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Human TGF-beta 1 DuoSet ELISA

Catalog #: DY240
163 Citations 10 Reviews Datasheet / COA / SDS
Human TGF-beta 1 ELISA Standard Curve
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Citations (163)
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human TGF-beta 1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant TGF-ß1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 1 (5 plates): (Catalog # DY007) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 1.

The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4


Reagent Diluent: (Catalog # DY997), or 1.4% delipidized bovine serum, 0.05% Tween 20 in PBS, pH 7.2-7.4, 0.2 μm filtered.

Blocking Buffer: (Catalog # DY004), or 5% Tween 20 in PBS, pH 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Activation Reagents Required:

Sample Activation Kit 1: 3 vials (10 mL/vial) of 1N HCL and 3 vials (10 mL/vial) of 1.2 N NaOH/1M HEPES (R&D Systems, Catalog # DY010).

Scientific Data

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Human TGF-beta 1 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 1

Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.

Long Name:
Transforming Growth Factor beta 1
Entrez Gene IDs:
7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
Alternate Names:
CEDLAP; DPD1; latency-associated peptide; TGF beta; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

Assay Procedure

ACTIVATION REAGENT PREPARATION

To activate latent TGF-ß1 to the immunoreactive form, prepare the following solutions for acid activation and neutralization. The solutions may be stored in polypropylene bottles at room temperature for up to one month.

Caution: Wear protective clothing and safety glasses during preparation or use of these reagents. Refer to the appropriate MSDS before use.

1 N HCl (100 mL) - To 91.67 mL of deionized water, slowly add 8.33 mL of 12 N HCl. Mix well.

1.2 N NaOH/0.5 M HEPES (100 mL) - To 75 mL of deionized water, slowly add 12 mL of 10 N NaOH. Mix well. Add 11.9 g of HEPES. Mix well. Bring final volume to 100 mL with deionized water.

TGF-β1 SAMPLE ACTIVATION

To activate latent TGF-β1 to immunoreactive TGF-β1, follow the activation procedure outlined below. Assay samples after neutralization (pH 7.2-7.6). Use polypropylene test tubes.

Note: Do not activate the kit standards. The kit standards contain active recombinant TGF-β1.

Cell Culture Supernates Serum/Plasma
To 100 μL of cell culture supernate, add 20 μL of 1 N HCI. To 40 μL of serum/plasma, add 20 μL of 1 N HCI.
Mix well. Mix well.
Incubate 10 minutes at room temperature. Incubate 10 minutes at room temperature.
Neutralize the acidified sample by adding 20 μL of 1.2 N NaOH/0.5 M HEPES. Neutralize the acidified sample by adding 20 μL of 1.2 N NaOH/0.5 M HEPES.
Mix well. Mix well.
Assay immediately. Prior to the assay, dilute the activated sample 20-fold with Reagent Diluent.*
The concentration read off the standard curve must be multiplied by the dilution factor, 1.4. The concentration read off the standard curve must be multiplied by the appropriate dilution factor, 90.

 

*A suggested 20-fold dilution is 10 μL of activated sample + 190 μL of Reagent Diluent.

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody (to the working concentration stated in the product datasheet ) in PBS without carrier protein. Immediately coat a 96-well microplate with 100 µL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 µL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block each well of the microplate as recommended in the product datasheet. Incubate at room temperature for a minimum of 1 hour.

    Note: The recommended Reagent Diluent typically contains 1% BSA. Some DuoSet Development Kits require alternative blocking agents, or for plates to be blocked overnight with a higher percentage of BSA, please see the product datasheet for details.
     
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

 

PRECAUTION
The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

Assay Procedure

  1. Add 100 µL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 µL of the Detection Antibody, diluted in Reagent Diluent (as recommended in the product datasheet), to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 µL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 µL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 µL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human TGF-beta 1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

163 Citations: Showing 1 - 10
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  1. SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-beta signaling
    Authors: SB Biering, FT Gomes de S, LV Tjang, F Pahmeier, C Zhu, R Ruan, SF Blanc, TS Patel, CM Worthingto, DR Glasner, B Castillo-R, V Servellita, NTN Lo, MP Wong, CM Warnes, DR Sandoval, TM Clausen, YA Santos, DM Fox, V Ortega, AM Näär, RS Baric, SA Stanley, HC Aguilar, JD Esko, CY Chiu, JE Pak, PR Beatty, E Harris
    Nature Communications, 2022;13(1):7630.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Role of Betaglycan in TGF-beta Signaling and Wound Healing in Human Endometriotic Epithelial Cells and in Endometriosis
    Authors: AN Mwaura, MA Riaz, JB Maoga, E Mecha, COA Omwandho, G Scheiner-B, I Meinhold-H, L Konrad
    Biology, 2022;11(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Therapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Model
    Authors: AL Graça, RMA Domingues, I Calejo, M Gómez-Flor, ME Gomes
    International Journal of Molecular Sciences, 2022;23(6):.
    Species: Human
    Sample Types: Extracellular Vesicles
  4. Optimization of loading protocols for tissue engineering experiments
    Authors: YD Ladner, AR Armiento, EJ Kubosch, JG Snedeker, MJ Stoddart
    Scientific Reports, 2022;12(1):5094.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Anti-inflammatory effects of an autologous gold-based serum therapy in osteoarthritis patients
    Authors: J Feldt, AJ Donaubauer, J Welss, U Schneider, US Gaipl, F Paulsen
    Scientific Reports, 2022;12(1):3560.
    Species: Human
    Sample Types: Serum
  6. Increased serum LOXL2 concentration in pelvic inflammatory disease with pelvic adhesion
    Authors: C Xie, B Tang, K Wu, Q Meng, F Wang
    Oncogene, 2022;22(1):59.
    Species: Human
    Sample Types: Serum
  7. Tethered TGF-beta1 in a Hyaluronic Acid-Based Bioink for Bioprinting Cartilaginous Tissues
    Authors: J Hauptstein, L Forster, A Nadernezha, J Groll, J Te beta mar, T Blunk
    International Journal of Molecular Sciences, 2022;23(2):.
    Species: Human
    Sample Types: Recombinant Protein
  8. Cytokine Profile and Anti-Inflammatory Activity of a Standardized Conditioned Medium Obtained by Coculture of Monocytes and Mesenchymal Stromal Cells (PRS CK STORM)
    Authors: JP Lapuente, A Blázquez-M, J Marco-Brua, G Gómez, P Desportes, J Sanz, P Fernández, M García-Gil, F Bermejo, JV San Martín, A Algaba, JC De Gregori, D Lapuente, A De Gregori, B Lapuente, MV Andrés, A Anel
    Biomolecules, 2022;12(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Parathyroid Hormone Induces Human Valvular Endothelial Cells Dysfunction That Impacts the Osteogenic Phenotype of Valvular Interstitial Cells
    Authors: M Vadana, S Cecoltan, L Ciortan, RD Macarie, AC Mihaila, MM Tucureanu, AM Gan, M Simionescu, I Manduteanu, I Droc, E Butoi
    International Journal of Molecular Sciences, 2022;23(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Nucleoporin-93 reveals a common feature of aggressive breast cancers: robust nucleocytoplasmic transport of transcription factors
    Authors: NB Nataraj, A Noronha, JS Lee, S Ghosh, HR Mohan Raju, A Sekar, B Zuckerman, M Lindzen, E Tarcitano, S Srivastava, M Selitrenni, I Livneh, D Drago-Garc, O Rueda, C Caldas, S Lev, T Geiger, A Ciechanove, I Ulitsky, R Seger, E Ruppin, Y Yarden
    Cell Reports, 2022;38(8):110418.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Prediction of emergency cerclage outcomes in women with cervical insufficiency: The role of inflammatory, angiogenic, and extracellular matrix-related proteins in amniotic fluid
    Authors: KN Lee, KH Park, YM Kim, I Cho, TE Kim
    PLoS ONE, 2022;17(5):e0268291.
    Species: Human
    Sample Types: Amniotic Fluid
  12. Extracellular matrix of early pulmonary fibrosis modifies the polarization of alveolar macrophage
    Authors: Y Zhang, L Zhu, J Hong, C Chen
    International immunopharmacology, 2022;111(0):109179.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Plasma proteomic analysis to identify potential biomarkers of histologic chorioamnionitis in women with preterm premature rupture of membranes
    Authors: JE Lee, K Dan, HJ Kim, YM Kim, KH Park
    PLoS ONE, 2022;17(7):e0270884.
    Species: Human
    Sample Types: Plasma
  14. Preparation and growth factor characterization of cord blood-derived plasma, serum, growth factor-rich plasma and induced serum
    Authors: ME Rhéaume, J Perreault, D Fournier, P Trépanier
    Cytokine, 2022;149(0):155756.
    Species: Human
    Sample Types: Serum
  15. Growth Factor Release within Liquid and Solid PRF
    Authors: K Zwittnig, B Kirnbauer, N Jakse, P Schlenke, I Mischak, S Ghanaati, S Al-Maawi, D Végh, M Payer, TA Zrnc
    Journal of Clinical Medicine, 2022;11(17):.
    Species: Human
    Sample Types: Serum
  16. Minimal Development of Liver Fibrosis in Adult Tolerant Liver Transplant Recipients Late After Immunosuppressive Drug Weaning and Transplantation
    Authors: AA Duizendstr, RJ De Knegt, NMA Nagtzaam, MGH Betjes, WA Dik, NHR Litjens, J Kwekkeboom
    Transplantation Proceedings, 2022;0(0):.
    Species: Human
    Sample Types: Serum
  17. Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK
    Authors: C Ge, J Tan, X Dai, Q Kuang, S Zhong, L Lai, C Yi, Y Sun, J Luo, C Zhang, L Zhu, B Wang, M Xu
    Oncogene, 2022;13(1):5945.
    Species: Human
    Sample Types: Serum
  18. Eribulin normalizes pancreatic cancer-associated fibroblasts by simulating selected features of TGFbeta inhibition
    Authors: T Luong, E Cukierman
    BMC Cancer, 2022;22(1):1255.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis
    Authors: S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Journal of ovarian research, 2022;15(1):87.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. SerpinB3 drives cancer stem cell survival in glioblastoma
    Authors: A Lauko, J Volovetz, SM Turaga, D Bayik, DJ Silver, K Mitchell, EE Mulkearns-, DC Watson, K Desai, M Midha, J Hao, K McCortney, A Steffens, U Naik, MS Ahluwalia, S Bao, C Horbinski, JS Yu, JD Lathia
    Cell Reports, 2022;40(11):111348.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Development of an Electrochemical CCL5 Chemokine Immunoplatform for Rapid Diagnosis of Multiple Sclerosis
    Authors: S Guerrero, E Sánchez-Ti, L Agüí, A González-C, P Yáñez-Sede, JM Pingarrón
    Biosensors, 2022;12(8):.
    Species: Human
    Sample Types: Serum
  22. Utilization of ex vivo tissue model to study skin regeneration following microneedle stimuli
    Authors: X Liu, R Barresi, M Kaminer, K Qian, F Thillou, M Bataillon, IC Liao, Q Zheng, C Bouez
    Scientific Reports, 2022;12(1):18115.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. A TGFbetaR inhibitor represses keratin-7 expression in 3D cultures of human salivary gland progenitor cells
    Authors: EW Fowler, EJ van Venroo, RL Witt, X Jia
    Scientific Reports, 2022;12(1):15008.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Melanoma Stem Cells Educate Neutrophils to Support Cancer Progression
    Authors: M Anselmi, F Fontana, M Marzagalli, N Gagliano, M Sommariva, P Limonta
    Cancers, 2022;14(14):.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Immortalised canine buccal epithelial cells' CXCL8 secretion is affected by allergen extracts, Toll-like receptor ligands, IL-17A and calcitriol
    Authors: M Pelst, C Höbart, H de Rooster, B Devriendt, E Cox
    Veterinary research, 2022;53(1):72.
    Species: Canine
    Sample Types: Cell Culture Supernates
  26. Neutrophils promote T-cell activation through the regulated release of CD44-bound Galectin-9 from the cell surface during HIV infection
    Authors: G Dunsmore, EP Rosero, S Shahbaz, DM Santer, J Jovel, P Lacy, S Houston, S Elahi
    PloS Biology, 2021;19(8):e3001387.
    Species: Human
    Sample Types: Plasma
  27. Regulation of Fibroblast Activation Protein by Transforming Growth Factor Beta-1 in Glioblastoma Microenvironment
    Authors: E Krepela, Z Vanickova, P Hrabal, M Zubal, B Chmielova, E Balaziova, P Vymola, I Matrasova, P Busek, A Sedo
    International Journal of Molecular Sciences, 2021;22(3):.
