Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Dot Blot, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Thrombospondin-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-1
Asn19-Pro1170
Accession # CAA32889

Specificity

Detects human Thrombospondin-1 in direct ELISAs.  In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh)Thrombospondin-2 and rhThrombospondin-4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Thrombospondin‑1 Antibody

Detection of Human Thrombospondin‑1 antibody by Western Blot.

Detection of Human Thrombospondin‑1 by Western Blot.

Western blot shows lysates of MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Thrombospondin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Thrombospondin-1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Thrombospondin‑1 by Simple WesternTM.

Simple Western lane view shows lysates of Exosome Standards (PC‑3) (NBP2-49856) and MDA‑MB‑231 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for Thrombospondin‑1 at approximately 210 kDa (as indicated) using 10 µg/ml of Goat Anti-Human Thrombospondin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Thrombospondin-1 antibody in Human Colon by Immunohistochemistry (IHC-P).

Thrombospondin‑1 in Human Colon.

Thrombospondin-1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human Thrombospondin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Thrombospondin-1 antibody by Simple WesternTM.

Detection of Human Thrombospondin‑1 by Simple WesternTM.

Simple Western lane view shows lysates of MBA-MB-468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Thrombospondin-1 at approximately 221 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Thrombospondin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Mouse Thrombospondin-1 by Western Blot

Detection of Mouse Thrombospondin-1 by Western Blot

Endothelial cells and TGF beta are not affected by cardiac Thbs1 overexpression.a Quantification of capillary number per mm2 of tissue from histological sections of tTA cont. and Thbs1 DTG hearts stained with isolectin B4 at 6 weeks of age. b Quantification of endothelial cell proliferation as measured by EdU incorporation co-labeled with CD31 in tTA cont. and Thbs1 DTG hearts at 6 weeks of age. c Quantification of endothelial cell apoptosis detected by TUNEL staining co-labeled with isolectin B4 in tTA cont. and Thbs1 DTG hearts at 6 weeks of age. d ELISA-based quantification of total TGF beta and e active TGF beta in protein extracts from tTA cont. or Thbs1 DTG hearts at 6 weeks of age. f Schematic diagram of WT Thbs1 domain structure and the Thbs1 delta t1 mutant lacking the Thbs1 type-1 repeat domain region. g Representative western blot analysis for Thbs1 from total protein extracts (Total) and extracellular matrix (ECM) extracts from hearts of tTA cont., Thbs1 DTG, and Thbs1 DTG delta t1 mice at 4 weeks of age. Vinculin is presented as cytosolic control. Coomassie stained (Coom.) gel is shown as loading control. h VW/BW ratio at 4 weeks of age in the indicated groups of mice. *P < 0.0001 versus tTA cont.; statistical analysis was performed using one-way ANOVA and Tukey multiple comparisons test. i Kaplan–Meier survival plot of tTA cont., Thbs1 DTG, and Thbs1 delta t1 DTG animals. *P < 0.0001 vs tTA cont. #P < 0.0001 vs Thbs1 DTG; both analyzed by two-tailed log-rank test. The same data from Fig. 2e are shown again here for tTA cont. and Thbs1 DTG mice (same strain and ages and sex ratio mix). j Representative western blots for Thbs2 and Gapdh as loading control, from heart protein extracts from tTA cont. and Thbs2 DTG mice at 8 weeks of age. k Heart weight (HW)/BW ratio, and l FS percentage at 8 weeks of age from tTA cont. and Thbs2 DTG mice. The number of biologically independent animals analyzed is indicated on each graph. Error bars are ±standard error of the mean. Source data are provided as a Source Data File. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34168130), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Thrombospondin‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Simple Western

10 µg/mL
Sample: Exosome Standards (PC-3) (Catalog # NBP2-49856), MDA-MB-231 human breast cancer cell line and MDA‑MB‑468 human breast cancer cell line

Western Blot

1 µg/mL
Sample: MDA‑MB‑231 human breast cancer cell line

Reviewed Applications

Read 3 reviews rated 4.7 using AF3074 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombospondin-1

Thrombospondin-1 (TSP-1) is a 150‑180 kDa charter member of the thrombospondin family of extracellular matrix proteins. Human TSP‑1 is synthesized as an 1170 amino acid (aa) precursor that contains an 18 aa signal sequence and 1152 aa mature molecule. The mature molecule has been described as containing three distinct regions that create the shape of a dumbbell. There is an intital, 140 aa N-terminal laminin G-like globular region that binds heparin (aa 19‑258). This is followed by an extended, central collagen-binding region that contains one type C von Willebrand factor domain, plus three TSP type I and three TSP type II (or EGF-like) domains (aa 259‑712). The C‑terminus (aa 713‑1170) appears as a large globule with two halves; one calcium‑binding region (aa 713‑950) with seven Asp‑rich TSP type III domains, and one terminal region (aa 951‑1170) with TSP‑unique motifs (1). This C-terminal region is believed to mediate CD47 and cell binding (2‑5). The TSP type I repeats have multiple functions. They bind to type V collagen, laminin, fibronectin and CD36. They also contain a recognition site for C‑mannosylation on Trp. Finally, a type I KRFK motif induces the release of mature TGF-b from LAP. This is an effect not found in TSP-2. The function of the type II repeats in unclear. TSP‑1 is secreted as a disulfide-linked 450 kDa homotrimer. The cysteines responsible lie just N‑terminal to the first type I TSP repeat. Mature human TSP-1 is 61% aa identical to human TSP-2. It is also 95%, 97% and 95% aa identical to mouse, dog and rat TSP-1, respectively.

References

  1. Lawler, J. and R.O. Hynes (1986) J. Cell Biol. 103:1635.
  2. Frazier, W.A. (1987) J. Cell Biol. 105:625.
  3. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
  4. Sid, B. et al. (2004) Crit. Rev. Oncol. Hematol. 49:245.
  5. Floquet, N. et al. (2008) Anch. Biochem. Biophys. 478:103.

Alternate Names

THBS1, Thrombospondin1, TSP-1

Entrez Gene IDs

7057 (Human); 21825 (Mouse)

Gene Symbol

THBS1

UniProt

CAA32889

Additional Thrombospondin-1 Products

Product Documents for Human Thrombospondin‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Thrombospondin‑1 Antibody

For research use only

Citations for Human Thrombospondin‑1 Antibody

Customer Reviews for Human Thrombospondin‑1 Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
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1 Stars
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Showing  1 - 3 of 3 reviews Showing All
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  • Human Thrombospondin-1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 10/04/2019
    We used this antibody for a sandwich ELISA in combination with mAb (MAB3074)) and protein (3074-TH). This combination works very well for detecting the TSP1 in human serum and plasma samples.
    Human Thrombospondin‑1 Antibody AF3074
  • Human Thrombospondin-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Trabecular meshwork
    Species: Human
    Verified Customer | Posted 01/16/2018
    Human Thrombospondin‑1 Antibody AF3074
  • Human Thrombospondin-1 Antibody
    Name: Juliana Redondo
    Application: Western Blot
    Sample Tested: Mesenchymal stem cells
    Species: Human
    Verified Customer | Posted 05/20/2016
    Human Thrombospondin‑1 Antibody AF3074

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