Human TGF-beta 2 Quantikine ELISA Kit

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Human TGF-beta 2 ELISA Standard Curve
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Citations (39)
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Human TGF-beta 2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (125 uL), Serum (125 uL), EDTA Plasma (125 uL), Heparin Plasma (125 uL), Citrate Plasma (125 uL)
Sensitivity
7 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human TGF-beta 2 and natural porcine TGF-beta 2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human TGF-beta 2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure activated TGF-beta 2 in cell culture supernates, serum, and plasma. The Quantikine Human TGF-beta 2 Immunoassay contains CHO cell-derived recombinant human TGF-beta 2 and antibodies raised against the recombinant protein. The assay has been shown to quantitate the recombinant factor accurately. Results obtained using human TGF-beta 2 showed linear curves that were parallel to the standard curves obtained using the recombinant TGF-beta 2 kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TGF-beta 2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 194 509 1044 196 500 1015
Standard Deviation 13.8 27 28.3 9.8 21.5 43.6
CV% 7.1 5.3 2.7 5 4.3 4.3

Recovery

To assess recovery of the assay, samples of various matrices were spiked with three concentrations of TGF-beta 2, activated, and measured in the assay.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 101 89-113
Citrate Plasma (n=5) 107 93-116
EDTA Plasma (n=5) 101 95-111
Heparin Plasma (n=5) 102 89-113
Serum (n=5) 86 73-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of TGF-beta 2, activated and then serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TGF-beta 2 ELISA Linearity

Data Examples

Human TGF-beta 2 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 2

Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.

Long Name:
Transforming Growth Factor beta 2
Entrez Gene IDs:
7042 (Human); 21808 (Mouse); 397084 (Porcine)
Alternate Names:
BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; MGC116892; polyergin; TGFB2; TGFbeta 2; TGF-beta 2; TGF-beta2; TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of the appropriate Assay Diluent to each well.

