Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than 2% cross‑reactivity with recombinant human (rh) TGF‑ beta 3 and recombinant amphibian TGF‑ beta 5 and no cross-reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.
Monoclonal Mouse IgG1 Clone # 9016
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant TGF‑ beta 1 and latent TGF‑ beta 1
Supplied in a saline solution containing BSA and Sodium Azide.
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of TGF‑ beta 1 in PC‑3 human prostate cancer cell line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human TGF‑ beta 1 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC240F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: TGF-beta 1
TGF-beta 1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.