Human Thymosin  beta 4 Antibody

Catalog # Availability Size / Price Qty
AF6796
AF6796-SP
Human Thymosin  beta 4 Antibody in Data
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Human Thymosin  beta 4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Thymosin beta 4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Thymosin beta 10 and rhThymosin beta 16 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Thymosin beta 4
Ser2-Ser44
Accession # P62328
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Thymosin  beta 4 antibody by Western Blot. View Larger

Detection of Human Thymosin beta 4 by Western Blot. Western blot shows lysates of human peripheral blood lymphocytes (PBL), HL-60 human acute promyelocytic leukemia cell line, and human mature dendritic cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Thymosin beta 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6796) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thymosin beta 4 at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Thymosin  beta 4 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Thymosin beta 4 in HeLa Human Cell Line. Thymosin beta 4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Thymosin beta 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6796) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thymosin beta 4

Thymosin beta 4 (T beta 4; also TB4X and Fx) is a 5.0 kDa member of the beta -thymosin family of molecules. Members of this family range from 41-44 amino acids (aa) in length, and possess an isoelectric point that lies between pH 4.0-7.0 ( alpha -thymosins have values less than 4.0). Multiple cell types produce T beta 4, either constitutively, or after stimulation. They include platelets, endothelial cells, neutrophils, astrocytes and macrophages. T beta 4 is both a secreted and intracellular molecule. The secreted form contributes to wound healing and angiogenesis, and may act on ATPase. Intracellularly, it forms a 1:1 complex with G-actin and blocks F-actin polymerization. This regulates the availablility of actin monomers for filament formation and subsequent cell migration. Mature human T beta 4 is 43 aa in length (aa 2-44). It contains an actin-binding site (aa 17-23), one acetylated Ser and five acetylated lysines (4; 12; 26; 32; 39) and one phosphorylation site at Thr23. T beta 4 undergoes proteolytic processing to generate an N-terminal acetylated peptide (aa 2-5: SerAspLysPro). Mature human T beta 4 is identical to mouse T beta 14 in aa sequence, and it shares 74% aa identity with its human family member T beta 10.

Entrez Gene IDs
7114 (Human)
Alternate Names
PTMB4; T beta-4; TB4; TB4X; TB4Xbeta 4, X chromosome; THYB4; Thymosin beta 4; thymosin beta 4, X-linked; thymosin beta-4; TMSB4; TMSB4X

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