Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Bovine

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TGF-beta RI/ALK-5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TGF-beta RI/ALK-5
Leu34-Glu125
Accession # P36897

Specificity

Detects human TGF-beta RI/ALK-5 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse TGF‑ beta  RI is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TGF‑ beta  RI/ALK‑5 Antibody

Detection of Human TGF‑ beta RI/ALK‑5 antibody by Western Blot.

Detection of Human TGF‑ beta RI/ALK‑5 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TGF-beta RI/ALK-5 at approximately 50 & 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TGF-beta RI/ALK-5 antibody in Human PBMCs by Immunocytochemistry (ICC).

TGF-beta RI/ALK‑5 in Human PBMCs.

TGF-beta RI/ALK-5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Porcine TGF-beta RI/ALK-5 by Western Blot

Detection of Porcine TGF-beta RI/ALK-5 by Western Blot

Myostatin signaling in longissimus dorsi muscle of low-birth-weight (LBWT) and normal-birth-weight (NBWT) neonatal pigs. (A): Representative western blots from four pairs of LBWT and NBWT pigs (N, NBWT; L, LBWT). (B–G): Protein abundance of myostain, ALK5 and ActRIIB, and protein abundance phosphorylation of smad2/3. Abundance was normalized to alpha -tubulin and phosphorylation normalized to the corresponding non-phospho-proteins. Results are means ± SE. n = 12. Values with different letters differ significantly (P ≤ 0.05). Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fphys.2017.00482/full), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TGF-beta RI/ALK-5 by Western Blot

Detection of TGF-beta RI/ALK-5 by Western Blot

WT1 regulates the STAT1/3 pathway in CAFs. A Protein levels of WT1, STAT1, STAT3, PD-L1 and the TGF beta receptor were determined in WT1 knockdown (A) and WT1 overexpressed (B) CAFs by Western blotting. Actin was used as a loading control. IDO release was determined by ELISA. RNA levels of PD-L1 was determined by qRT-PCR. TBP was used as a loading control. Representative experiment is shown of n = 3–5 biological replicates. Data represents mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40598415), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TGF‑ beta  RI/ALK‑5 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human TGF‑ beta  RI/ALK‑5 Monoclonal Antibody (Catalog # MAB3025).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells

Western Blot

0.1-0.3 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitt's lymphoma cell line

Reviewed Applications

Read 2 reviews rated 5 using AF3025 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta RI/ALK-5

TGF-beta RI, also known as activin-like kinase 5 (ALK-5), is a 55 kDa type I transmembrane serine/threonine receptor kinase. It is ubiquitously expressed and forms a functional heterotetrameric signaling TGF-beta receptor complex with TGF-beta RII. The amino acid sequence of human TGF-beta RI extracellular domain is 91%, 90%, 91%, and 93% identical to that of porcine, mouse, bovine, and rat TGF-beta RI, respectively.

Long Name

Transforming Growth Factor beta Receptor I

Alternate Names

ALK-5, SKR4, TGF-bRI, TGFbetaRI, TGFBR1

Entrez Gene IDs

7046 (Human); 21812 (Mouse); 29591 (Rat)

Gene Symbol

TGFBR1

UniProt

P36897

Additional TGF-beta RI/ALK-5 Products

Product Documents for Human TGF‑ beta  RI/ALK‑5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TGF‑ beta  RI/ALK‑5 Antibody

For research use only

Citations for Human TGF‑ beta  RI/ALK‑5 Antibody

Customer Reviews for Human TGF‑ beta  RI/ALK‑5 Antibody (2)

5 out of 5
2 Customer Ratings
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Customer Images

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  • Human TGF-beta  RI/ALK-5 Antibody
    Name: Jenna Nguyen
    Application: Affinity Purification
    Sample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell line
    Species: Mouse
    Verified Customer | Posted 05/07/2019
  • Human TGF-beta  RI/ALK-5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver cells
    Species: Human
    Verified Customer | Posted 08/20/2018
    Human TGF‑ beta  RI/ALK‑5 Antibody AF3025

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