer | Posted 05/12/2021
Human TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Serum and Plasma

Verified Customer | Posted 03/29/2021

we used this kit to quantify human TGF beta 1 in human healthy controls sera, it produces a very good standard curve, levels of TGF beta in in healthy controls are very low.
Human TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: EDTA Plasma

Verified Customer | Posted 11/12/2020

Very good performance of the kit, we diluted our EDTA plasma samples 1:20 and applied the kit on a 384-well format. Very reliable and replicates are tight.
Name: Anonymous

Sample Tested: Porcine Serum and Serum

Verified Customer | Posted 10/05/2020
Human TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Serum-free Cell Culture Supernates

Verified Customer | Posted 10/29/2019

Should mention this kit detects active TGF beta1 only in the description. Need buy activation kit for total TGF beta1 (including the latent form)
Human TGF-beta 1 DuoSet ELISA

Name: Tim Stachowski

Sample Tested: Purified Standard

Verified Customer | Posted 08/23/2019
Name: Anonymous

Sample Tested: Cell Culture Media

Verified Customer | Posted 04/05/2019
Human TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Serum and Plasma

Verified Customer | Posted 10/11/2018
Human TGF-beta 1 DuoSet ELISA

Name: Maria Yanez

Sample Tested: THP-1 human acute monocytic leukemia cell line

Verified Customer | Posted 05/10/2018

I differentiated my THP-1 cells to macrophages and polarized using IL-4 and IL-13. Then I checked the TGF-beta and i have in trouble to detect the concentration for some of my samples. Then I always verify my experiments, and for the other assays I detected tgf-beta
Name: Maria Yanez

Sample Tested: THP-1 human acute monocytic leukemia cell line

Verified Customer | Posted 07/17/2017
Human TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Adult lung

Verified Customer | Posted 05/22/2017
Human TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Human EDTA plasma

Verified Customer | Posted 04/03/2017
Human TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Human dental periodontal ligament fibroblasts

Verified Customer | Posted 07/05/2016

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Protocols

View specific protocols for Human TGF-beta 1 DuoSet ELISA (DY240):

ACTIVATION REAGENT PREPARATION

To activate latent TGF-ß1 to the immunoreactive form, prepare the following solutions for acid activation and neutralization. The solutions may be stored in polypropylene bottles at room temperature for up to one month.

Caution: Wear protective clothing and safety glasses during preparation or use of these reagents. Refer to the appropriate MSDS before use.

1 N HCl (100 mL) - To 91.67 mL of deionized water, slowly add 8.33 mL of 12 N HCl. Mix well.

1.2 N NaOH/0.5 M HEPES (100 mL) - To 75 mL of deionized water, slowly add 12 mL of 10 N NaOH. Mix well. Add 11.9 g of HEPES. Mix well. Bring final volume to 100 mL with deionized water.

TGF-β1 SAMPLE ACTIVATION

To activate latent TGF-β1 to immunoreactive TGF-β1, follow the activation procedure outlined below. Assay samples after neutralization (pH 7.2-7.6). Use polypropylene test tubes.

Note: Do not activate the kit standards. The kit standards contain active recombinant TGF-β1.

Cell Culture Supernates	Serum/Plasma
To 100 μL of cell culture supernate, add 20 μL of 1 N HCI.	To 40 μL of serum/plasma, add 20 μL of 1 N HCI.
Mix well.	Mix well.
Incubate 10 minutes at room temperature.	Incubate 10 minutes at room temperature.
Neutralize the acidified sample by adding 20 μL of 1.2 N NaOH/0.5 M HEPES.	Neutralize the acidified sample by adding 20 μL of 1.2 N NaOH/0.5 M HEPES.
Mix well.	Mix well.
Assay immediately.	Prior to the assay, dilute the activated sample 20-fold with Reagent Diluent.*
The concentration read off the standard curve must be multiplied by the dilution factor, 1.4.	The concentration read off the standard curve must be multiplied by the appropriate dilution factor, 40.

*A suggested 20-fold dilution is 10 μL of activated sample + 190 μL of Reagent Diluent.

GENERAL ELISA PROTOCOL

Plate Preparation

Dilute the Capture Antibody (to the working concentration stated in the product datasheet ) in PBS without carrier protein. Immediately coat a 96-well microplate with 100 µL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 µL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
Block each well of the microplate as recommended in the product datasheet. Incubate at room temperature for a minimum of 1 hour.

Note: The recommended Reagent Diluent typically contains 1% BSA. Some DuoSet Development Kits require alternative blocking agents, or for plates to be blocked overnight with a higher percentage of BSA, please see the product datasheet for details.
Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

PRECAUTION
The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

Assay Procedure

Add 100 µL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
Repeat the aspiration/wash as in step 2 of Plate Preparation.
Add 100 µL of the Detection Antibody, diluted in Reagent Diluent (as recommended in the product datasheet), to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
Repeat the aspiration/wash as in step 2 of Plate Preparation.
Add 100 µL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
Repeat the aspiration/wash as in step 2.
Add 100 µL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
Add 50 µL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.