  5. 100 µL Standard, Control, or Activated Sample
  6.   Add 100 µL of Standard, control, or activated sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 3 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human TGF-beta 2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Involvement of the ubiquitin-proteasome system in the expression of extracellular matrix genes in retinal pigment epithelial cells
    Authors: JE Ramos de C, MT Verwoert, IMC Vogels, EA Reits, CJF Van Noorde, I Klaassen, RO Schlingema
    Biochem Biophys Rep, 2018;13(0):83-92.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. TGF-? concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration
    Authors: GM Tosi, G Neri, E Caldi, F Fusco, T Bacci, A Tarantello, E Nuti, D Marigliani, S Baiocchi, C Traversi, M Barbarino, CM Eandi, B Parolini, L Mundo, A Santucci, M Orlandini, F Galvagni
    Sci Rep, 2018;8(1):8053.
    Species: Human
    Sample Types: Aqueous Humor
  3. Transforming growth factor ?2 (TGF?2) signaling plays a key role in glucocorticoid-induced ocular hypertension
    Authors: RB Kasetti, P Maddineni, P Patel, C Searby, VC Sheffield, GS Zode
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. MicroRNA-30b controls endothelial cell capillary morphogenesis through regulation of transforming growth factor beta 2
    Authors: GA Howe, K Kazda, CL Addison
    PLoS ONE, 2017;12(10):e0185619.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. MicroRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression
    Authors: Q Cao, F Liu, K Ji, N Liu, Y He, W Zhang, L Wang
    J. Exp. Clin. Cancer Res, 2017;36(1):29.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. GDF15 is elevated in mice following retinal ganglion cell death and in glaucoma patients
    Authors: N Ban, CJ Siegfried, JB Lin, YB Shui, J Sein, W Pita-Thoma, A Sene, A Santeford, M Gordon, R Lamb, Z Dong, SC Kelly, V Cavalli, J Yoshino, RS Apte
    JCI Insight, 2017;2(9):.
    Species: Human
    Sample Types: Aqueous Humor
  7. Active transforming growth factor-?2 in the aqueous humor of posterior polymorphous corneal dystrophy patients
    Authors: A Stadnikova, L Dudakova, P Skalicka, Z Valenta, M Filipec, K Jirsova
    PLoS ONE, 2017;12(4):e0175509.
    Species: Human
    Sample Types: Aqueous Humor
  8. Exosomes in Human Breast Milk Promote EMT
    Authors: W Qin, Y Tsukasaki, S Dasgupta, N Mukhopadhy, M Ikebe, ER Sauter
    Clin Cancer Res, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  9. Fibroblast growth factor (FGF) signaling regulates transforming growth factor beta (TGF?)-dependent smooth muscle cell phenotype modulation
    Sci Rep, 2016;6(0):33407.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Remyelination Is Correlated with Regulatory T Cell Induction Following Human Embryoid Body-Derived Neural Precursor Cell Transplantation in a Viral Model of Multiple Sclerosis
    Authors: Warren C Plaisted
    PLoS ONE, 2016;11(6):e0157620.
    Species: Human
    Sample Types: Whole Cells
  11. An Inducible TGF-beta2-TGFbetaR Pathway Modulates the Sensitivity of HNSCC Cells to Tyrosine Kinase Inhibitors Targeting Dominant Receptor Tyrosine Kinases.
    Authors: Kleczko E, Kim J, Keysar S, Heasley L, Eagles J, Simon M, Marshall M, Singleton K, Jimeno A, Tan A, Heasley L
    PLoS ONE, 2015;10(5):e0123600.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Differential knockdown of TGF-beta ligands in a three-dimensional co-culture tumor- stromal interaction model of lung cancer.
    Authors: Horie M, Saito A, Noguchi S, Yamaguchi Y, Ohshima M, Morishita Y, Suzuki H, Kohyama T, Nagase T
    BMC Cancer, 2014;14(0):580.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Time-dependent expression profiles of microRNAs and mRNAs in rat milk whey.
    Authors: Izumi, Hirohisa, Kosaka, Nobuyosh, Shimizu, Takashi, Sekine, Kazunori, Ochiya, Takahiro, Takase, Mitsunor
    PLoS ONE, 2014;9(2):e88843.
    Species: Rat
    Sample Types: Serum
  14. Vitamin D favorably alters the cancer promoting prostaglandin cascade.
    Authors: Qin W, Smith C, Jensen M, Holick M, Sauter E
    Anticancer Res, 2013;33(9):3861-6.
    Species: Human
    Sample Types: Serum
  15. TGF-beta signaling regulates neuronal C1q expression and developmental synaptic refinement.
    Authors: Bialas A, Stevens B
    Nat Neurosci, 2013;16(12):1773-82.
    Species: Rat
    Sample Types: Cell Culture Supernates
  16. The systemic cytokine environment is permanently altered in multiple myeloma.
    Authors: Zheng M, Zhang Z, Bemis K, Belch A, Pilarski L, Shively J, Kirshner J
    PLoS ONE, 2013;8(3):e58504.
    Species: Human
    Sample Types: Plasma
  17. Differential expression of cancer associated proteins in breast milk based on age at first full term pregnancy.
    Authors: Qin W, Zhang K, Kliethermes B, Ruhlen RL, Browne EP, Arcaro KF, Sauter ER
    BMC Cancer, 2012;12(0):100.
    Species: Human
    Sample Types: Milk
  18. An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition.
    Authors: Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim YY, Lindeman GJ, Shannon MF, Drew PA, Khew-Goodall Y, Goodall GJ
    Mol. Biol. Cell, 2011;22(10):1686-98.
    Species: Canine
    Sample Types: Cell Culture Supernates
  19. Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
    Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
    J. Bone Miner. Res., 2011;26(8):1891-903.
    Species: Human
    Sample Types: Serum
  20. Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring.
