Human Thrombospondin-1 Biotinylated Antibody

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Product Details
Citations (1)
Supplemental Products

Human Thrombospondin-1 Biotinylated Antibody Summary

Species Reactivity
Detects Thrombospondin-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) Thrombospondin-2 and rhThrombospondin-4 is observed.  
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-1
Accession # P07996
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Thrombospondin-1 (Catalog # 3074-TH)

Human Thrombospondin-1 Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Thrombospondin‑1 Antibody (Catalog # MAB3074)

Standard: Recombinant Human Thrombospondin-1 Protein, CF (Catalog # 3074-TH)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombospondin-1

Thrombospondin-1 (TSP-1) is a 150‑180 kDa charter member of the thrombospondin family of extracellular matrix proteins. Human TSP‑1 is synthesized as an 1170 amino acid (aa) precursor that contains an 18 aa signal sequence and 1152 aa mature molecule. The mature molecule has been described as containing three distinct regions that create the shape of a dumbbell. There is an intital, 140 aa N-terminal laminin G-like globular region that binds heparin (aa 19‑258). This is followed by an extended, central collagen-binding region that contains one type C von Willebrand factor domain, plus three TSP type I and three TSP type II (or EGF-like) domains (aa 259‑712). The C‑terminus (aa 713‑1170) appears as a large globule with two halves; one calcium‑binding region (aa 713‑950) with seven Asp‑rich TSP type III domains, and one terminal region (aa 951‑1170) with TSP‑unique motifs (1). This C-terminal region is believed to mediate CD47 and cell binding (2‑5). The TSP type I repeats have multiple functions. They bind to type V collagen, laminin, fibronectin and CD36. They also contain a recognition site for C‑mannosylation on Trp. Finally, a type I KRFK motif induces the release of mature TGF-beta from LAP. This is an effect not found in TSP-2. The function of the type II repeats in unclear. TSP‑1 is secreted as a disulfide-linked 450 kDa homotrimer. The cysteines responsible lie just N‑terminal to the first type I TSP repeat. Mature human TSP-1 is 61% aa identical to human TSP-2. It is also 95%, 97% and 95% aa identical to mouse, dog and rat TSP-1, respectively.

  1. Lawler, J. and R.O. Hynes (1986) J. Cell Biol. 103:1635.
  2. Frazier, W.A. (1987) J. Cell Biol. 105:625.
  3. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
  4. Sid, B. et al. (2004) Crit. Rev. Oncol. Hematol. 49:245.
  5. Floquet, N. et al. (2008) Anch. Biochem. Biophys. 478:103.
Entrez Gene IDs
7057 (Human); 21825 (Mouse)
Alternate Names
THBS; THBS1; THBS-1; thrombospondin 1; Thrombospondin1; Thrombospondin-1; thrombospondin-1p180; TSP-1; TSP1thrombospondin-1; TSPTSP-1

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Citation for Human Thrombospondin-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Development and clinical testing of individual immunoassays for the quantification of serum glycoproteins to diagnose prostate cancer
    Authors: K Endt, J Goepfert, A Omlin, A Athanasiou, P Tennstedt, A Guenther, M Rainisio, DS Engeler, T Steuber, S Gillessen, T Joos, R Schiess
    PLoS ONE, 2017;12(8):e0181557.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Detection)


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