Human TGF-beta 1 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY240
DY240-05
Ancillary Products Available
Human TGF-beta 1 ELISA Standard Curve
1 Image
Product Details
Procedure
Citations (72)
FAQs
Supplemental Products
Reviews (5)

Human TGF-beta 1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant TGF-ß1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 1 (5 plates): (Catalog # DY007) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 1.

The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4


Reagent Diluent: (Catalog # DY997), or 1.4% delipidized bovine serum, 0.05% Tween 20 in PBS, pH 7.2-7.4, 0.2 μm filtered.

Blocking Buffer: (Catalog # DY004), or 5% Tween 20 in PBS, pH 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Activation Reagents Required:

Sample Activation Kit 1: 3 vials (10 mL/vial) of 1N HCL and 3 vials (10 mL/vial) of 1.2 N NaOH/1M HEPES (R&D Systems, Catalog # DY010).

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 1

Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.

Long Name:
Transforming Growth Factor beta 1
Entrez Gene IDs:
7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
Alternate Names:
CEDLAP; DPD1; latency-associated peptide; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

Assay Procedure

ACTIVATION REAGENT PREPARATION

To activate latent TGF-ß1 to the immunoreactive form, prepare the following solutions for acid activation and neutralization. The solutions may be stored in polypropylene bottles at room temperature for up to one month.

Caution: Wear protective clothing and safety glasses during preparation or use of these reagents. Refer to the appropriate MSDS before use.

1 N HCl (100 mL) - To 91.67 mL of deionized water, slowly add 8.33 mL of 12 N HCl. Mix well.

1.2 N NaOH/0.5 M HEPES (100 mL) - To 75 mL of deionized water, slowly add 12 mL of 10 N NaOH. Mix well. Add 11.9 g of HEPES. Mix well. Bring final volume to 100 mL with deionized water.

TGF-β1 SAMPLE ACTIVATION

To activate latent TGF-β1 to immunoreactive TGF-β1, follow the activation procedure outlined below. Assay samples after neutralization (pH 7.2-7.6). Use polypropylene test tubes.

Note: Do not activate the kit standards. The kit standards contain active recombinant TGF-β1.

Cell Culture Supernates Serum/Plasma
To 100 μL of cell culture supernate, add 20 μL of 1 N HCI. To 40 μL of serum/plasma, add 20 μL of 1 N HCI.
Mix well. Mix well.
Incubate 10 minutes at room temperature. Incubate 10 minutes at room temperature.
Neutralize the acidified sample by adding 20 μL of 1.2 N NaOH/0.5 M HEPES. Neutralize the acidified sample by adding 20 μL of 1.2 N NaOH/0.5 M HEPES.
Mix well. Mix well.
Assay immediately. Prior to the assay, dilute the activated sample 20-fold with Reagent Diluent.*
The concentration read off the standard curve must be multiplied by the dilution factor, 1.4. The concentration read off the standard curve must be multiplied by the appropriate dilution factor, 90.

 

*A suggested 20-fold dilution is 10 μL of activated sample + 190 μL of Reagent Diluent.

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody (to the working concentration stated in the product datasheet ) in PBS without carrier protein. Immediately coat a 96-well microplate with 100 µL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 µL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block each well of the microplate as recommended in the product datasheet. Incubate at room temperature for a minimum of 1 hour.

    Note: The recommended Reagent Diluent typically contains 1% BSA. Some DuoSet Development Kits require alternative blocking agents, or for plates to be blocked overnight with a higher percentage of BSA, please see the product datasheet for details.
     
