Human Thrombospondin-1 Antibody

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Product Details
Citations (3)
Supplemental Products
Reviews (1)

Human Thrombospondin-1 Antibody Summary

Species Reactivity
Detects human Thrombospondin‑1 in ELISAs and Western blots. In sandwich immunoassays and Western blots, no cross‑reactivity with recombinant human (rh) Thrombospindin-2 or rhThrombospondin-4 is observed.
Monoclonal Mouse IgG2B Clone # 301221
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-1
Accession # CAA32889
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Thrombospondin‑1 (Catalog # 3074-TH) under non-reducing conditions only
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Thrombospondin‑1 (Catalog # 3074-TH), see our available Western blot detection antibodies

Human Thrombospondin-1 Sandwich Immunoassay

Recommended Concentration
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Thrombospondin‑1 Biotinylated Antibody (Catalog # BAF3074)

Standard: Recombinant Human Thrombospondin-1 Protein, CF (Catalog # 3074-TH)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombospondin-1

Thrombospondin-1 (TSP-1) is a 150‑180 kDa charter member of the thrombospondin family of extracellular matrix proteins. Human TSP‑1 is synthesized as an 1170 amino acid (aa) precursor that contains an 18 aa signal sequence and 1152 aa mature molecule. The mature molecule has been described as containing three distinct regions that create the shape of a dumbbell. There is an intital, 140 aa N-terminal laminin G-like globular region that binds heparin (aa 19‑258). This is followed by an extended, central collagen-binding region that contains one type C von Willebrand factor domain, plus three TSP type I and three TSP type II (or EGF-like) domains (aa 259‑712). The C‑terminus (aa 713‑1170) appears as a large globule with two halves; one calcium‑binding region (aa 713‑950) with seven Asp‑rich TSP type III domains, and one terminal region (aa 951‑1170) with TSP‑unique motifs (1). This C-terminal region is believed to mediate CD47 and cell binding (2‑5). The TSP type I repeats have multiple functions. They bind to type V collagen, laminin, fibronectin and CD36. They also contain a recognition site for C‑mannosylation on Trp. Finally, a type I KRFK motif induces the release of mature TGF-b from LAP. This is an effect not found in TSP-2. The function of the type II repeats in unclear. TSP‑1 is secreted as a disulfide-linked 450 kDa homotrimer. The cysteines responsible lie just N‑terminal to the first type I TSP repeat. Mature human TSP-1 is 61% aa identical to human TSP-2. It is also 95%, 97% and 95% aa identical to mouse, dog and rat TSP-1, respectively.

  1. Lawler, J. and R.O. Hynes (1986) J. Cell Biol. 103:1635.
  2. Frazier, W.A. (1987) J. Cell Biol. 105:625.
  3. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
  4. Sid, B. et al. (2004) Crit. Rev. Oncol. Hematol. 49:245.
  5. Floquet, N. et al. (2008) Anch. Biochem. Biophys. 478:103.
Entrez Gene IDs
7057 (Human); 21825 (Mouse)
Alternate Names
THBS; THBS1; THBS-1; thrombospondin 1; Thrombospondin1; Thrombospondin-1; thrombospondin-1p180; TSP-1; TSP1thrombospondin-1; TSPTSP-1

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Citations for Human Thrombospondin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease
    Authors: WH Kahr, FG Pluthero, A Elkadri, N Warner, M Drobac, CH Chen, RW Lo, L Li, R Li, Q Li, C Thoeni, J Pan, G Leung, I Lara-Corra, R Murchie, E Cutz, RM Laxer, J Upton, CM Roifman, RS Yeung, JH Brumell, AM Muise
    Nat Commun, 2017;8(0):14816.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Development and clinical testing of individual immunoassays for the quantification of serum glycoproteins to diagnose prostate cancer
    Authors: K Endt, J Goepfert, A Omlin, A Athanasiou, P Tennstedt, A Guenther, M Rainisio, DS Engeler, T Steuber, S Gillessen, T Joos, R Schiess
    PLoS ONE, 2017;12(8):e0181557.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  3. Normal ranges of angiogenesis regulatory proteins in human platelets.
    Authors: Peterson JE, Zurakowski D, Italiano JE
    Am. J. Hematol., 2010;85(7):487-93.
    Species: Human
    Sample Types: Cell Lysates


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Human Thrombospondin-1 Antibody
By Anonymous on 10/04/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody for a sandwich ELISA in combination with pAb (AF3074)) and protein (3074-TH). This combination works very well for detecting the TSP1 in human serum and plasma samples.