Human Thrombospondin-1 Antibody

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(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Thrombospondin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Thrombospondin-2 and rhThrombospondin-4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-1
    Asn19-Pro1170
    Accession # CAA32889
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Thrombospondin‑1 by Western Blot. Western blot shows lysates of MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Thrombospondin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Thrombospondin‑1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Thrombospondin‑1 in Human Colon. Thrombospondin‑1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human Thrombospondin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Thrombospondin‑1 by Simple WesternTM. Simple Western lane view shows lysates of MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Thrombospondin‑1 at approximately 221 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Thrombospondin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thrombospondin-1

Thrombospondin-1 (TSP-1) is a 150‑180 kDa charter member of the thrombospondin family of extracellular matrix proteins. Human TSP‑1 is synthesized as an 1170 amino acid (aa) precursor that contains an 18 aa signal sequence and 1152 aa mature molecule. The mature molecule has been described as containing three distinct regions that create the shape of a dumbbell. There is an intital, 140 aa N-terminal laminin G-like globular region that binds heparin (aa 19‑258). This is followed by an extended, central collagen-binding region that contains one type C von Willebrand factor domain, plus three TSP type I and three TSP type II (or EGF-like) domains (aa 259‑712). The C‑terminus (aa 713‑1170) appears as a large globule with two halves; one calcium‑binding region (aa 713‑950) with seven Asp‑rich TSP type III domains, and one terminal region (aa 951‑1170) with TSP‑unique motifs (1). This C-terminal region is believed to mediate CD47 and cell binding (2‑5). The TSP type I repeats have multiple functions. They bind to type V collagen, laminin, fibronectin and CD36. They also contain a recognition site for C‑mannosylation on Trp. Finally, a type I KRFK motif induces the release of mature TGF-b from LAP. This is an effect not found in TSP-2. The function of the type II repeats in unclear. TSP‑1 is secreted as a disulfide-linked 450 kDa homotrimer. The cysteines responsible lie just N‑terminal to the first type I TSP repeat. Mature human TSP-1 is 61% aa identical to human TSP-2. It is also 95%, 97% and 95% aa identical to mouse, dog and rat TSP-1, respectively.

  • References:
    1. Lawler, J. and R.O. Hynes (1986) J. Cell Biol. 103:1635.
    2. Frazier, W.A. (1987) J. Cell Biol. 105:625.
    3. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
    4. Sid, B. et al. (2004) Crit. Rev. Oncol. Hematol. 49:245.
    5. Floquet, N. et al. (2008) Anch. Biochem. Biophys. 478:103.
  • Entrez Gene IDs:
    7057 (Human); 21825 (Mouse)
  • Alternate Names:
    THBS; THBS1; THBS-1; thrombospondin 1; Thrombospondin1; Thrombospondin-1; thrombospondin-1p180; TSP-1; TSP1thrombospondin-1; TSPTSP-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Canonical wnt signaling regulates extracellular matrix expression in the trabecular meshwork.
    Authors: Villarreal G, Chatterjee A, Oh S, Oh D, Kang M, Rhee D
    Invest Ophthalmol Vis Sci, 2015;55(11):7433-40.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human Thrombospondin-1 Antibody AF3074
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Very Good
 WB Human Anonymous 05/20/2016
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Western Blot Human Thrombospondin-1 Antibody AF3074
Western Blot: Human Thrombospondin-1 Antibody [AF3074]

Summary

ApplicationWestern Blot
Sample TestedMesenchymal stem cells
SpeciesHuman

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