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

 

PRECAUTION
The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

Assay Procedure

  1. Add 100 µL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 µL of the Detection Antibody, diluted in Reagent Diluent (as recommended in the product datasheet), to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 µL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 µL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 µL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human TGF-beta 1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

72 Citations: Showing 1 - 10
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  1. Comparative Analysis of Different Platelet Lysates and Platelet Rich Preparations to Stimulate Tendon Cell Biology: An In Vitro Study
    Authors: F Klatte-Sch, T Schmidt, M Uckert, S Scheffler, U Kalus, M Rojewski, H Schrezenme, A Pruss, B Wildemann
    Int J Mol Sci, 2018;19(1):.
    Species: Human
    Sample Types: Plasma
  2. Crude leaf extracts of Piperaceae species downmodulate inflammatory responses by human monocytes
    Authors: AC Finato, TF Fraga-Silv, AUC Prati, AA de Souza J, BF Mazzeu, LG Felippe, RA Pinto, MA Golim, MSP Arruda, M Furlan, J Venturini
    PLoS ONE, 2018;13(6):e0198682.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Exosomes Induce Fibroblast Differentiation into Cancer-associated Fibroblasts through TGF? Signaling
    Authors: C R Goulet, G Bernard, S Tremblay, S Chabaud, S Bolduc, F Pouliot
    Mol. Cancer Res., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Butyrate produced by gut commensal bacteria activates TGF-beta1 expression through the transcription factor SP1 in human intestinal epithelial cells
    Authors: C Martin-Gal, F Béguet-Cre, L Marinelli, A Jamet, F Ledue, HM Blottière, N Lapaque
    Sci Rep, 2018;8(1):9742.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. A Central Bioactive Region of LTBP-2 Stimulates the Expression of TGF-?1 in Fibroblasts via Akt and p38 Signalling Pathways
    Authors: MA Sideek, J Smith, C Menz, JRJ Adams, AJ Cowin, MA Gibson
    Int J Mol Sci, 2017;18(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. On the cytokine/chemokine network during Plasmodium vivax malaria: new insights to understand the disease
    Authors: NS Hojo-Souza, DB Pereira, FS de Souza, TA de Oliveir, MS Cardoso, MS Tada, GM Zanini, DC Bartholome, RT Fujiwara, LL Bueno
    Malar. J, 2017;16(1):42.
    Species: Human
    Sample Types: Plasma
  7. In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens
    Authors: N Goli?, K Veljovi?, N Popovi?, J Djoki?, I Strahini?, I Mrvaljevi?, A Terzi?-Vid
    BMC Microbiol., 2017;17(1):108.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Reduction of relative centrifugal forces increases growth factor release within solid platelet-rich-fibrin (PRF)-based matrices: a proof of concept of LSCC (low speed centrifugation concept)
    Authors: K El Bagdadi, A Kubesch, X Yu, S Al-Maawi, A Orlowska, A Dias, P Booms, E Dohle, R Sader, CJ Kirkpatric, J Choukroun, S Ghanaati
    Eur J Trauma Emerg Surg, 2017;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
  9. Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j
    Authors: SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Extracellular Hsp90 and TGF? regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model
    Authors: JA de la Mare, T Jurgens, AL Edkins
    BMC Cancer, 2017;17(1):202.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Activated Tissue-Resident Mesenchymal Stromal Cells Regulate Natural Killer Cell Immune and Tissue-Regenerative Function
    Authors: RM Petri, A Hackel, K Hahnel, CA Dumitru, K Bruderek, SB Flohe, A Paschen, S Lang, S Brandau
    Stem Cell Reports, 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. A nonsense mutation in TLR5 is associated with survival and reduced IL-10 and TNF-? levels in human Melioidosis
    Authors: P Chaichana, N Chantratit, F Brod, S Koosakulni, K Jenjaroen, S Chumseng, M Sumonwiriy, MN Burtnick, PJ Brett, P Teparrukku, D Limmathuro, NPJ Day, SJ Dunachie, TE West
    PLoS Negl Trop Dis, 2017;11(5):e0005587.
