Human TGF-beta 2 Quantikine ELISA Kit

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PDB250
SB250
DB250
Control Products Available
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Human TGF-beta 2 ELISA Standard Curve
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Product Details
Procedure
Citations (56)
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Human TGF-beta 2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (125 uL), Serum (125 uL), EDTA Plasma (125 uL), Heparin Plasma (125 uL), Citrate Plasma (125 uL)
Sensitivity
7 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human TGF-beta 2 and natural porcine TGF-beta 2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human TGF-beta 2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure activated TGF-beta 2 in cell culture supernates, serum, and plasma. The Quantikine Human TGF-beta 2 Immunoassay contains CHO cell-derived recombinant human TGF-beta 2 and antibodies raised against the recombinant protein. The assay has been shown to quantitate the recombinant factor accurately. Results obtained using human TGF-beta 2 showed linear curves that were parallel to the standard curves obtained using the recombinant TGF-beta 2 kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TGF-beta 2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 194 509 1044 196 500 1015
Standard Deviation 13.8 27 28.3 9.8 21.5 43.6
CV% 7.1 5.3 2.7 5 4.3 4.3

Recovery

To assess recovery of the assay, samples of various matrices were spiked with three concentrations of TGF-beta 2, activated, and measured in the assay.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 101 89-113
Citrate Plasma (n=5) 107 93-116
EDTA Plasma (n=5) 101 95-111
Heparin Plasma (n=5) 102 89-113
Serum (n=5) 86 73-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of TGF-beta 2, activated and then serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TGF-beta 2 ELISA Linearity

Data Examples

Human TGF-beta 2 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 2

TGF-beta 2 is synthesized as a prepro-cytokine with a 19 amino acid (aa) signal sequence, a 283 aa pro-region, and a 112 aa mature segment (1-5). It dimerizes with formation of disulfide bonds between the 'pro' regions and disulfide bonds between the 'mature' regions. The mature region is 71% and 80% identical with human TGF-beta 1 and TGF-beta 3 (6, 7) and 97% identical with the corresponding mouse protein (8). After proteolytic cleavage of the disulfide-linked mature region, it remains hydrogen-bonded to the disulfide-linked prosegments (LAP or latencyassociated protein) (1, 2, 9). If secreted in this form, LAP keeps TGF-beta 2 in an inactive state until dissociation, caused by proteases, glycosidases, or extreme pH (2, 9). In many types of cells, an additional protein, latent TGF-beta binding protein (LTBP), is covalently linked to the LAP homodimer prior to secretion. LTBP, a 130 kDa cysteine-rich glycoprotein, creates a 235 kDa large latent complex that is secreted, most likely binding to the extracellular matrix (1, 9-11). The latency components are believed to act as natural antagonists of TGF-beta activity, to target TGF-beta to distinct tissues, and to maintain a reservoir of TGF-beta (1, 2, 12). On release from latency, active homodimeric TGF-beta can bind to cell-surface receptors or to other proteins, such as alpha 2-macroglobulin (13). 

The signal transducing receptor for TGF-beta 2 is a heterotetrameric complex of two type I signal-transducing receptors (53 kDa; TGF-beta RI) and two type II ligand-binding receptors (75-85 kDa; TGF-beta RII) (14-19). The binding of TGF-beta 2 appears to initially involve a type III TGF-beta receptor, either 300 kDa betaglycan (20) or 180 kDa endoglin (14, 21), which then "hands off" to TGF-beta RII. 
TGF-beta 2 is expressed by a variety of cells, including osteoclasts, thymic epithelium, keratinocytes, hepatocytes, chief cells of the stomach, satellite cells, skeletal muscle cells, prostatic epithelium, bronchial epithelium, neurons and astrocytes, fibroblasts and visceral smooth muscle, and macrophages (14-19). TGF-beta 2 has marked cross-species bioactivity (e.g., human TGF-beta 2 is active on mouse cells (33), while porcine TGF-beta 2 is active on rabbit cells (34)). TGF-beta 2 has four fundamental activities: it is a growth inhibitor for most types of cells; it enhances the deposition of extracellular matrix; it is immunosuppressive, suppressing APC expression of both IL-12 and CD40L while upregulating IL-10 secretion; and during fetal development, it is expressed in discrete areas, such as epithelium, myocardium, cartilage and bone of extremities and in the nervous system, suggesting specific functions (1, 35-37).

Long Name:
Transforming Growth Factor beta 2
Entrez Gene IDs:
7042 (Human); 21808 (Mouse); 397084 (Porcine)
Alternate Names:
BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; MGC116892; polyergin; TGFB2; TGFbeta 2; TGF-beta 2; TGF-beta2; TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of the appropriate Assay Diluent to each well.

