Human TGF-beta 1 Fluorescein-conjugated Antibody

Name: Human TGF-beta 1 Fluorescein-conjugated Antibody
Brand: R&D Systems
SKU: IC240F

Catalog #: IC240F
1 Citation Datasheet / COA / SDS

Discontinued Product

IC240F has been discontinued.

View all TGF-beta 1 products.

Detection of TGF‑ beta 1 in PC‑3 human prostate cancer cell line by Flow Cytometry.

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Citations (1)

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Human TGF-beta 1 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than 2% cross‑reactivity with recombinant human (rh) TGF‑ beta 3 and recombinant amphibian TGF‑ beta 5 and no cross-reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 9016

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant TGF‑ beta 1 and latent TGF‑ beta 1

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of TGF-beta 1 antibody in PC-3 human prostate cancer cell line antibody by Flow Cytometry.

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Detection of TGF‑ beta 1 in PC‑3 human prostate cancer cell line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TGF-beta 1 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC240F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta 1

TGF-beta 1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

Long Name

Transforming Growth Factor beta 1

Entrez Gene IDs

7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)

Alternate Names

CEDLAP; DPD1; latency-associated peptide; TGF beta; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human TGF-beta 1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells.
Authors: Ansa-Addo EA, Lange S, Stratton D, Antwi-Baffour S, Cestari I, Ramirez MI, McCrossan MV, Inal JM
J. Immunol., 2010-10-04;185(9):5236-46.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry