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OR

Human TGF‑ beta 1 PE‑conjugated Antibody

4 citations
  • Specificity
    Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than 2% cross‑reactivity with recombinant human (rh) TGF‑ beta 3 and recombinant amphibian TGF‑ beta 5 and no cross‑reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 9016
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant TGF‑ beta 1 and latent TGF‑ beta 1
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of TGF-beta 1 in PC-3 human prostate cancer cell line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TGF-beta 1 PE-conjugated Monoclonal Antibody (Catalog # IC240P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Transforming Growth Factor beta 1
TGF-beta 1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.
  • Long Name:
    Transforming Growth Factor beta 1
  • Entrez Gene IDs:
    7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine);
  • Alternate Names:
    CEDLAP; DPD1; latency-associated peptide; TGFB; TGFbeta; TGF-beta 1 protein; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 4 of 4
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Species
Applications
  1. Development of a nascent galectin-1 chimeric molecule for studying the role of leukocyte galectin-1 ligands and immune disease modulation.
    Authors: Cedeno-Laurent F, Barthel SR, Opperman MJ, Lee DM, Clark RA, Dimitroff CJ
    J. Immunol., 2010;185(8):4659-72.
    Species: Human
    Application: Flow Cytometry,
  2. Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
    Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
    J. Immunol., 2008;180(7):5109-17.
    Species: Human
    Application: Flow Cytometry,
  3. Expression of the T cell receptor alpha/beta on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
    Authors: Thiel A, Kesselring R, Pries R, Puzik A, Wittkopf N, Wollenberg B
    PLoS ONE, 2011;6(1):e15997.
    Species: Human
    Application: Flow Cytometry,
  4. Identification of IDO-positive and IDO-negative human dendritic cells after activation by various proinflammatory stimuli.
    Authors: Von Bubnoff D, Scheler M, Wilms H, Fimmers R, Bieber T
    J. Immunol., 2011;186(12):6701-9.

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  • Catalog #
    IC240P

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