    Species: Human
    Sample Types: Tissue Homogenates
  28. TGF&beta1 induces in-vitro and ex-vivo angiogenesis through VEGF production in human ovarian follicular fluid-derived granulosa cells during in-vitro fertilization cycle
    Authors: TH Lai, HT Chen, WB Wu
    Journal of reproductive immunology, 2021;145(0):103311.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Severe COVID-19 Patients Show an Increase in Soluble TNFR1 and ADAM17, with a Relationship to Mortality
    Authors: Y Palacios, A Ruiz, LA Ramón-Luin, R Ocaña-Guzm, O Barreto-Ro, A Sánchez-Mo, B Tecuatzi-C, AG Regalado-G, RD Pineda-Gud, A García-Mar, F Juárez-Her, JP Farias-Con, I Fricke-Gal, G Pérez-Rubi, R Falfán-Val, I Buendia-Ro, K Medina-Que, L Chavez-Gal
    International Journal of Molecular Sciences, 2021;22(16):.
    Species: Human
    Sample Types: Serum
  30. The effects of resveratrol on the expression of VEGF, TGF-&beta, and MMP-9 in endometrial stromal cells of women with endometriosis
    Authors: T Arablou, N Aryaeian, S Khodaverdi, R Kolahdouz-, Z Moradi, N Rashidi, AA Delbandi
    Scientific Reports, 2021;11(1):6054.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Palladin isoforms 3 and 4 regulate cancer-associated fibroblast pro-tumor functions in pancreatic ductal adenocarcinoma
    Authors: JI Alexander, DB Vendramini, R Francescon, T Luong, J Franco-Bar, N Shah, JC Gardiner, E Nicolas, KS Raghavan, E Cukierman
    Scientific Reports, 2021;11(1):3802.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Regulation of Cellular Senescence Is Independent from Profibrotic Fibroblast-Deposited ECM
    Authors: KEC Blokland, H Habibie, T Borghuis, GJ Teitsma, M Schuliga, BN Melgert, DA Knight, CA Brandsma, SD Pouwels, JK Burgess
    Cells, 2021;10(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Th17/Treg-Related Intracellular Signaling in Patients with Chronic Obstructive Pulmonary Disease: Comparison between Local and Systemic Responses
    Authors: JD Lourenço, WR Teodoro, DF Barbeiro, APP Velosa, LEF Silva, JB Kohler, AR Moreira, MV Aun, IC da Silva, FLA Fernandes, EM Negri, JL Gross, IFLC Tibério, JT Ito, FDTQS Lopes
    Cells, 2021;10(7):.
    Species: Human
    Sample Types: Tissue Homogenates
  34. Human Adipose Tissue-Derived Mesenchymal Stromal Cells Inhibit CD4+ T Cell Proliferation and Induce Regulatory T Cells as Well as CD127 Expression on CD4+CD25+ T Cells
    Authors: A Fiori, S Uhlig, H Klüter, K Bieback
    Cells, 2021;10(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Precision targeting of the plasminogen activator inhibitor-1 mechanism increases efficacy of fibrinolytic therapy in empyema
    Authors: G Florova, RA Girard, AO Azghani, K Sarva, A Buchanan, S Karandasho, CJ DeVera, D Morris, M Chamiso, K Koenig, DB Cines, S Idell, AA Komissarov
    Physiological Reports, 2021;9(9):e14861.
    Species: Rabbit
    Sample Types: Pleural Fluid
  36. Changes in liver steatosis in HIV-positive women are associated with the BMI, but not with biomarkers
    Authors: R Fernandez-, MW Plankey, D Ware, J Bordon
    Cytokine, 2021;0(0):155573.
    Species: Human
    Sample Types: Plasma
  37. Serum protein triplet TGF-&beta1, TIMP-1, and YKL-40 serve as diagnostic and prognostic profile for astrocytoma
    Authors: R Urbanavi?i, R Zabitait?, A Kriš?iukai, VP Deltuva, D Skiriut?
    Scientific Reports, 2021;11(1):13100.
    Species: Human
    Sample Types: Serum
  38. Latent TGFbeta-binding proteins regulate UCP1 expression and function via TGFbeta2
    Authors: D Halbgebaue, J Roos, JB Funcke, H Neubauer, BS Hamilton, E Simon, EZ Amri, KM Debatin, M Wabitsch, P Fischer-Po, D Tews
    Molecular Metabolism, 2021;53(0):101336.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. An In Vitro System to Study the Effect of Subchondral Bone Health on Articular Cartilage Repair in Humans
    Authors: T Hopkins, KT Wright, NJ Kuiper, S Roberts, P Jermin, P Gallacher, JH Kuiper
    Cells, 2021;10(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Proteomic identification of novel plasma biomarkers associated with spontaneous preterm birth in women with preterm labor without infection/inflammation
    Authors: JE Lee, KH Park, HJ Kim, YM Kim, JW Choi, S Shin, KN Lee
    PLoS ONE, 2021;16(10):e0259265.
    Species: Human
    Sample Types: Plasma
  41. Longitudinal relationships between polycyclic aromatic hydrocarbons exposure and heart rate variability: Exploring the role of transforming�growth factor-beta in a general Chinese population
    Authors: J Ma, Q Tan, X Nie, M Zhou, B Wang, X Wang, M Cheng, Z Ye, Y Xie, D Wang, W Chen
    Journal of Hazardous Materials, 2021;0(0):127770.
    Species: Human
    Sample Types: Plasma
  42. The noncoding MIR100HG RNA enhances the autocrine function of transforming growth factor &beta signaling
    Authors: P Papoutsogl, DM Rodrigues-, A Morén, A Bergman, F Pontén, C Coulouarn, L Caja, CH Heldin, A Moustakas
    Oncogene, 2021;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. Intramammary treatment using allogeneic pure platelet-rich plasma in cows with subclinical mastitis caused by Gram-positive bacteria
    Authors: PC Duque-Madr, J Velasco-Bo, A Ceballos-M, C López, JU Carmona
    Scientific Reports, 2021;11(1):23737.
    Species: Bovine
    Sample Types: Plasma
  44. Impact and Possible Mechanism(s) of Adipose Tissue-Derived Mesenchymal Stem Cells on T-Cell Proliferation in Patients With Rheumatic Disease
    Authors: E Kuca-Warna, M Olesi?ska, P Szcz?sny, E Kontny
    Frontiers in Physiology, 2021;12(0):749481.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Skin Substitute Preparation Method Induces Immunomodulatory Changes in Co-Incubated Cells through Collagen Modification
    Authors: J Holl, C Pawlukiani, J Corton Rui, D Groth, K Grubczak, HR Hady, J Dadan, J Reszec, S Czaban, C Kowalewski, M Moniuszko, A Eljaszewic
    Pharmaceutics, 2021;13(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-beta signaling
    Authors: SB Biering, FTG de Sousa, LV Tjang, F Pahmeier, R Ruan, SF Blanc, TS Patel, CM Worthingto, DR Glasner, B Castillo-R, V Servellita, NTN Lo, MP Wong, CM Warnes, DR Sandoval, TM Clausen, YA Santos, V Ortega, HC Aguilar, JD Esko, CY Chui, JE Pak, PR Beatty, E Harris
    bioRxiv : the preprint server for biology, 2021;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Inhibition of miR-155 Promotes TGF-beta Mediated Suppression of HIV Release in the Cervical Epithelial Cells
    Authors: J Gokavi, S Sadawarte, A Shelke, U Kulkarni-K, M Thakar, V Saxena
    Viruses, 2021;13(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. Odontogenic infection by Porphyromonas gingivalis exacerbates fibrosis in NASH via hepatic stellate cell activation
    Authors: A Nagasaki, S Sakamoto, C Chea, E Ishida, H Furusho, M Fujii, T Takata, M Miyauchi
    Sci Rep, 2020;10(1):4134.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Impaired Release of Neutrophil Extracellular Traps and Anemia-Associated T Cell Deficiency in Hereditary Hemorrhagic Telangiectasia
    Authors: F Droege, E Pylaeva, E Siakaeva, S Bordbari, I Spyra, K Thangavelu, C Lueb, M Domnich, S Lang, U Geisthoff, J Jablonska
    J Clin Med, 2020;9(3):.
    Species: Human
    Sample Types: Plasma
  50. GLP-1 receptor agonist ameliorates experimental lung fibrosis
    Authors: J Fandiño, L Toba, LC González-M, Y Diz-Chaves, F Mallo
    Sci Rep, 2020;10(1):18091.
    Species: Rat
    Sample Types: Plasma
  51. A-Kinase Anchoring Proteins Diminish TGF-&beta1/Cigarette Smoke-Induced Epithelial-To-Mesenchymal Transition
    Authors: H Zuo, M Trombetta-, IH Heijink, CHTJ van der Ve, L Hesse, KN Faber, WJ Poppinga, H Maarsingh, VO Nikolaev, AM Schmidt
    Cells, 2020;9(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis
    Authors: JH Ko, HJ Kim, HJ Jeong, HJ Lee, JY Oh
    Cell Rep, 2020;30(11):3806-3820.e6.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Growth Factors Delivery System for Skin Regeneration: An Advanced Wound Dressing
    Authors: M Nardini, S Perteghell, L Mastracci, F Grillo, G Marrubini, E Bari, M Formica, C Gentili, R Cancedda, ML Torre, M Mastrogiac