    Species: Human
    Sample Types: Plasma
  13. Composition and functionality of the intrahepatic innate lymphoid cell-compartment in human non-fibrotic and fibrotic livers
    Authors: M Forkel, L Berglin, E Kekäläinen, A Carlsson, E Svedin, J Michaëlsso, M Nagasawa, JS Erjefält, M Mori, M Flodström-, A Bergquist, HG Ljunggren, M Westgren, U Lindforss, D Friberg, C Jorns, E Ellis, NK Björkström, J Mjösberg
    Eur. J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Long-term cytokine and growth factor release from equine platelet-rich fibrin clots obtained with two different centrifugation protocols
    Authors: RF Jiménez-Ar, C López, ME Álvarez, C Giraldo, M Prades, JU Carmona
    Cytokine, 2017;97(0):149-155.
    Species: Equine
    Sample Types: Plasma
  15. Neutrophil Extracellular Traps Reprogram IL-4/GM-CSF-Induced Monocyte Differentiation to Anti-inflammatory Macrophages
    Authors: AB Guimarães-, NC Rochael, F Oliveira, J Echevarria, EM Saraiva
    Front Immunol, 2017;8(0):523.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Differences in human mesenchymal stem cell secretomes during chondrogenic induction
    Authors: OF Gardner, N Fahy, M Alini, MJ Stoddart
    Eur Cell Mater, 2016;31(0):221-35.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. The differential expression of protease activated receptors contributes to functional differences between dark and fair keratinocytes
    Authors: Meilang Xue
    J. Dermatol. Sci, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Regulating VEGF signaling in platelet concentrates via specific VEGF sequestering
    Authors: DG Belair, NN Le, WL Murphy
    Biomater Sci, 2016;4(5):819-25.
    Species: Human
    Sample Types: Plasma
  19. A novel immunomodulatory function of neutrophils on rhinovirus-activated monocytes in vitro
    Authors: Brian G Oliver
    Thorax, 2016;71(11):1039-1049.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Tumor-Activated Mesenchymal Stromal Cells Promote Osteosarcoma Stemness and Migratory Potential via IL-6 Secretion
    PLoS ONE, 2016;11(11):e0166500.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Human Articular Cartilage Progenitor Cells Are Responsive to Mechanical Stimulation and Adenoviral-Mediated Overexpression of Bone-Morphogenetic Protein 2.
    Authors: Neumann A, Gardner O, Williams R, Alini M, Archer C, Stoddart M
    PLoS ONE, 2015;10(8):e0136229.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
    Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
    J Immunol, 2015;195(5):2429-41.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Lysosomal-associated Transmembrane Protein 4B (LAPTM4B) Decreases Transforming Growth Factor beta1 (TGF-beta1) Production in Human Regulatory T Cells.
    Authors: Huygens C, Lienart S, Dedobbeleer O, Stockis J, Gauthy E, Coulie P, Lucas S
    J Biol Chem, 2015;290(33):20105-16.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta.
    Authors: Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El-Sayed K, Ehnert S, Nussler A, Fandrich F, Gieseler F
    PLoS ONE, 2015;10(2):e0118097.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. TLR ligands stimulation protects MSC from NK killing.
    Authors: Giuliani M, Bennaceur-Griscelli A, Nanbakhsh A, Oudrhiri N, Chouaib S, Azzarone B, Durrbach A, Lataillade J
    Stem Cells, 2014;32(1):290-300.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Immunomodulatory effects of bone marrow-derived mesenchymal stem cells on pro-inflammatory cytokine-stimulated human corneal epithelial cells.
    Authors: Wen L, Zhu M, Madigan M, You J, King N, Billson F, McClellan K, Sutton G, Petsoglou C
    PLoS ONE, 2014;9(7):e101841.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Diet-induced increase in plasma oxidized LDL promotes early fibrosis in a renal porcine auto-transplantation model.
    Authors: Chatauret N, Favreau F, Giraud S, Thierry A, Rossard L, Le Pape S, Lerman L, Hauet T
    J Transl Med, 2014;12(0):76.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Interaction with colon cancer cells hyperactivates TGF-beta signaling in cancer-associated fibroblasts.
