Human TGF-beta RI/ALK-5 Antibody
Human TGF-beta RI/ALK-5 Antibody Summary
Leu34-Glu125
Accession # P36897
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Applications
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human TGF‑ beta RI/ALK‑5 Monoclonal Antibody (Catalog # MAB3025).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human TGF‑ beta RI/ALK‑5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TGF-beta RI/ALK-5 at approximately 50 & 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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TGF-beta RI/ALK‑5 in Human PBMCs. TGF-beta RI/ALK-5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta RI/ALK-5
TGF-beta RI, also known as activin-like kinase 5 (ALK-5), is a 55 kDa type I transmembrane serine/threonine receptor kinase. It is ubiquitously expressed and forms a functional heterotetrameric signaling TGF-beta receptor complex with TGF-beta RII. The amino acid sequence of human TGF-beta RI extracellular domain is 91%, 90%, 91%, and 93% identical to that of porcine, mouse, bovine, and rat TGF-beta RI, respectively.
Product Datasheets
Citations for Human TGF-beta RI/ALK-5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma
Authors: W Cheng, HL Li, SY Xi, XF Zhang, Y Zhu, Le Xing, YX Mo, MM Li, FE Kong, WJ Zhu, XG Chen, HQ Cui, ZM Cao, YF Gong, YQ Tang, Y Zhang, XY Guan, NF Ma, M Liu
Nature Communications, 2021;12(1):7142.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
TGF&beta1 Suppressed Matrix Mineralization of Osteoblasts Differentiation by Regulating SMURF1-C/EBP&beta-DKK1 Axis
Authors: B Nam, H Park, YL Lee, Y Oh, J Park, SY Kim, S Weon, SH Choi, JH Yang, S Jo, TH Kim
International Journal of Molecular Sciences, 2020;21(24):.
Species: Human
Sample Types: Protein
Applications: Western Blot -
POH1 contributes to hyperactivation of TGF-? signaling and facilitates hepatocellular carcinoma metastasis through deubiquitinating TGF-? receptors and caveolin-1
Authors: B Wang, X Xu, Z Yang, L Zhang, Y Liu, A Ma, G Xu, M Tang, T Jing, L Wu, Y Liu
EBioMedicine, 2019;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
MDM2 promotes epithelial-mesenchymal transition and metastasis of ovarian cancer SKOV3 cells.
Authors: Ying Chen, Dan-Dan Wang, Ye-Ping Wu, Dan Su, Tian-Yi Zhou, Ren-Hua Gai, Ying-Ying Fu, Lin Zheng, Qiao-Jun He, Hong Zhu, Bo Yang
British Journal of Cancer, 2017;0(0):1532-1827.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
MiR-142-3p represses TGF-beta-induced growth inhibition through repression of TGFbetaR1 in non-small cell lung cancer.
Authors: Lei Z, Xu G, Wang L, Yang H, Liu X, Zhao J, Zhang H
FASEB J, 2014;28(6):2696-704.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Key role of the endothelial TGF-beta/ALK1/endoglin signaling pathway in humans and rodents pulmonary hypertension.
Authors: Gore B, Izikki M, Mercier O, Dewachter L, Fadel E, Humbert M, Dartevelle P, Simonneau G, Naeije R, Lebrin F, Eddahibi S
PLoS ONE, 2014;9(6):e100310.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Activin-like kinase receptor 1 (ALK1) in atherosclerotic lesions and vascular mesenchymal cells.
Authors: Yao Y, Zebboudj AF, Torres A, Shao E, Bostrom K
Cardiovasc. Res., 2007;74(2):279-89.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Analysis of the interacting partners of the neuronal calcium-binding proteins L-CaBP1, hippocalcin, NCS-1 and neurocalcin delta.
Authors: Haynes LP, Fitzgerald DJ, Wareing B, O'Callaghan DW, O'Callaghan DW, Morgan A, Burgoyne RD
Proteomics, 2006;6(6):1822-32.
Species: Bovine
Sample Types: Tissue Homogenates
Applications: Western Blot
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