    Pharmaceutics, 2020;12(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Are the Immune Properties of Mesenchymal Stem Cells from Wharton's Jelly Maintained during Chondrogenic Differentiation?
    Authors: C Voisin, G Cauchois, L Reppel, C Laroye, L Louarn, C Schenowitz, P Sonon, I Poras, V Wang, E D Carosell, N Benkirane-, P Moreau, N Rouas-Frei, D Bensoussan, C Huselstein
    J Clin Med, 2020;9(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Exploring the Role of C-C Motif Chemokine Ligand-2 Single Nucleotide Polymorphism in Pulmonary Tuberculosis: A Genetic Association Study from North India
    Authors: SK Biswas, M Mittal, E Sinha, V Singh, N Arela, B Bajaj, PK Tiwari, VM Katoch, KK Mohanty
    Journal of Immunology Research, 2020;2020(0):1019639.
    Species: Human
    Sample Types: Serum
  56. Renal cell tumors convert natural killer cells to a proangiogenic phenotype
    Authors: Y Guan, CB Chambers, T Tabatabai, H Hatley, KR Delfino, K Robinson, SR Alanee, S Ran, DS Torry, A Wilber
    Oncotarget, 2020;11(26):2571-2585.
    Species: Human
    Sample Types: Serum
  57. Circulating biomarkers and outcomes from a randomised phase 2 trial of gemcitabine versus capecitabine-based chemoradiotherapy for pancreatic cancer
    Authors: F Willenbroc, CM Cox, EE Parkes, CS Wilhelm-Be, AG Abraham, R Owens, A Sabbagh, CM Jones, DLI Hughes, T Maughan, CN Hurt, EE O'Neill, S Mukherjee
    Br J Cancer, 2020;0(0):.
    Species: Human
    Sample Types: Plasma
  58. Regional hyperthermia enhances mesenchymal stem cell recruitment to tumor stroma: Implications for mesenchymal stem cell-based tumor therapy
    Authors: M Tutter, C Schug, KA Schmohl, S Urnauer, C Kitzberger, N Schwenk, M Petrini, C Zach, S Ziegler, P Bartenstei, WA Weber, G Multhoff, E Wagner, LH Lindner, PJ Nelson, C Spitzweg
    Mol Ther, 2020;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. TGF-&beta in the Secretome of Irradiated Peripheral Blood Mononuclear Cells Supports In Vitro Osteoclastogenesis
    Authors: L Panahipour, Z Kargarpour, M Laggner, M Mildner, HJ Ankersmit, R Gruber
    Int J Mol Sci, 2020;21(22):.
    Species: Human
    Sample Types: Cell Culture Supernates
  60. The role of EMMPRIN/CD147 in regulating angiogenesis in patients with psoriatic arthritis
    Authors: MA Rahat, M Safieh, E Simanovich, E Pasand, T Gazitt, A Haddad, M Elias, D Zisman
    Arthritis Res Ther, 2020;22(1):240.
    Species: Human
    Sample Types: Serum
  61. The NRF2 stimulating agent, tin protoporphyrin, activates protective cytokine pathways in healthy human subjects and in patients with chronic kidney disease
    Authors: RA Zager, ACM Johnson
    Physiol Rep, 2020;8(18):e14566.
    Species: Human
    Sample Types: Plasma
  62. Renal cell tumors convert natural killer cells to a proangiogenic phenotype
    Authors: Y Guan, CB Chambers, T Tabatabai, H Hatley, KR Delfino, K Robinson, SR Alanee, S Ran, DS Torry, A Wilber
    Oncotarget, 2020;11(26):2571-2585.
    Species: Human
    Sample Types: Serum
  63. Downregulation of epithelial DUOX1 in Chronic Obstructive Pulmonary Disease contributes to disease pathogenesis
    Authors: C Schiffers, C van de Wet, RA Bauer, A Habibovic, M Hristova, CM Dustin, S Lambrichts, PM Vacek, EF Wouters, NL Reynaert, A van der Vl
    JCI Insight, 2020;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. Patient-derived and artificial ascites have minor effects on MeT-5A mesothelial cells and do not facilitate ovarian cancer cell adhesion
    Authors: M Estermann, YL Huang, D Septiadi, D Ritz, CY Liang, F Jacob, B Drasler, A Petri-Fink, V Heinzelman, B Rothen-Rut
    PLoS ONE, 2020;15(12):e0241500.
    Species: Human
    Sample Types: Abdominal Fluid
  65. Anti-fibrotic effect of decorin in peritoneal dialysis and PD-associated peritonitis
    Authors: N Jiang, Q Zhang, MK Chau, MS Yip, SL Lui, S Liu, KM Chu, HY Ngan, TM Chan, S Yung
    EBioMedicine, 2020;52(0):102661.
    Species: Human
    Sample Types: Dialysate
  66. Human interleukin-4-treated regulatory macrophages promote epithelial wound healing and reduce colitis in a mouse model
    Authors: TS Jayme, G Leung, A Wang, ML Workentine, S Rajeev, A Shute, BE Callejas, N Mancini, PL Beck, R Panaccione, DM McKay
    Sci Adv, 2020;6(23):eaba4376.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. Liposomes loaded with transforming growth factor &beta1 promote odontogenic differentiation of dental pulp stem cells
    Authors: L Jiang, WN Ayre, GE Melling, B Song, X Wei, AJ Sloan, X Chen
    J Dent, 2020;103(0):103501.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. Immune modulation by complement receptor 3-dependent human monocyte TGF-beta1-transporting vesicles
    Authors: LD Halder, EAH Jo, MZ Hasan, M Ferreira-G, T Krüger, M Westermann, DI Palme, G Rambach, N Beyersdorf, C Speth, ID Jacobsen, O Kniemeyer, B Jungnickel, PF Zipfel, C Skerka
    Nat Commun, 2020;11(1):2331.
    Species: Human
    Sample Types: Extracellular Vesicles
  69. Blood cytokine, chemokine and growth factor profiling in a cohort of pregnant women from tropical countries
    Authors: C Dobaño, A Bardají, S Kochar, SK Kochar, N Padilla, M López, HW Unger, M Ome-Kaius, ME Castellano, M Arévalo-He, D Hans, FE Martínez-E, C Bôtto-Mene, A Malheiros, M Desai, A Casellas, CE Chitnis, S Rogerson, I Mueller, C Menéndez, P Requena
    Cytokine, 2019;125(0):154818.
    Species: Human
    Sample Types: Plasma
  70. Inflammatory Bowel Disease Types Differ in Markers of Inflammation, Gut Barrier and in Specific Anti-Bacterial Response
    Authors: S Coufal, N Galanova, L Bajer, Z Gajdarova, D Schierova, Z Jiraskova, K Kostovciko, Z Jackova, Z Stehlikova, P Drastich, H Tlaskalova, M Kverka
    Cells, 2019;8(7):.
    Species: Human
    Sample Types: Serum
  71. Type 1 diabetic mellitus patients with increased atherosclerosis risk display decreased CDKN2A/2B/2BAS gene expression in leukocytes
    Authors: S Martínez-H, V Sánchez-Ga, A Herrero-Ce, Á Vinué, JT Real, JF Ascaso, DJ Burks, H González-N
    J Transl Med, 2019;17(1):222.
    Species: Human
    Sample Types: Plasma
  72. Large-scale secretome analyses unveil the superior immunosuppressive phenotype of umbilical cord stromal cells as compared to other adult mesenchymal stromal cells
    Authors: A Islam, I Urbarova, JA Bruun, I Martinez-Z
    Eur Cell Mater, 2019;37(0):153-174.
    Species: Human
    Sample Types: Cell Culture Supernates
  73. Tensin1 expression and function in chronic obstructive pulmonary disease
    Authors: P Stylianou, K Clark, B Gooptu, D Smallwood, CE Brightling, Y Amrani, KM Roach, P Bradding
    Sci Rep, 2019;9(1):18942.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. Changes on the Structural Architecture and Growth Factor Release, and Degradation in Equine Platelet-Rich Fibrin Clots Cultured Over Time
    Authors: RF Jiménez-Ar, JU Carmona, M Prades
    J. Equine Vet. Sci., 2019;82(0):102789.
    Species: Equine
    Sample Types: Serum
  75. The Phenotype and Secretory Activity of Adipose-Derived Mesenchymal Stem Cells (ASCs) of Patients with Rheumatic Diseases
    Authors: E Kuca-Warna, U Skalska, I Janicka, U Musia?owic, K Bonek, P G?uszko, P Szcz?sny, M Olesi?ska, E Kontny
    Cells, 2019;8(12):.
    Species: Human
    Sample Types: Cell Culture Superantes
  76. A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer
    Authors: M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Cancers (Basel), 2019;11(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  77. Effect of Propofol on the Production of Inflammatory Cytokines by Human Polarized Macrophages
    Authors: T Kochiyama, X Li, H Nakayama, M Kage, Y Yamane, K Takamori, K Iwabuchi, E Inada
    Mediators Inflamm., 2019;2019(0):1919538.
    Species: Human
    Sample Types: Cell Culture Supernates
  78. Plasma Rich in Growth Factors (PRGF) Disrupt the Blood-Brain Barrier Integrity and Elevate Amyloid Pathology in the Brains of 5XFAD Mice
    Authors: QV Duong, ML Kintzing, WE Kintzing, IM Abdallah, AD Brannen, A Kaddoumi
    Int J Mol Sci, 2019;20(6):.
    Species: Human
    Sample Types: Plasma
