Human TGF-beta  RI/ALK-5 Biotinylated Antibody

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Product Details
Citations (1)
Supplemental Products
Reviews (1)

Human TGF-beta  RI/ALK-5 Biotinylated Antibody Summary

Species Reactivity
Detects human TGF-beta RI/ALK-5 in Western blots.  In Western blots, approximately 50% cross-reactivity with recombinant mouse TGF-beta RI and less than 5% cross-reactivity with recombinant human (rh) TGF-beta RII and rhTGF-beta RIII is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human TGF-beta RI/ALK-5
Accession # P36897
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human TGF-beta RI/ALK-5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RI/ALK-5

Human TGF-beta RI (also ALK5) is a 55 kDa member of the Ser/Thr kinase family of transmembrane proteins. It is a type I membrane glycoprotein that is 479 amino acids (aa) in length. The short 101 aa extracellular domain (ECD) contains multiple cysteines and one potential N-linked glycosylation site. It is ubiquitously expressed, and forms a functional heterotetrameric TGF-beta receptor with TGF-beta RII. TGF-beta binding to TGF-beta RII phosphorylates TGF-beta RI, which then activates SMAD transcriptional proteins. The human TGF-beta RI ECD is 91%, 90%, 91%, and 93% aa identical to the TGF-beta RI ECD in pig, mouse, cow and rat, respectively.

Long Name
Transforming Growth Factor beta Receptor I
Entrez Gene IDs
7046 (Human); 21812 (Mouse); 29591 (Rat)
Alternate Names
AAT5; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; Activin receptor-like kinase 5; ACVRLK4; ALK-5; ALK-5ALK5; EC 2.7.11; EC; LDS1A; LDS2A; Serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta RI; TGF-beta type I receptor; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; transforming growth factor beta receptor I; transforming growth factor, beta receptor 1; transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD); Transforming growth factor-beta receptor type I

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Citation for Human TGF-beta  RI/ALK-5 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-? receptor type I ALK5
    Authors: LK Bollum, K Huse, MP Oksvold, B Bai, VI Hilden, L Forfang, SO Yoon, S Wälchli, EB Smeland, JH Myklebust
    PLoS ONE, 2017;12(5):e0177188.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Human TGF-beta  RI/ALK-5 Biotinylated Antibody
By Anonymous on 09/17/2021
Application: ELISA Sample Tested: Recombinant protein Species: Human

Immobilized TBRII onto ELISA plate. Then incubated plate with TGFb1 + TBRI solution. Detect binding of TBRI to TGFb1+TBRII complex by using Anti-TBRI-biotin. No binding of TBRI only to plate.