Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all TGF-beta RI/ALK-5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TGF-beta RI/ALK-5
Leu34-Glu125
Accession # P36897

Specificity

Detects human TGF-beta RI/ALK-5 in Western blots.  In Western blots, approximately 50% cross-reactivity with recombinant mouse TGF-beta RI and less than 5% cross-reactivity with recombinant human (rh) TGF-beta RII and rhTGF-beta RIII is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human TGF‑ beta  RI/ALK‑5 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TGF-beta RI/ALK-5

Reviewed Applications

Read 1 review rated 4 using BAF3025 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta RI/ALK-5

Human TGF-beta RI (also ALK5) is a 55 kDa member of the Ser/Thr kinase family of transmembrane proteins. It is a type I membrane glycoprotein that is 479 amino acids (aa) in length. The short 101 aa extracellular domain (ECD) contains multiple cysteines and one potential N-linked glycosylation site. It is ubiquitously expressed, and forms a functional heterotetrameric TGF-beta receptor with TGF-beta RII. TGF-beta binding to TGF-beta RII phosphorylates TGF-beta RI, which then activates SMAD transcriptional proteins. The human TGF-beta RI ECD is 91%, 90%, 91%, and 93% aa identical to the TGF-beta RI ECD in pig, mouse, cow and rat, respectively.

Long Name

Transforming Growth Factor beta Receptor I

Alternate Names

ALK-5, SKR4, TGF-bRI, TGFbetaRI, TGFBR1

Entrez Gene IDs

7046 (Human); 21812 (Mouse); 29591 (Rat)

Gene Symbol

TGFBR1

UniProt

P36897

Additional TGF-beta RI/ALK-5 Products

Product Documents for Human TGF‑ beta  RI/ALK‑5 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TGF‑ beta  RI/ALK‑5 Biotinylated Antibody

For research use only

Citations for Human TGF‑ beta  RI/ALK‑5 Biotinylated Antibody

Customer Reviews for Human TGF‑ beta  RI/ALK‑5 Biotinylated Antibody (1)

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  • Human TGF-beta  RI/ALK-5 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 09/17/2021
    Immobilized TBRII onto ELISA plate. Then incubated plate with TGFb1 + TBRI solution. Detect binding of TBRI to TGFb1+TBRII complex by using Anti-TBRI-biotin. No binding of TBRI only to plate.
    Human TGF‑ beta  RI/ALK‑5 Biotinylated Antibody BAF3025

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