  79. Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response
    Authors: H Rammal, C Bour, M Dubus, L Entz, L Aubert, SC Gangloff, S Audonnet, NB Bercu, F Boulmedais, C Mauprivez, H Kerdjoudj
    Int J Mol Sci, 2018;19(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  80. Tumor associated macrophages induce epithelial to mesenchymal transition via the EGFR/ERK1/2 pathway in head and neck squamous cell carcinoma
    Authors: L Gao, W Zhang, WQ Zhong, ZJ Liu, HM Li, ZL Yu, YF Zhao
    Oncol. Rep., 2018;40(5):2558-2572.
  81. Comparative Analysis of Different Platelet Lysates and Platelet Rich Preparations to Stimulate Tendon Cell Biology: An In Vitro Study
    Authors: F Klatte-Sch, T Schmidt, M Uckert, S Scheffler, U Kalus, M Rojewski, H Schrezenme, A Pruss, B Wildemann
    Int J Mol Sci, 2018;19(1):.
    Species: Human
    Sample Types: Plasma
  82. Plasmodium falciparum Treated with Artemisinin-based Combined Therapy Exhibits Enhanced Mutation, Heightened Cortisol and TNF-? Induction
    Authors: AO Idowu, S Bhattachar, S Gradus, W Oyibo, Z George, C Black, J Igietseme, AA Azenabor
    Int J Med Sci, 2018;15(13):1449-1457.
    Species: Human
    Sample Types: Plasma
  83. Exosomes Induce Fibroblast Differentiation into Cancer-associated Fibroblasts through TGF? Signaling
    Authors: C R Goulet, G Bernard, S Tremblay, S Chabaud, S Bolduc, F Pouliot
    Mol. Cancer Res., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  84. LINK-A lncRNA promotes migration and invasion of ovarian carcinoma cells by activating TGF-? pathway
    Authors: J Ma, M Xue
    Biosci. Rep., 2018;0(0):.
    Species: Human
    Sample Types: Plasma
  85. Helicobacter pylori-derived heat shock protein 60 increases the induction of regulatory T-cells associated with persistent infection
    Authors: WT Hsu, SY Ho, TY Jian, HN Huang, YL Lin, CH Chen, TH Lin, MS Wu, CJ Wu, YL Chan, KW Liao
    Microb. Pathog., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  86. PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia
    Authors: T Lopatina, E Favaro, C Grange, M Cedrino, A Ranghino, S Occhipinti, S Fallo, F Buffolo, DA Gaykalova, MM Zanone, R Romagnoli, G Camussi
    Sci Rep, 2018;8(1):17458.
    Species: Human
    Sample Types: Cell Culture Supernates
  87. Inhalation of the prodrug PI3K inhibitor CL27c improves lung function in asthma and fibrosis
    Authors: CC Campa, RL Silva, JP Margaria, T Pirali, MS Mattos, LR Kraemer, DC Reis, G Grosa, F Copperi, EM Dalmarco, RCP Lima-Júnio, S Aprile, V Sala, F Dal Bello, DS Prado, JC Alves-Filh, C Medana, GD Cassali, GC Tron, MM Teixeira, E Ciraolo, RC Russo, E Hirsch
    Nat Commun, 2018;9(1):5232.
  88. ??CD4effector/effector memory T cells differentiate into productively and latently infected central memory T cells by TGF?1 during HIV-1 infection
    Authors: KW Cheung, T Wu, SF Ho, YC Wong, L Liu, H Wang, Z Chen
    J. Virol., 2018;0(0):.
    Species: Bovine
    Sample Types: Media
  89. A model combining age, equivalent uniform dose and IL-8 may predict radiation esophagitis in patients with non-small cell lung cancer
    Authors: S Wang, J Campbell, MH Stenmark, P Stanton, J Zhao, MM Matuszak, RK Ten Haken, FM Kong
    Radiother Oncol, 2018;0(0):.
    Species: Human
    Sample Types: Plasma
  90. Inhibition of profibrotic microRNA-21 affects platelets and their releasate
    Authors: T Barwari, S Eminaga, U Mayr, R Lu, PC Armstrong, MV Chan, M Sahraei, M Fernández-, T Moreau, J Barallobre, M Lynch, X Yin, C Schulte, F Baig, R Pechlaner, SR Langley, A Zampetaki, P Santer, M Weger, R Plasenzott, M Schosserer, J Grillari, S Kiechl, J Willeit, AM Shah, C Ghevaert, TD Warner, C Fernández-, Y Suárez, M Mayr
    JCI Insight, 2018;3(21):.
    Species: Human
    Sample Types: Plasma
  91. Butyrate produced by gut commensal bacteria activates TGF-beta1 expression through the transcription factor SP1 in human intestinal epithelial cells
    Authors: C Martin-Gal, F Béguet-Cre, L Marinelli, A Jamet, F Ledue, HM Blottière, N Lapaque
    Sci Rep, 2018;8(1):9742.
    Species: Human
    Sample Types: Cell Culture Supernates
  92. Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton Jelly mesenchymal stromal cells
    Authors: MS Chen, TJ Wang, HC Lin, B Thierry
    New biotechnology, 2018;0(0):.
    Species: Human
    Sample Types: Platelet Lysates
  93. Crude leaf extracts of Piperaceae species downmodulate inflammatory responses by human monocytes
    Authors: AC Finato, TF Fraga-Silv, AUC Prati, AA de Souza J, BF Mazzeu, LG Felippe, RA Pinto, MA Golim, MSP Arruda, M Furlan, J Venturini
    PLoS ONE, 2018;13(6):e0198682.
    Species: Human
    Sample Types: Cell Culture Supernates
  94. Long-term cytokine and growth factor release from equine platelet-rich fibrin clots obtained with two different centrifugation protocols
    Authors: RF Jiménez-Ar, C López, ME Álvarez, C Giraldo, M Prades, JU Carmona
    Cytokine, 2017;97(0):149-155.
    Species: Equine
    Sample Types: Plasma
  95. Activated Tissue-Resident Mesenchymal Stromal Cells Regulate Natural Killer Cell Immune and Tissue-Regenerative Function
    Authors: RM Petri, A Hackel, K Hahnel, CA Dumitru, K Bruderek, SB Flohe, A Paschen, S Lang, S Brandau
    Stem Cell Reports, 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  96. In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens
    Authors: N Goli?, K Veljovi?, N Popovi?, J Djoki?, I Strahini?, I Mrvaljevi?, A Terzi?-Vid
    BMC Microbiol., 2017;17(1):108.
    Species: Human
    Sample Types: Cell Culture Supernates
  97. Neutrophil Extracellular Traps Reprogram IL-4/GM-CSF-Induced Monocyte Differentiation to Anti-inflammatory Macrophages
    Authors: AB Guimarães-, NC Rochael, F Oliveira, J Echevarria, EM Saraiva
    Front Immunol, 2017;8(0):523.
    Species: Human
    Sample Types: Cell Culture Supernates
  98. A nonsense mutation in TLR5 is associated with survival and reduced IL-10 and TNF-? levels in human Melioidosis
    Authors: P Chaichana, N Chantratit, F Brod, S Koosakulni, K Jenjaroen, S Chumseng, M Sumonwiriy, MN Burtnick, PJ Brett, P Teparrukku, D Limmathuro, NPJ Day, SJ Dunachie, TE West
    PLoS Negl Trop Dis, 2017;11(5):e0005587.
    Species: Human
    Sample Types: Plasma
  99. On the cytokine/chemokine network during Plasmodium vivax malaria: new insights to understand the disease
    Authors: NS Hojo-Souza, DB Pereira, FS de Souza, TA de Oliveir, MS Cardoso, MS Tada, GM Zanini, DC Bartholome, RT Fujiwara, LL Bueno
    Malar. J, 2017;16(1):42.
    Species: Human
    Sample Types: Plasma
  100. A Central Bioactive Region of LTBP-2 Stimulates the Expression of TGF-?1 in Fibroblasts via Akt and p38 Signalling Pathways
    Authors: MA Sideek, J Smith, C Menz, JRJ Adams, AJ Cowin, MA Gibson
    Int J Mol Sci, 2017;18(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  101. Extracellular Hsp90 and TGF? regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model
    Authors: JA de la Mare, T Jurgens, AL Edkins
    BMC Cancer, 2017;17(1):202.
    Species: Human
    Sample Types: Cell Culture Supernates
  102. Composition and functionality of the intrahepatic innate lymphoid cell-compartment in human non-fibrotic and fibrotic livers
    Authors: M Forkel, L Berglin, E Kekäläinen, A Carlsson, E Svedin, J Michaëlsso, M Nagasawa, JS Erjefält, M Mori, M Flodström-, A Bergquist, HG Ljunggren, M Westgren, U Lindforss, D Friberg, C Jorns, E Ellis, NK Björkström, J Mjösberg
    Eur. J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  103. Reduction of relative centrifugal forces increases growth factor release within solid platelet-rich-fibrin (PRF)-based matrices: a proof of concept of LSCC (low speed centrifugation concept)
    Authors: K El Bagdadi, A Kubesch, X Yu, S Al-Maawi, A Orlowska, A Dias, P Booms, E Dohle, R Sader, CJ Kirkpatric, J Choukroun, S Ghanaati
    Eur J Trauma Emerg Surg, 2017;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
  104. Rheumatoid arthritis bone marrow environment supports Th17 response.
    Authors: Ewa Kuca-Warna, Weronika Kurowska, Monika Prochorec, Anna Radzikows, Tomasz Burakowsk, Urszula Skalska, Magdalena Massalska, Magdalena Pleba?czy, Barbara Ma?dyk-No, Iwona S?owi?ska, Robert Gasik, W?odzimierz Ma?li?ski