    Authors: Holmlund U, Amoudruz P, Johansson MA, Haileselassie Y, Ongoiba A, Kayentao K, Traore B, Doumbo S, Schollin J, Doumbo O, Montgomery SM, Sverremark-Ekstrom E
    Clin. Exp. Immunol., 2010;162(3):500-9.
    Species: Human
    Sample Types: Milk
  21. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
    Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
    Transplantation, 2009;88(9):1109-16.
    Species: Human
    Sample Types: Urine
  22. Potent inhibition of cicatricial contraction in proliferative vitreoretinal diseases by statins.
    Authors: Kawahara S, Hata Y, Kita T, Arita R, Miura M, Nakao S, Mochizuki Y, Enaida H, Kagimoto T, Goto Y, Hafezi-Moghadam A, Ishibashi T
    Diabetes, 2008;57(10):2784-93.
    Species: Human
    Sample Types: Vitreous Humor
  23. Connective tissue growth factor as a mediator of intraocular fibrosis.
    Authors: He S, Chen Y, Khankan R, Barron E, Burton R, Zhu D, Ryan SJ, Oliver N, Hinton DR
    Invest. Ophthalmol. Vis. Sci., 2008;49(9):4078-88.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Connexin43 knockdown accelerates wound healing but inhibits mesenchymal transition after corneal endothelial injury in vivo.
    Authors: Nakano Y, Oyamada M, Dai P, Nakagami T, Kinoshita S, Takamatsu T
    Invest. Ophthalmol. Vis. Sci., 2008;49(1):93-104.
    Species: Rat
    Sample Types: Aqueous Humor
  25. Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
    Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
    AIDS Res. Hum. Retroviruses, 2007;23(7):886-90.
    Species: Human
    Sample Types: Plasma
  26. Interferon-gamma differentially regulates TGF-beta1 and TGF-beta2 expression in human retinal pigment epithelial cells through JAK-STAT pathway.
    Authors: Nagineni CN, Cherukuri KS, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2007;210(1):192-200.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Tumor-derived TGFbeta-1 induces dendritic cell apoptosis in the sentinel lymph node.
    Authors: Ito M, Minamiya Y, Kawai H, Saito S, Saito H, Nakagawa T, Imai K, Hirokawa M, Ogawa J
    J. Immunol., 2006;176(9):5637-43.
    Species: Human
    Sample Types: Tissue Homogenates
  28. Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
    Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
    Transpl. Int., 2005;18(2):177-85.
    Species: Human
    Sample Types: Plasma
  29. Role of transforming growth factor-beta in breast milk for initiation of IgA production in newborn infants.
    Authors: Ogawa J, Sasahara A, Yoshida T, Sira MM, Futatani T, Kanegane H, Miyawaki T
    Early Hum. Dev., 2004;77(1):67-75.
    Species: Human
    Sample Types: Colostrum
  30. T cell-specific ablation of Fas leads to Fas ligand-mediated lymphocyte depletion and inflammatory pulmonary fibrosis.
    Authors: Hao Z, Hampel B, Yagita H, Rajewsky K
    J. Exp. Med., 2004;199(10):1355-65.
    Species: Mouse
    Sample Types: Serum
  31. Expression of connective tissue growth factor after glaucoma filtration surgery in a rabbit model.
    Authors: Esson DW, Neelakantan A, Iyer SA, Blalock TD, Balasubramanian L, Grotendorst GR, Schultz GS, Sherwood MB
    Invest. Ophthalmol. Vis. Sci., 2004;45(2):485-91.
    Species: Rabbit
    Sample Types: Tissue Homogenates
  32. Effect of ozone exposure on airway responses to inhaled allergen in asthmatic subjects.
    Authors: Chen LL, Tager IB, Peden DB, Christian DL, Ferrando RE, Welch BS, Balmes JR
    Chest, 2004;125(6):2328-35.
    Species: Human
    Sample Types: BALF
  33. Gene transfer of soluble TGF-beta type II receptor inhibits experimental proliferative vitreoretinopathy.
    Authors: Oshima Y, Sakamoto T, Hisatomi T, Tsutsumi C, Ueno H, Ishibashi T
    Gene Ther., 2002;9(18):1214-20.
    Species: Rabbit
    Sample Types: Vitreous Humor
  34. Progesterone upregulates TGF-b isoforms (b1, b2, and b3) expression in normal human osteoblast-like cells.
    Authors: Luo XH, Liao EY, Su X
    Calcif. Tissue Int., 2002;71(4):329-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Circadian variations of transforming growth factor-beta2 and basic fibroblast growth factor in the rabbit aqueous humor.
    Authors: Liu JH
    Curr. Eye Res., 2002;24(1):75-80.
    Species: Rabbit
    Sample Types: Aqueous Humor
  36. Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle.
    Authors: Bracamonte MP, Rud KS, Owen WG, Miller VM
    Am. J. Physiol. Heart Circ. Physiol., 2002;283(3):H853-60.
    Species: Porcine
    Sample Types: Cell Lysates
  37. Transforming growth factor-beta -Smad signaling pathway cooperates with NF-kappa B to mediate nontypeable Haemophilus influenzae-induced MUC2 mucin transcription.
    Authors: Jono H, Shuto T, Xu H, Kai H, Lim DJ, Gum JR, Kim YS, Yamaoka S, Feng XH, Li JD
    J. Biol. Chem., 2002;277(47):45547-57.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
    Authors: Truckenmiller ME, Vawter MP, Zhang P, Conejero-Goldberg C, Dillon-Carter O, Morales N, Cheadle C, Becker KG, Freed WJ
    Exp. Neurol., 2002;175(0):318.
    Species: Rat
    Sample Types: Cell Culture Supernates
  39. Transforming growth factor-beta in breast milk: a potential regulator of atopic disease at an early age.
    Authors: Kalliomaki M, Ouwehand A, Arvilommi H, Kero P, Isolauri E
    J. Allergy Clin. Immunol., 1999;104(6):1251-7.
    Species: Human
    Sample Types: Milk

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Human TGF-beta 2 Quantikine ELISA Kit
By Anonymous on 02/04/2017
Sample Tested: Cell Culture Supernates