Human Thimet Oligopeptidase/THOP1 Antibody

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Product Details
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Human Thimet Oligopeptidase/THOP1 Antibody Summary

Species Reactivity
Detects human Thimet Oligopeptidase/THOP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human Neurolysin is observed.
Monoclonal Mouse IgG1 Clone # 455710
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Thimet Oligopeptidase/THOP1
Accession # P52888
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Thimet Oligopeptidase/THOP1 (Catalog # 3439-ZN)
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Thimet Oligopeptidase/THOP1 (Catalog # 3439-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thimet Oligopeptidase/THOP1

Thimet Oligopeptidase/THOP1, also known as endopeptidase EC (EP24.15), is a zinc peptidase of the M3 family that also includes neurolysin/EC and mitochondrial intermediate peptidase (1). Widely expressed by mammalian tissues and reported to present in different subcellular locations, THOP1 is primarily a cytoplasmic enzyme. It is capable of hydrolyzing a number of bioactive peptides and peptides released by the proteosome, limiting antigenic presentation by MHC class I molecules (1‑3). THOP1 also interacts with angiotensin II type I receptor and bradykinin B2 receptor (4). The amino acid sequence of human THOP1 is 90% and 86% identical to that of dog/mouse and rat, respectively.

  1. Barrett, A.J. and J.-M. Chen (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 352, Elsevier Academic Press, San Diego.
  2. Ray, K. et al. (2004) J. Biol. Chem. 279:20480.
  3. Saric, T. et al. (2004) J. Biol. Chem. 279:46723.
  4. Shivakumar, B.R. et al. (2005) Cell Biochem. Funct. 23:195.
Entrez Gene IDs
7064 (Human); 50492 (Mouse); 64517 (Rat)
Alternate Names
EC 3.4.24; EC; Endopeptidase 24.15; EP24.15; MEPD_HUMAN; MP78; thimet oligopeptidase 1; thimet oligopeptidase; THOP1; TOP

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