    Arthritis Research & Therapy, 2017;0(0):1478-6362.
    Species: Human
    Sample Types: Plasma
  105. Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j
    Authors: SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  106. Regulating VEGF signaling in platelet concentrates via specific VEGF sequestering
    Authors: DG Belair, NN Le, WL Murphy
    Biomater Sci, 2016;4(5):819-25.
    Species: Human
    Sample Types: Plasma
  107. The differential expression of protease activated receptors contributes to functional differences between dark and fair keratinocytes
    Authors: Meilang Xue
    J. Dermatol. Sci, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  108. Tumor-Activated Mesenchymal Stromal Cells Promote Osteosarcoma Stemness and Migratory Potential via IL-6 Secretion
    PLoS ONE, 2016;11(11):e0166500.
    Species: Human
    Sample Types: Cell Culture Supernates
  109. Differences in human mesenchymal stem cell secretomes during chondrogenic induction
    Authors: OF Gardner, N Fahy, M Alini, MJ Stoddart
    Eur Cell Mater, 2016;31(0):221-35.
    Species: Human
    Sample Types: Cell Culture Supernates
  110. A novel immunomodulatory function of neutrophils on rhinovirus-activated monocytes in vitro
    Authors: Brian G Oliver
    Thorax, 2016;71(11):1039-1049.
    Species: Human
    Sample Types: Cell Culture Supernates
  111. Lysosomal-associated Transmembrane Protein 4B (LAPTM4B) Decreases Transforming Growth Factor beta1 (TGF-beta1) Production in Human Regulatory T Cells.
    Authors: Huygens C, Lienart S, Dedobbeleer O, Stockis J, Gauthy E, Coulie P, Lucas S
    J Biol Chem, 2015;290(33):20105-16.
    Species: Human
    Sample Types: Cell Culture Supernates
  112. The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
    Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
    J Immunol, 2015;195(5):2429-41.
    Species: Human
    Sample Types: Cell Culture Supernates
  113. Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta.
    Authors: Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El-Sayed K, Ehnert S, Nussler A, Fandrich F, Gieseler F
    PLoS ONE, 2015;10(2):e0118097.
    Species: Human
    Sample Types: Cell Culture Supernates
  114. Human Articular Cartilage Progenitor Cells Are Responsive to Mechanical Stimulation and Adenoviral-Mediated Overexpression of Bone-Morphogenetic Protein 2.
    Authors: Neumann A, Gardner O, Williams R, Alini M, Archer C, Stoddart M
    PLoS ONE, 2015;10(8):e0136229.
    Species: Human
    Sample Types: Cell Culture Supernates
  115. TGF-beta-induced (TGFBI) protein in melanoma: a signature of high metastatic potential.
    Authors: Lauden L, Siewiera J, Boukouaci W, Ramgolam K, Mourah S, Lebbe C, Charron D, Aoudjit F, Jabrane-Ferrat N, Al-Daccak R
    J Invest Dermatol, 2014;134(6):1675-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  116. Asthmatic airway epithelial cells differentially regulate fibroblast expression of extracellular matrix components.
    Authors: Reeves S, Kolstad T, Lien T, Elliott M, Ziegler S, Wight T, Debley J
    J Allergy Clin Immunol, 2014;134(3):663-670.e1.
    Species: Human
    Sample Types: Cell Culture Supernates
  117. Loss of WISP2/CCN5 in estrogen-dependent MCF7 human breast cancer cells promotes a stem-like cell phenotype.
    Authors: Ferrand N, Gnanapragasam A, Dorothee G, Redeuilh G, Larsen A, Sabbah M
    PLoS ONE, 2014;9(2):e87878.
    Species: Human
    Sample Types: Cell Culture Supernates
  118. Activin A as a mediator of NK-dendritic cell functional interactions.
    Authors: Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, Sozzani S
    J Immunol, 2014;192(3):1241-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  119. Antimony resistant Leishmania donovani but not sensitive ones drives greater frequency of potent T-regulatory cells upon interaction with human PBMCs: role of IL-10 and TGF-beta in early immune response.
    Authors: Guha R, Das S, Ghosh J, Sundar S, Dujardin J, Roy S
    PLoS Negl Trop Dis, 2014;8(7):e2995.
    Species: Human
    Sample Types: Cell Culture Supernates
  120. TLR ligands stimulation protects MSC from NK killing.
    Authors: Giuliani M, Bennaceur-Griscelli A, Nanbakhsh A, Oudrhiri N, Chouaib S, Azzarone B, Durrbach A, Lataillade J
    Stem Cells, 2014;32(1):290-300.
    Species: Human
    Sample Types: Cell Culture Supernates
  121. Interaction with colon cancer cells hyperactivates TGF-beta signaling in cancer-associated fibroblasts.
    Authors: Hawinkels L, Paauwe M, Verspaget H, Wiercinska E, van der Zon J, van der Ploeg K, Koelink P, Lindeman J, Mesker W, ten Dijke P, Sier C
    Oncogene, 2014;33(1):97-107.
    Species: Human
    Sample Types: Cell Culture Supernates
  122. Immunomodulatory effects of bone marrow-derived mesenchymal stem cells on pro-inflammatory cytokine-stimulated human corneal epithelial cells.
    Authors: Wen L, Zhu M, Madigan M, You J, King N, Billson F, McClellan K, Sutton G, Petsoglou C
    PLoS ONE, 2014;9(7):e101841.
    Species: Human
    Sample Types: Cell Culture Supernates
  123. Diet-induced increase in plasma oxidized LDL promotes early fibrosis in a renal porcine auto-transplantation model.
    Authors: Chatauret N, Favreau F, Giraud S, Thierry A, Rossard L, Le Pape S, Lerman L, Hauet T
    J Transl Med, 2014;12(0):76.
    Species: Human
    Sample Types: Cell Culture Supernates
  124. Differential chemokine and cytokine production by neonatal bovine gammadelta T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection.
    Authors: McGill J, Nonnecke B, Lippolis J, Reinhardt T, Sacco R
    Immunology, 2013;139(2):227-44.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  125. Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel.
    Authors: Giraldo C, Lopez C, Alvarez M, Samudio I, Prades M, Carmona J
    BMC Vet Res, 2013;9(0):29.
    Species: Equine
    Sample Types: Plasma
  126. Increase of O-glycosylated oncofetal fibronectin in high glucose-induced epithelial-mesenchymal transition of cultured human epithelial cells.
    Authors: Alisson-Silva F, Freire-de-Lima L, Donadio J, Lucena M, Penha L, Sa-Diniz J, Dias W, Todeschini A
    PLoS ONE, 2013;8(4):e60471.
    Species: Human
    Sample Types: Cell Culture Supernates
  127. Human neutrophil peptide-1 (HNP-1): a new anti-leishmanial drug candidate.
    Authors: Dabirian S, Taslimi Y, Zahedifard F, Gholami E, Doustdari F, Motamedirad M, Khatami S, Azadmanesh K, Nylen S, Rafati S
    PLoS Negl Trop Dis, 2013;7(10):e2491.
    Species: Human
    Sample Types: Cell Culture Supernates
  128. Pentraxin 3 (PTX3) expression in allergic asthmatic airways: role in airway smooth muscle migration and chemokine production.
    Authors: Zhang J, Shan L, Koussih L, Redhu NS, Halayko AJ, Chakir J, Gounni AS
    PLoS ONE, 2012;7(4):e34965.
    Species: Human
    Sample Types: Cell Culture Supernates
  129. Elevated adaptive immune responses are associated with latent infections of Wuchereria bancrofti.
    Authors: Arndts K, Deininger S, Specht S, Klarmann U, Mand S, Adjobimey T, Debrah AY, Batsa L, Kwarteng A, Epp C, Taylor M, Adjei O, Layland LE, Hoerauf A
    PLoS Negl Trop Dis, 2012;6(4):e1611.
    Species: Human
    Sample Types: Cell Culture Supernates
  130. Human breast cancer cells enhance self tolerance by promoting evasion from NK cell antitumor immunity.
    Authors: Mamessier E, Sylvain A, Thibult ML, Houvenaeghel G, Jacquemier J, Castellano R, Goncalves A, Andre P, Romagne F, Thibault G, Viens P, Birnbaum D, Bertucci F, Moretta A, Olive D
    J. Clin. Invest., 2011;121(9):3609-22.
    Species: Human
    Sample Types: Cell Culture Supernates
  131. Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques.
    Authors: Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D
    Thromb. Haemost., 2011;107(1):140-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  132. Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
    Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
    FASEB J., 2010;24(10):3782-95.
    Species: Human
    Sample Types: Cell Culture Supernates