  5. 100 µL Standard, Control, or Activated Sample
  6.   Add 100 µL of Standard, control, or activated sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 3 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human TGF-beta 2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

56 Citations: Showing 1 - 10
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  1. Maternal breast milk microbiota and immune markers in relation to subsequent development of celiac disease in offspring
    Authors: J Štšepetova, K Simre, A Tagoma, O Uibo, A Peet, H Siljander, V Tillmann, M Knip, R Mändar, R Uibo
    Scientific Reports, 2022;12(1):6607.
    Species: Human
    Sample Types: Breast Milk
  2. Oxidative Stress Enhances the TGF-beta2-RhoA-MRTF-A/B Axis in Cells Entering Endothelial-Mesenchymal Transition
    Authors: K Sobierajsk, ME Wawro, J Niewiarows
    International Journal of Molecular Sciences, 2022;23(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Role of epidermal growth factor receptor inhibitor-induced interferon pathway signaling in the head and neck squamous cell carcinoma therapeutic response
    Authors: SP Korpela, TK Hinz, A Oweida, J Kim, J Calhoun, R Ferris, RA Nemenoff, SD Karam, ET Clambey, LE Heasley
    Journal of Translational Medicine, 2021;19(1):43.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Desmoglein-2 harnesses a PDZ-GEF2/Rap1 signaling axis to control cell spreading and focal adhesions independent of cell-cell adhesion
    Authors: WT Shelton, SM Thomas, HR Alexander, CE Thomes, DE Conway, AD Dubash
    Scientific Reports, 2021;11(1):13295.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. A static magnetic field changes the expression profile of the transforming growth factor &beta family genes in human cells that have been treated with fluoride ions
    Authors: M Kimsa-Dude, A Synowiec-W, A Krawczyk, C Kruszniews, J Gola
    Cytokine, 2021;0(0):155537.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. TGFbeta2-induced formation of lipid droplets supports acidosis-driven EMT and the metastatic spreading of cancer cells
    Authors: C Corbet, E Bastien, JP Santiago d, E Dierge, R Martherus, C Vander Lin, B Doix, C Degavre, C Guilbaud, L Petit, C Michiels, C Dessy, Y Larondelle, O Feron
    Nat Commun, 2020;11(1):454.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Novel approach to visualize the inter-dependencies between maternal sensitization, breast milk immune components and human milk oligosaccharides in the LIFE Child cohort
    Authors: L Michel, M Shevlyakov, E Ní Cléirig, E Eckhardt, S Holvoet, S Nutten, N Sprenger, A Körner, M Vogel, C Nembrini, W Kiess, C Blanchard
    PLoS ONE, 2020;15(4):e0230472.
    Species: Human
    Sample Types: Breast Milk
  8. Age-induced accumulation of methylmalonic acid promotes tumour progression
    Authors: AP Gomes, D Ilter, V Low, JE Endress, J Fernández-, A Rosenzweig, T Schild, D Broekaert, A Ahmed, M Planque, I Elia, J Han, C Kinzig, E Mullarky, AP Mutvei, J Asara, R de Cabo, LC Cantley, N Dephoure, SM Fendt, J Blenis
    Nature, 2020;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Dynamics of Early Signalling Events during Fracture Healing and Potential Serum Biomarkers of Fracture Non-Union in Humans
    Authors: AN Burska, PV Giannoudis, BH Tan, D Ilas, E Jones, F Ponchel
    J Clin Med, 2020;9(2):.
    Species: Human
    Sample Types: Serum
  10. Does Caesarean Section or Preterm Delivery Influence TGF-&beta2 Concentrations in Human Colostrum?
    Authors: B Kociszewsk, E Sibanda, DM Radomska-L, K Taradaj, P Kocio?ek, T Ginda, M Gruszfeld, E Jankowska-, B Pietrzak, M Wielgo?, J Malejczyk
    Nutrients, 2020;12(4):.
    Species: Human
    Sample Types: Cell Culture Super
  11. Acidosis-Induced TGF-beta2 Production Promotes Lipid Droplet Formation in Dendritic Cells and Alters Their Potential to Support Anti-Mesothelioma T Cell Response
    Authors: N Trempolec, C Degavre, B Doix, D Brusa, C Corbet, O Feron
    Cancers (Basel), 2020;12(5):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Age-induced accumulation of methylmalonic acid promotes tumour progression
