Human TGF-beta  RI/ALK-5 Antibody

Catalog # Availability Size / Price Qty
Detection of Human TGF‑ beta  RI/ALK‑5 by Western Blot.
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Product Details
Citations (8)
Supplemental Products

Human TGF-beta  RI/ALK-5 Antibody Summary

Species Reactivity
Detects human TGF-beta RI/ALK-5 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse TGF‑ beta  RI is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human TGF-beta RI/ALK-5
Accession # P36897
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1-0.3 µg/mL
See below

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human TGF‑ beta  RI/ALK‑5 Monoclonal Antibody (Catalog # MAB3025).

This product is intended for assay development on various assay platforms requiring antibody pairs.

5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TGF‑ beta  RI/ALK‑5 antibody by Western Blot. View Larger

Detection of Human TGF‑ beta RI/ALK‑5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TGF-beta RI/ALK-5 at approximately 50 & 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry TGF-beta  RI/ALK-5 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

TGF-beta RI/ALK‑5 in Human PBMCs. TGF-beta RI/ALK-5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RI/ALK-5

TGF-beta RI, also known as activin-like kinase 5 (ALK-5), is a 55 kDa type I transmembrane serine/threonine receptor kinase. It is ubiquitously expressed and forms a functional heterotetrameric signaling TGF-beta receptor complex with TGF-beta RII. The amino acid sequence of human TGF-beta RI extracellular domain is 91%, 90%, 91%, and 93% identical to that of porcine, mouse, bovine, and rat TGF-beta RI, respectively.

Long Name
Transforming Growth Factor beta Receptor I
Entrez Gene IDs
7046 (Human); 21812 (Mouse); 29591 (Rat)
Alternate Names
AAT5; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; Activin receptor-like kinase 5; ACVRLK4; ALK-5; ALK-5ALK5; EC 2.7.11; EC; LDS1A; LDS2A; Serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta RI; TGF-beta type I receptor; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; transforming growth factor beta receptor I; transforming growth factor, beta receptor 1; transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD); Transforming growth factor-beta receptor type I

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Citations for Human TGF-beta  RI/ALK-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma
    Authors: W Cheng, HL Li, SY Xi, XF Zhang, Y Zhu, Le Xing, YX Mo, MM Li, FE Kong, WJ Zhu, XG Chen, HQ Cui, ZM Cao, YF Gong, YQ Tang, Y Zhang, XY Guan, NF Ma, M Liu
    Nature Communications, 2021;12(1):7142.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. TGF&beta1 Suppressed Matrix Mineralization of Osteoblasts Differentiation by Regulating SMURF1-C/EBP&beta-DKK1 Axis
    Authors: B Nam, H Park, YL Lee, Y Oh, J Park, SY Kim, S Weon, SH Choi, JH Yang, S Jo, TH Kim
    International Journal of Molecular Sciences, 2020;21(24):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  3. POH1 contributes to hyperactivation of TGF-? signaling and facilitates hepatocellular carcinoma metastasis through deubiquitinating TGF-? receptors and caveolin-1
    Authors: B Wang, X Xu, Z Yang, L Zhang, Y Liu, A Ma, G Xu, M Tang, T Jing, L Wu, Y Liu
    EBioMedicine, 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. MDM2 promotes epithelial-mesenchymal transition and metastasis of ovarian cancer SKOV3 cells.
    Authors: Ying Chen, Dan-Dan Wang, Ye-Ping Wu, Dan Su, Tian-Yi Zhou, Ren-Hua Gai, Ying-Ying Fu, Lin Zheng, Qiao-Jun He, Hong Zhu, Bo Yang
    British Journal of Cancer, 2017;0(0):1532-1827.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. MiR-142-3p represses TGF-beta-induced growth inhibition through repression of TGFbetaR1 in non-small cell lung cancer.
    Authors: Lei Z, Xu G, Wang L, Yang H, Liu X, Zhao J, Zhang H
    FASEB J, 2014;28(6):2696-704.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Key role of the endothelial TGF-beta/ALK1/endoglin signaling pathway in humans and rodents pulmonary hypertension.
    Authors: Gore B, Izikki M, Mercier O, Dewachter L, Fadel E, Humbert M, Dartevelle P, Simonneau G, Naeije R, Lebrin F, Eddahibi S
    PLoS ONE, 2014;9(6):e100310.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Activin-like kinase receptor 1 (ALK1) in atherosclerotic lesions and vascular mesenchymal cells.
    Authors: Yao Y, Zebboudj AF, Torres A, Shao E, Bostrom K
    Cardiovasc. Res., 2007;74(2):279-89.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Analysis of the interacting partners of the neuronal calcium-binding proteins L-CaBP1, hippocalcin, NCS-1 and neurocalcin delta.
    Authors: Haynes LP, Fitzgerald DJ, Wareing B, O'Callaghan DW, O'Callaghan DW, Morgan A, Burgoyne RD
    Proteomics, 2006;6(6):1822-32.
    Species: Bovine
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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