  133. Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6.
    Authors: Eikawa S, Ohue Y, Kitaoka K
    J. Immunol., 2010;185(11):6734-40.
    Species: Human
    Sample Types: Pleural Effusion
  134. The roles of Wnt signaling modulators Dickkopf-1 (Dkk1) and Dickkopf-2 (Dkk2) and cell maturation state in osteogenesis on microstructured titanium surfaces.
    Authors: Olivares-Navarrete R, Hyzy S, Wieland M, Boyan BD, Schwartz Z
    Biomaterials, 2010;31(8):2015-24.
    Species: Human
    Sample Types: Cell Lysates
  135. Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy.
    Authors: Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN
    Placenta, 2010;31(6):545-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  136. Anti-programmed cell death 1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W F1 mice.
    Authors: Kasagi S, Kawano S, Okazaki T, Honjo T, Morinobu A, Hatachi S, Shimatani K, Tanaka Y, Minato N, Kumagai S
    J. Immunol., 2010;184(5):2337-47.
    Species: Human
    Sample Types: Serum
  137. Helminth antigens modulate immune responses in cells from multiple sclerosis patients through TLR2-dependent mechanisms.
    Authors: Correale J, Farez M
    J. Immunol., 2009;183(9):5999-6012.
    Species: Human
    Sample Types: Cell Culture Supernates
  138. Growth factor regulation of growth factors in articular chondrocytes.
    Authors: Shi S, Mercer S, Eckert GJ, Trippel SB
    J. Biol. Chem., 2009;284(11):6697-704.
    Species: Human
    Sample Types: Whole Cells
  139. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
    Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
    Blood, 2009;113(13):2955-64.
    Species: Human
    Sample Types: Cell Culture Supernates
  140. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
    Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
    Eur. J. Immunol., 2009;39(5):1221-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  141. Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
    Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V
    J. Exp. Med., 2009;206(4):793-805.
    Species: Human
    Sample Types: Cell Culture Supernates
  142. Hyperexpression of NOD2 in intestinal mast cells of Crohn's disease patients: preferential expression of inflammatory cell-recruiting molecules via NOD2 in mast cells.
    Authors: Okumura S, Yuki K, Kobayashi R, Okamura S, Ohmori K, Saito H, Ra C, Okayama Y
    Clin. Immunol., 2009;130(2):175-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  143. Glioblastoma-secreted factors induce IGFBP7 and angiogenesis by modulating Smad-2-dependent TGF-beta signaling.
    Authors: Pen A, Moreno MJ, Durocher Y, Deb-Rinker P, Stanimirovic DB
    Oncogene, 2008;27(54):6834-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  144. Helminth infections associated with multiple sclerosis induce regulatory B cells.
    Authors: Correale J, Farez M, Razzitte G
    Ann. Neurol., 2008;64(2):187-99.
    Species: Human
    Sample Types: Cell Culture Supernates
  145. Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
    Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
    J. Immunol., 2008;180(7):5109-17.
    Species: Human
    Sample Types: Cell Culture Supernates
  146. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  147. Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2.
    Authors: Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z
    Cancer Res., 2007;67(15):7458-66.
    Species: Human
    Sample Types: Cell Culture Supernates
  148. Transforming growth factor-beta1 suppresses airway hyperresponsiveness in allergic airway disease.
    Authors: Alcorn JF, Rinaldi LM, Jaffe EF, van Loon M, Bates JH, Janssen-Heininger YM, Irvin CG
    Am. J. Respir. Crit. Care Med., 2007;176(10):974-82.
    Species: Mouse
    Sample Types: BALF
  149. Opposite regulation of transforming growth factors-beta2 and -beta3 expression in the human endometrium.
    Authors: Gaide Chevronnay HP, Cornet PB, Delvaux D, Lemoine P, Courtoy PJ, Henriet P, Marbaix E
    Endocrinology, 2007;149(3):1015-25.
    Species: Human
    Sample Types: Tissue Homogenates
  150. Airway remodelling in children with cystic fibrosis.
    Authors: Hilliard TN, Regamey N, Shute JK, Nicholson AG, Alton EW, Bush A, Davies JC
    Thorax, 2007;62(12):1074-80.
    Species: Human
    Sample Types: BALF
  151. Cancer immunoediting by GITR (glucocorticoid-induced TNF-related protein) ligand in humans: NK cell/tumor cell interactions.
    Authors: Baltz KM, Krusch M, Bringmann A, Brossart P, Mayer F, Kloss M, Baessler T, Kumbier I, Peterfi A, Kupka S, Kroeber S, Menzel D, Radsak MP, Rammensee HG, Salih HR
    FASEB J., 2007;21(10):2442-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  152. Coordinated functions of E-cadherin and transforming growth factor beta receptor II in vitro and in vivo.
    Authors: Andl CD, Fargnoli BB, Okawa T, Bowser M, Takaoka M, Nakagawa H, Klein-Szanto A, Hua X, Herlyn M, Rustgi AK
    Cancer Res., 2006;66(20):9878-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  153. Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure.
    Authors: Neurohr C, Nishimura SL, Sheppard D
    Am. J. Respir. Cell Mol. Biol., 2006;35(2):252-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  154. Leukemia inhibitory factor is linked to regulatory transplantation tolerance.
    Authors: Metcalfe SM, Watson TJ, Shurey S, Adams E, Green CJ
    Transplantation, 2005;79(6):726-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  155. Effect of neutralizing transforming growth factor beta1 on the immune response against Mycobacterium tuberculosis in guinea pigs.
    Authors: Allen SS, Cassone L, Lasco TM
    Infect. Immun., 2004;72(3):1358-63.
    Species: Guinea Pig
    Sample Types: Pleural Effusion
  156. The selective estrogen receptor modulator arzoxifene and the rexinoid LG100268 cooperate to promote transforming growth factor beta-dependent apoptosis in breast cancer.
    Authors: Rendi MH, Suh N, Lamph WW, Krajewski S, Reed JC, Heyman RA, Berchuck A, Liby K, Risingsong R, Royce DB, Williams CR, Sporn MB
    Cancer Res., 2004;64(10):3566-71.
    Species: Rat
    Sample Types: Cell Culture Supernates
  157. Presence of dendritic cells, MCP-1, and activated microglia/macrophages in amyotrophic lateral sclerosis spinal cord tissue.
    Authors: Henkel JS, Engelhardt JI, Siklos L, Simpson EP, Kim SH, Pan T, Goodman JC, Siddique T, Beers DR, Appel SH
    Ann. Neurol., 2004;55(2):221-35.
    Species: Human
    Sample Types: CSF
  158. Association of type 2 cytokines with hepatic fibrosis in human Schistosoma mansoni infection.
    Authors: de Jesus AR, Magalhaes A, Miranda DG, Miranda RG, Araujo MI, de Jesus AA, Silva A, Santana LB, Pearce E, Carvalho EM
    Infect. Immun., 2004;72(6):3391-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  159. Coordinate cytokine gene expression in vivo following induction of tuberculous pleurisy in guinea pigs.
    Authors: Allen SS, McMurray DN
    Infect. Immun., 2003;71(8):4271-7.
    Species: Guinea Pig
    Sample Types: Pleural Fluid
  160. Role of TGF-beta1 in relation to exercise-induced type I collagen synthesis in human tendinous tissue.
    Authors: Heinemeier K, Langberg H, Olesen JL, Kjaer M
    J. Appl. Physiol., 2003;95(6):2390-7.
    Species: Human
    Sample Types: Plasma
  161. Effects of a short-course of amoxicillin/clavulanic acid on systemic and mucosal immunity in healthy adult humans.
    Authors: Dufour V, Millon L, Faucher JF, Bard E, Robinet E, Piarroux R, Vuitton DA, Meillet D
    Int. Immunopharmacol., 205;5(5):917-28.
    Species: Human
    Sample Types: Feces
  162. IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation.
    Authors: Kartasheva-Ebertz D, Gaston J, Lair-Mehiri L, Mottez E, Buivan T, Massault P, Scatton O, Gaujoux S, Vaillant J, Pol S, Lagaye S
    Int J Mol Sci, 0;23(17):.
    Species: Human
    Sample Types: Tissue Culture Supernates
  163. Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
    Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
    Mucosal Immunol, 0;5(2):140-9.
    Species: Human
    Sample Types: Cell Culture Supernates