    Authors: AP Gomes, D Ilter, V Low, JE Endress, J Fernández-, A Rosenzweig, T Schild, D Broekaert, A Ahmed, M Planque, I Elia, J Han, C Kinzig, E Mullarky, AP Mutvei, J Asara, R de Cabo, LC Cantley, N Dephoure, SM Fendt, J Blenis
    Nature, 2020;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA
    Authors: YY Liu, SY Wang, YN Li, WJ Bian, LQ Zhang, YH Li, L Long, X Liu, XW Zhang, ZG Li
    Chin. Med. J., 2020;0(6):679-686.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles
    Authors: G Merckx, B Hosseinkha, S Kuypers, S Deville, J Irobi, I Nelissen, L Michiels, I Lambrichts, A Bronckaers
    Cells, 2020;9(2):.
    Species: Human
    Sample Types: Serum
  15. MicroRNA-411 and Its 5'-IsomiR Have Distinct Targets and Functions and Are Differentially Regulated in the Vasculature under Ischemia
    Authors: RVCT van der Kw, T Woudenberg, PHA Quax, AY Nossent
    Mol. Ther., 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Oral Tolerance Induction to Newly Introduced Allergen is Favored by a Transforming Growth Factor-beta-Enriched Formula
    Authors: S Holvoet, M Perrot, N de Groot, G Prioult, T Mikogami, V Verhasselt, S Nutten
    Nutrients, 2019;11(9):.
    Species: Bovine
    Sample Types: Milk Whey
  17. KLF4 prevents epithelial to mesenchymal transition in human corneal epithelial cells via endogenous TGF- β2 suppression
    Authors: S Fujimoto, R Hayashi, S Hara, Y Sasamoto, J Harrington, M Tsujikawa, K Nishida
    Regen Ther, 2019;11(0):249-257.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. TGF-? concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration
    Authors: GM Tosi, G Neri, E Caldi, F Fusco, T Bacci, A Tarantello, E Nuti, D Marigliani, S Baiocchi, C Traversi, M Barbarino, CM Eandi, B Parolini, L Mundo, A Santucci, M Orlandini, F Galvagni
    Sci Rep, 2018;8(1):8053.
    Species: Human
    Sample Types: Aqueous Humor
  19. Involvement of the ubiquitin-proteasome system in the expression of extracellular matrix genes in retinal pigment epithelial cells
    Authors: JE Ramos de C, MT Verwoert, IMC Vogels, EA Reits, CJF Van Noorde, I Klaassen, RO Schlingema
    Biochem Biophys Rep, 2018;13(0):83-92.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Transforming growth factor ?2 (TGF?2) signaling plays a key role in glucocorticoid-induced ocular hypertension
    Authors: RB Kasetti, P Maddineni, P Patel, C Searby, VC Sheffield, GS Zode
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. GDF15 is elevated in mice following retinal ganglion cell death and in glaucoma patients
    Authors: N Ban, CJ Siegfried, JB Lin, YB Shui, J Sein, W Pita-Thoma, A Sene, A Santeford, M Gordon, R Lamb, Z Dong, SC Kelly, V Cavalli, J Yoshino, RS Apte
    JCI Insight, 2017;2(9):.
    Species: Human
    Sample Types: Aqueous Humor
  22. Active transforming growth factor-?2 in the aqueous humor of posterior polymorphous corneal dystrophy patients
    Authors: A Stadnikova, L Dudakova, P Skalicka, Z Valenta, M Filipec, K Jirsova
    PLoS ONE, 2017;12(4):e0175509.
    Species: Human
    Sample Types: Aqueous Humor
  23. MicroRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression
    Authors: Q Cao, F Liu, K Ji, N Liu, Y He, W Zhang, L Wang
    J. Exp. Clin. Cancer Res, 2017;36(1):29.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. MicroRNA-30b controls endothelial cell capillary morphogenesis through regulation of transforming growth factor beta 2
    Authors: GA Howe, K Kazda, CL Addison
    PLoS ONE, 2017;12(10):e0185619.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Remyelination Is Correlated with Regulatory T Cell Induction Following Human Embryoid Body-Derived Neural Precursor Cell Transplantation in a Viral Model of Multiple Sclerosis
    Authors: Warren C Plaisted
    PLoS ONE, 2016;11(6):e0157620.
    Species: Human
    Sample Types: Whole Cells
  26. Exosomes in Human Breast Milk Promote EMT
    Authors: W Qin, Y Tsukasaki, S Dasgupta, N Mukhopadhy, M Ikebe, ER Sauter
