Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than 2% cross‑reactivity with recombinant human (rh) TGF‑ beta 3 and recombinant amphibian TGF‑ beta 5 and no cross-reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.
Monoclonal Mouse IgG1 Clone # 9016
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant TGF‑ beta 1 and latent TGF‑ beta 1
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are
available in the Technical Information section on our website.
|Intracellular Staining by Flow Cytometry
Detection of TGF-beta 1 in PC-3 human prostate cancer cell line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TGF-beta 1 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC240F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Background: TGF-beta 1
TGF-beta 1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.
Transforming Growth Factor beta 1
Entrez Gene IDs:
7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine);
TGFB1; CEDLAP; DPD1; latency-associated peptide; TGFB; TGFbeta; TGF-beta 1 protein; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1
Related Research Areas
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