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Reviews for Human TGF-beta 1 DuoSet ELISA

Average Rating: 4.2 (Based on 10 Reviews)

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Human TGF-beta 1 DuoSet ELISA
By Anonymous on 05/12/2021
Sample Tested: Cell culture supernatant
Human TGF-beta 1 DuoSet ELISA DY240
Human TGF-beta 1 DuoSet ELISA
By Anonymous on 03/29/2021
Sample Tested: Serum and Plasma

we used this kit to quantify human TGF beta 1 in human healthy controls sera, it produces a very good standard curve, levels of TGF beta in in healthy controls are very low.

Human TGF-beta 1 DuoSet ELISA DY240
Human TGF-beta 1 DuoSet ELISA
By Anonymous on 11/12/2020
Sample Tested: EDTA Plasma

Very good performance of the kit, we diluted our EDTA plasma samples 1:20 and applied the kit on a 384-well format. Very reliable and replicates are tight.

Human TGF-beta 1 DuoSet ELISA DY240
Human TGF-beta 1 DuoSet ELISA
By Anonymous on 10/29/2019
Sample Tested: Serum-free Cell Culture Supernates

Should mention this kit detects active TGF beta1 only in the description.
Need buy activation kit for total TGF beta1 (including the latent form)

Human TGF-beta 1 DuoSet ELISA DY240
Human TGF-beta 1 DuoSet ELISA
By Tim Stachowski on 08/23/2019
Sample Tested: Purified Standard
Human TGF-beta 1 DuoSet ELISA DY240
Human TGF-beta 1 DuoSet ELISA
By Anonymous on 10/11/2018
Sample Tested: Serum and Plasma
Human TGF-beta 1 DuoSet ELISA
By Maria Yanez on 05/10/2018
Sample Tested: THP-1 human acute monocytic leukemia cell line

I differentiated my THP-1 cells to macrophages and polarized using IL-4 and IL-13. Then I checked the TGF-beta and i have in trouble to detect the concentration for some of my samples. Then I always verify my experiments, and for the other assays I detected tgf-ß

Human TGF-beta 1 DuoSet ELISA DY240
Human TGF-beta 1 DuoSet ELISA
By Anonymous on 05/22/2017
Sample Tested: Adult lung
Human TGF-beta 1 DuoSet ELISA
By Anonymous on 04/03/2017
Sample Tested: Human EDTA plasma
Human TGF-beta 1 DuoSet ELISA DY240
Human TGF-beta 1 DuoSet ELISA
By Anonymous on 07/05/2016
Sample Tested: Human dental periodontal ligament fibroblasts
Human TGF-beta 1 DuoSet ELISA DY240