    Clin Cancer Res, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  27. Fibroblast growth factor (FGF) signaling regulates transforming growth factor beta (TGF?)-dependent smooth muscle cell phenotype modulation
    Sci Rep, 2016;6(0):33407.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. An Inducible TGF-beta2-TGFbetaR Pathway Modulates the Sensitivity of HNSCC Cells to Tyrosine Kinase Inhibitors Targeting Dominant Receptor Tyrosine Kinases.
    Authors: Kleczko E, Kim J, Keysar S, Heasley L, Eagles J, Simon M, Marshall M, Singleton K, Jimeno A, Tan A, Heasley L
    PLoS ONE, 2015;10(5):e0123600.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Time-dependent expression profiles of microRNAs and mRNAs in rat milk whey.
    Authors: Izumi, Hirohisa, Kosaka, Nobuyosh, Shimizu, Takashi, Sekine, Kazunori, Ochiya, Takahiro, Takase, Mitsunor
    PLoS ONE, 2014;9(2):e88843.
    Species: Rat
    Sample Types: Serum
  30. Differential knockdown of TGF-beta ligands in a three-dimensional co-culture tumor- stromal interaction model of lung cancer.
    Authors: Horie M, Saito A, Noguchi S, Yamaguchi Y, Ohshima M, Morishita Y, Suzuki H, Kohyama T, Nagase T
    BMC Cancer, 2014;14(0):580.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Vitamin D favorably alters the cancer promoting prostaglandin cascade.
    Authors: Qin W, Smith C, Jensen M, Holick M, Sauter E
    Anticancer Res, 2013;33(9):3861-6.
    Species: Human
    Sample Types: Serum
  32. The systemic cytokine environment is permanently altered in multiple myeloma.
    Authors: Zheng M, Zhang Z, Bemis K, Belch A, Pilarski L, Shively J, Kirshner J
    PLoS ONE, 2013;8(3):e58504.
    Species: Human
    Sample Types: Plasma
  33. TGF-beta signaling regulates neuronal C1q expression and developmental synaptic refinement.
    Authors: Bialas A, Stevens B
    Nat Neurosci, 2013;16(12):1773-82.
    Species: Rat
    Sample Types: Cell Culture Supernates
  34. Differential expression of cancer associated proteins in breast milk based on age at first full term pregnancy.
    Authors: Qin W, Zhang K, Kliethermes B, Ruhlen RL, Browne EP, Arcaro KF, Sauter ER
    BMC Cancer, 2012;12(0):100.
    Species: Human
    Sample Types: Milk
  35. An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition.
    Authors: Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim YY, Lindeman GJ, Shannon MF, Drew PA, Khew-Goodall Y, Goodall GJ
    Mol. Biol. Cell, 2011;22(10):1686-98.
    Species: Canine
    Sample Types: Cell Culture Supernates
  36. Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
    Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
    J. Bone Miner. Res., 2011;26(8):1891-903.
    Species: Human
    Sample Types: Serum
  37. Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring.
    Authors: Holmlund U, Amoudruz P, Johansson MA, Haileselassie Y, Ongoiba A, Kayentao K, Traore B, Doumbo S, Schollin J, Doumbo O, Montgomery SM, Sverremark-Ekstrom E
    Clin. Exp. Immunol., 2010;162(3):500-9.
    Species: Human
    Sample Types: Milk
  38. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
    Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
    Transplantation, 2009;88(9):1109-16.
    Species: Human
    Sample Types: Urine
  39. Connexin43 knockdown accelerates wound healing but inhibits mesenchymal transition after corneal endothelial injury in vivo.
    Authors: Nakano Y, Oyamada M, Dai P, Nakagami T, Kinoshita S, Takamatsu T
    Invest. Ophthalmol. Vis. Sci., 2008;49(1):93-104.
    Species: Rat
    Sample Types: Aqueous Humor
  40. Connective tissue growth factor as a mediator of intraocular fibrosis.
    Authors: He S, Chen Y, Khankan R, Barron E, Burton R, Zhu D, Ryan SJ, Oliver N, Hinton DR
    Invest. Ophthalmol. Vis. Sci., 2008;49(9):4078-88.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Potent inhibition of cicatricial contraction in proliferative vitreoretinal diseases by statins.
    Authors: Kawahara S, Hata Y, Kita T, Arita R, Miura M, Nakao S, Mochizuki Y, Enaida H, Kagimoto T, Goto Y, Hafezi-Moghadam A, Ishibashi T
    Diabetes, 2008;57(10):2784-93.
    Species: Human
    Sample Types: Vitreous Humor