    Authors: Hawinkels L, Paauwe M, Verspaget H, Wiercinska E, van der Zon J, van der Ploeg K, Koelink P, Lindeman J, Mesker W, ten Dijke P, Sier C
    Oncogene, 2014;33(1):97-107.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. TGF-beta-induced (TGFBI) protein in melanoma: a signature of high metastatic potential.
    Authors: Lauden L, Siewiera J, Boukouaci W, Ramgolam K, Mourah S, Lebbe C, Charron D, Aoudjit F, Jabrane-Ferrat N, Al-Daccak R
    J Invest Dermatol, 2014;134(6):1675-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Loss of WISP2/CCN5 in estrogen-dependent MCF7 human breast cancer cells promotes a stem-like cell phenotype.
    Authors: Ferrand N, Gnanapragasam A, Dorothee G, Redeuilh G, Larsen A, Sabbah M
    PLoS ONE, 2014;9(2):e87878.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Antimony resistant Leishmania donovani but not sensitive ones drives greater frequency of potent T-regulatory cells upon interaction with human PBMCs: role of IL-10 and TGF-beta in early immune response.
    Authors: Guha R, Das S, Ghosh J, Sundar S, Dujardin J, Roy S
    PLoS Negl Trop Dis, 2014;8(7):e2995.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Activin A as a mediator of NK-dendritic cell functional interactions.
    Authors: Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, Sozzani S
    J Immunol, 2014;192(3):1241-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Asthmatic airway epithelial cells differentially regulate fibroblast expression of extracellular matrix components.
    Authors: Reeves S, Kolstad T, Lien T, Elliott M, Ziegler S, Wight T, Debley J
    J Allergy Clin Immunol, 2014;134(3):663-670.e1.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Human neutrophil peptide-1 (HNP-1): a new anti-leishmanial drug candidate.
    Authors: Dabirian S, Taslimi Y, Zahedifard F, Gholami E, Doustdari F, Motamedirad M, Khatami S, Azadmanesh K, Nylen S, Rafati S
    PLoS Negl Trop Dis, 2013;7(10):e2491.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Increase of O-glycosylated oncofetal fibronectin in high glucose-induced epithelial-mesenchymal transition of cultured human epithelial cells.
    Authors: Alisson-Silva F, Freire-de-Lima L, Donadio J, Lucena M, Penha L, Sa-Diniz J, Dias W, Todeschini A
    PLoS ONE, 2013;8(4):e60471.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Differential chemokine and cytokine production by neonatal bovine gammadelta T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection.
    Authors: McGill J, Nonnecke B, Lippolis J, Reinhardt T, Sacco R
    Immunology, 2013;139(2):227-44.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  37. Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel.
    Authors: Giraldo C, Lopez C, Alvarez M, Samudio I, Prades M, Carmona J
    BMC Vet Res, 2013;9(0):29.
    Species: Equine
    Sample Types: Plasma
  38. Pentraxin 3 (PTX3) expression in allergic asthmatic airways: role in airway smooth muscle migration and chemokine production.
    Authors: Zhang J, Shan L, Koussih L, Redhu NS, Halayko AJ, Chakir J, Gounni AS
    PLoS ONE, 2012;7(4):e34965.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Elevated adaptive immune responses are associated with latent infections of Wuchereria bancrofti.
    Authors: Arndts K, Deininger S, Specht S, Klarmann U, Mand S, Adjobimey T, Debrah AY, Batsa L, Kwarteng A, Epp C, Taylor M, Adjei O, Layland LE, Hoerauf A
    PLoS Negl Trop Dis, 2012;6(4):e1611.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Human breast cancer cells enhance self tolerance by promoting evasion from NK cell antitumor immunity.
    Authors: Mamessier E, Sylvain A, Thibult ML, Houvenaeghel G, Jacquemier J, Castellano R, Goncalves A, Andre P, Romagne F, Thibault G, Viens P, Birnbaum D, Bertucci F, Moretta A, Olive D
    J. Clin. Invest., 2011;121(9):3609-22.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques.
    Authors: Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D
    Thromb. Haemost., 2011;107(1):140-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. The roles of Wnt signaling modulators Dickkopf-1 (Dkk1) and Dickkopf-2 (Dkk2) and cell maturation state in osteogenesis on microstructured titanium surfaces.
