Human Thrombospondin-1 Antibody
Human Thrombospondin-1 Antibody Summary
Asn19-Pro1170
Accession # CAA32889
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Applications
Human Thrombospondin-1 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thrombospondin-1
Thrombospondin-1 (TSP-1) is a 150‑180 kDa charter member of the thrombospondin family of extracellular matrix proteins. Human TSP‑1 is synthesized as an 1170 amino acid (aa) precursor that contains an 18 aa signal sequence and 1152 aa mature molecule. The mature molecule has been described as containing three distinct regions that create the shape of a dumbbell. There is an intital, 140 aa N-terminal laminin G-like globular region that binds heparin (aa 19‑258). This is followed by an extended, central collagen-binding region that contains one type C von Willebrand factor domain, plus three TSP type I and three TSP type II (or EGF-like) domains (aa 259‑712). The C‑terminus (aa 713‑1170) appears as a large globule with two halves; one calcium‑binding region (aa 713‑950) with seven Asp‑rich TSP type III domains, and one terminal region (aa 951‑1170) with TSP‑unique motifs (1). This C-terminal region is believed to mediate CD47 and cell binding (2‑5). The TSP type I repeats have multiple functions. They bind to type V collagen, laminin, fibronectin and CD36. They also contain a recognition site for C‑mannosylation on Trp. Finally, a type I KRFK motif induces the release of mature TGF-b from LAP. This is an effect not found in TSP-2. The function of the type II repeats in unclear. TSP‑1 is secreted as a disulfide-linked 450 kDa homotrimer. The cysteines responsible lie just N‑terminal to the first type I TSP repeat. Mature human TSP-1 is 61% aa identical to human TSP-2. It is also 95%, 97% and 95% aa identical to mouse, dog and rat TSP-1, respectively.
- Lawler, J. and R.O. Hynes (1986) J. Cell Biol. 103:1635.
- Frazier, W.A. (1987) J. Cell Biol. 105:625.
- Sid, B. et al. (2004) Crit. Rev. Oncol. Hematol. 49:245.
- Floquet, N. et al. (2008) Anch. Biochem. Biophys. 478:103.
Product Datasheets
Citations for Human Thrombospondin-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease
Authors: WH Kahr, FG Pluthero, A Elkadri, N Warner, M Drobac, CH Chen, RW Lo, L Li, R Li, Q Li, C Thoeni, J Pan, G Leung, I Lara-Corra, R Murchie, E Cutz, RM Laxer, J Upton, CM Roifman, RS Yeung, JH Brumell, AM Muise
Nat Commun, 2017;8(0):14816.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Development and clinical testing of individual immunoassays for the quantification of serum glycoproteins to diagnose prostate cancer
Authors: K Endt, J Goepfert, A Omlin, A Athanasiou, P Tennstedt, A Guenther, M Rainisio, DS Engeler, T Steuber, S Gillessen, T Joos, R Schiess
PLoS ONE, 2017;12(8):e0181557.
Species: Human
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture) -
Normal ranges of angiogenesis regulatory proteins in human platelets.
Authors: Peterson JE, Zurakowski D, Italiano JE
Am. J. Hematol., 2010;85(7):487-93.
Species: Human
Sample Types: Cell Lysates
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Reviews for Human Thrombospondin-1 Antibody
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We used this antibody for a sandwich ELISA in combination with pAb (AF3074)) and protein (3074-TH). This combination works very well for detecting the TSP1 in human serum and plasma samples.