  42. Interferon-gamma differentially regulates TGF-beta1 and TGF-beta2 expression in human retinal pigment epithelial cells through JAK-STAT pathway.
    Authors: Nagineni CN, Cherukuri KS, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2007;210(1):192-200.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
    Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
    AIDS Res. Hum. Retroviruses, 2007;23(7):886-90.
    Species: Human
    Sample Types: Plasma
  44. Tumor-derived TGFbeta-1 induces dendritic cell apoptosis in the sentinel lymph node.
    Authors: Ito M, Minamiya Y, Kawai H, Saito S, Saito H, Nakagawa T, Imai K, Hirokawa M, Ogawa J
    J. Immunol., 2006;176(9):5637-43.
    Species: Human
    Sample Types: Tissue Homogenates
  45. Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
    Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
    Transpl. Int., 2005;18(2):177-85.
    Species: Human
    Sample Types: Plasma
  46. Effect of ozone exposure on airway responses to inhaled allergen in asthmatic subjects.
    Authors: Chen LL, Tager IB, Peden DB, Christian DL, Ferrando RE, Welch BS, Balmes JR
    Chest, 2004;125(6):2328-35.
    Species: Human
    Sample Types: BALF
  47. Expression of connective tissue growth factor after glaucoma filtration surgery in a rabbit model.
    Authors: Esson DW, Neelakantan A, Iyer SA, Blalock TD, Balasubramanian L, Grotendorst GR, Schultz GS, Sherwood MB
    Invest. Ophthalmol. Vis. Sci., 2004;45(2):485-91.
    Species: Rabbit
    Sample Types: Tissue Homogenates
  48. T cell-specific ablation of Fas leads to Fas ligand-mediated lymphocyte depletion and inflammatory pulmonary fibrosis.
    Authors: Hao Z, Hampel B, Yagita H, Rajewsky K
    J. Exp. Med., 2004;199(10):1355-65.
    Species: Mouse
    Sample Types: Serum
  49. Role of transforming growth factor-beta in breast milk for initiation of IgA production in newborn infants.
    Authors: Ogawa J, Sasahara A, Yoshida T, Sira MM, Futatani T, Kanegane H, Miyawaki T
    Early Hum. Dev., 2004;77(1):67-75.
    Species: Human
    Sample Types: Colostrum
  50. AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
    Authors: Truckenmiller ME, Vawter MP, Zhang P, Conejero-Goldberg C, Dillon-Carter O, Morales N, Cheadle C, Becker KG, Freed WJ
    Exp. Neurol., 2002;175(0):318.
    Species: Rat
    Sample Types: Cell Culture Supernates
  51. Gene transfer of soluble TGF-beta type II receptor inhibits experimental proliferative vitreoretinopathy.
    Authors: Oshima Y, Sakamoto T, Hisatomi T, Tsutsumi C, Ueno H, Ishibashi T
    Gene Ther., 2002;9(18):1214-20.
    Species: Rabbit
    Sample Types: Vitreous Humor
  52. Progesterone upregulates TGF-b isoforms (b1, b2, and b3) expression in normal human osteoblast-like cells.
    Authors: Luo XH, Liao EY, Su X
    Calcif. Tissue Int., 2002;71(4):329-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Circadian variations of transforming growth factor-beta2 and basic fibroblast growth factor in the rabbit aqueous humor.
    Authors: Liu JH
    Curr. Eye Res., 2002;24(1):75-80.
    Species: Rabbit
    Sample Types: Aqueous Humor
  54. Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle.
    Authors: Bracamonte MP, Rud KS, Owen WG, Miller VM
    Am. J. Physiol. Heart Circ. Physiol., 2002;283(3):H853-60.
    Species: Porcine
    Sample Types: Cell Lysates
  55. Transforming growth factor-beta -Smad signaling pathway cooperates with NF-kappa B to mediate nontypeable Haemophilus influenzae-induced MUC2 mucin transcription.
    Authors: Jono H, Shuto T, Xu H, Kai H, Lim DJ, Gum JR, Kim YS, Yamaoka S, Feng XH, Li JD
    J. Biol. Chem., 2002;277(47):45547-57.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Transforming growth factor-beta in breast milk: a potential regulator of atopic disease at an early age.
    Authors: Kalliomaki M, Ouwehand A, Arvilommi H, Kero P, Isolauri E
    J. Allergy Clin. Immunol., 1999;104(6):1251-7.
    Species: Human
    Sample Types: Milk

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Human TGF-beta 2 Quantikine ELISA Kit
By Adam Guess on 02/04/2017
Sample Tested: Cell Culture Supernates