    Authors: Olivares-Navarrete R, Hyzy S, Wieland M, Boyan BD, Schwartz Z
    Biomaterials, 2010;31(8):2015-24.
    Species: Human
    Sample Types: Cell Lysates
  43. Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6.
    Authors: Eikawa S, Ohue Y, Kitaoka K
    J. Immunol., 2010;185(11):6734-40.
    Species: Human
    Sample Types: Pleural Effusion
  44. Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy.
    Authors: Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN
    Placenta, 2010;31(6):545-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Anti-programmed cell death 1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W F1 mice.
    Authors: Kasagi S, Kawano S, Okazaki T, Honjo T, Morinobu A, Hatachi S, Shimatani K, Tanaka Y, Minato N, Kumagai S
    J. Immunol., 2010;184(5):2337-47.
    Species: Human
    Sample Types: Serum
  46. Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
    Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
    FASEB J., 2010;24(10):3782-95.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
    Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V
    J. Exp. Med., 2009;206(4):793-805.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. Hyperexpression of NOD2 in intestinal mast cells of Crohn's disease patients: preferential expression of inflammatory cell-recruiting molecules via NOD2 in mast cells.
    Authors: Okumura S, Yuki K, Kobayashi R, Okamura S, Ohmori K, Saito H, Ra C, Okayama Y
    Clin. Immunol., 2009;130(2):175-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Helminth antigens modulate immune responses in cells from multiple sclerosis patients through TLR2-dependent mechanisms.
    Authors: Correale J, Farez M
    J. Immunol., 2009;183(9):5999-6012.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Growth factor regulation of growth factors in articular chondrocytes.
    Authors: Shi S, Mercer S, Eckert GJ, Trippel SB
    J. Biol. Chem., 2009;284(11):6697-704.
    Species: Human
    Sample Types: Whole Cells
  51. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
    Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
    Eur. J. Immunol., 2009;39(5):1221-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
    Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
    Blood, 2009;113(13):2955-64.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Glioblastoma-secreted factors induce IGFBP7 and angiogenesis by modulating Smad-2-dependent TGF-beta signaling.
    Authors: Pen A, Moreno MJ, Durocher Y, Deb-Rinker P, Stanimirovic DB
    Oncogene, 2008;27(54):6834-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
    Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
    J. Immunol., 2008;180(7):5109-17.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Helminth infections associated with multiple sclerosis induce regulatory B cells.
    Authors: Correale J, Farez M, Razzitte G
    Ann. Neurol., 2008;64(2):187-99.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Airway remodelling in children with cystic fibrosis.
    Authors: Hilliard TN, Regamey N, Shute JK, Nicholson AG, Alton EW, Bush A, Davies JC
    Thorax, 2007;62(12):1074-80.
    Species: Human
    Sample Types: BALF
  57. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. Transforming growth factor-beta1 suppresses airway hyperresponsiveness in allergic airway disease.
    Authors: Alcorn JF, Rinaldi LM, Jaffe EF, van Loon M, Bates JH, Janssen-Heininger YM, Irvin CG
    Am. J. Respir. Crit. Care Med., 2007;176(10):974-82.
    Species: Mouse
    Sample Types: BALF
  59. Opposite regulation of transforming growth factors-beta2 and -beta3 expression in the human endometrium.
    Authors: Gaide Chevronnay HP, Cornet PB, Delvaux D, Lemoine P, Courtoy PJ, Henriet P, Marbaix E
    Endocrinology, 2007;149(3):1015-25.
    Species: Human
    Sample Types: Tissue Homogenates
  60. Cancer immunoediting by GITR (glucocorticoid-induced TNF-related protein) ligand in humans: NK cell/tumor cell interactions.
    Authors: Baltz KM, Krusch M, Bringmann A, Brossart P, Mayer F, Kloss M, Baessler T, Kumbier I, Peterfi A, Kupka S, Kroeber S, Menzel D, Radsak MP, Rammensee HG, Salih HR
    FASEB J., 2007;21(10):2442-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2.
    Authors: Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z
    Cancer Res., 2007;67(15):7458-66.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure.
    Authors: Neurohr C, Nishimura SL, Sheppard D
    Am. J. Respir. Cell Mol. Biol., 2006;35(2):252-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  63. Coordinated functions of E-cadherin and transforming growth factor beta receptor II in vitro and in vivo.
    Authors: Andl CD, Fargnoli BB, Okawa T, Bowser M, Takaoka M, Nakagawa H, Klein-Szanto A, Hua X, Herlyn M, Rustgi AK
    Cancer Res., 2006;66(20):9878-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. Leukemia inhibitory factor is linked to regulatory transplantation tolerance.
    Authors: Metcalfe SM, Watson TJ, Shurey S, Adams E, Green CJ
    Transplantation, 2005;79(6):726-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  65. The selective estrogen receptor modulator arzoxifene and the rexinoid LG100268 cooperate to promote transforming growth factor beta-dependent apoptosis in breast cancer.
    Authors: Rendi MH, Suh N, Lamph WW, Krajewski S, Reed JC, Heyman RA, Berchuck A, Liby K, Risingsong R, Royce DB, Williams CR, Sporn MB
    Cancer Res., 2004;64(10):3566-71.
    Species: Rat
    Sample Types: Cell Culture Supernates
  66. Effect of neutralizing transforming growth factor beta1 on the immune response against Mycobacterium tuberculosis in guinea pigs.
    Authors: Allen SS, Cassone L, Lasco TM
    Infect. Immun., 2004;72(3):1358-63.
    Species: Guinea Pig
    Sample Types: Pleural Effusion
  67. Association of type 2 cytokines with hepatic fibrosis in human Schistosoma mansoni infection.
    Authors: de Jesus AR, Magalhaes A, Miranda DG, Miranda RG, Araujo MI, de Jesus AA, Silva A, Santana LB, Pearce E, Carvalho EM
    Infect. Immun., 2004;72(6):3391-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. Presence of dendritic cells, MCP-1, and activated microglia/macrophages in amyotrophic lateral sclerosis spinal cord tissue.
    Authors: Henkel JS, Engelhardt JI, Siklos L, Simpson EP, Kim SH, Pan T, Goodman JC, Siddique T, Beers DR, Appel SH
    Ann. Neurol., 2004;55(2):221-35.
    Species: Human
    Sample Types: CSF
  69. Role of TGF-beta1 in relation to exercise-induced type I collagen synthesis in human tendinous tissue.
    Authors: Heinemeier K, Langberg H, Olesen JL, Kjaer M
    J. Appl. Physiol., 2003;95(6):2390-7.
    Species: Human
    Sample Types: Plasma
  70. Coordinate cytokine gene expression in vivo following induction of tuberculous pleurisy in guinea pigs.
    Authors: Allen SS, McMurray DN
    Infect. Immun., 2003;71(8):4271-7.
    Species: Guinea Pig
    Sample Types: Pleural Fluid
  71. Effects of a short-course of amoxicillin/clavulanic acid on systemic and mucosal immunity in healthy adult humans.
    Authors: Dufour V, Millon L, Faucher JF, Bard E, Robinet E, Piarroux R, Vuitton DA, Meillet D
    Int. Immunopharmacol., 205;5(5):917-28.
    Species: Human
    Sample Types: Feces
  72. Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
    Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
    Mucosal Immunol, 0;5(2):140-9.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human TGF-beta 1 DuoSet ELISA
By Anonymous on 05/10/2018
Sample Tested: THP-1 human acute monocytic leukemia cell line

I differentiated my THP-1 cells to macrophages and polarized using IL-4 and IL-13. Then I checked the TGF-beta and i have in trouble to detect the concentration for some of my samples. Then I always verify my experiments, and for the other assays I detected tgf-beta


Human TGF-beta 1 DuoSet ELISA
By Anonymous on 07/17/2017
Sample Tested: THP-1 human acute monocytic leukemia cell line

Human TGF-beta 1 DuoSet ELISA
By Anonymous on 05/22/2017
Sample Tested: Adult lung

Human TGF-beta 1 DuoSet ELISA
By Anonymous on 04/03/2017
Sample Tested: Human EDTA plasma

Human TGF-beta 1 DuoSet ELISA
By Anonymous on 07/05/2016
Sample Tested: Human dental periodontal ligament fibroblasts