Human TNF-alpha DuoSet ELISA

(131 citations)
(2 Reviews)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Sample Type & Volume Required
    100 µL
  • Range
    15.60 - 1,000 pg/mL
  • Sufficient Materials
    For five or fifteen 96-well plates*
  • Specificity
    Please see the product datasheet
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Ancillary Reagent Kit Available

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human TNF-alpha. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 


Product Features
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
Kit Content
  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

 

  

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TNF-alpha

Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

  • Entrez Gene IDs:
    7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit);
  • Long Name:
    Tumor Necrosis Factor alpha
  • Aliases:
    APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

131 Citations: Showing 1 - 10
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Species
Sample Type
  1. Interleukin-1 primes human mesenchymal stem cells towards an anti-inflammatory and pro-trophic phenotype in vitro
    Authors: E Redondo-Ca, C Cunningham, J Miller, L Martuscell, S Aoulad-Ali, NJ Rothwell, CM Kielty, SM Allan, E Pinteaux
    Stem Cell Res Ther, 2017;8(1):79.
    Species: Human
    Sample Type: Cell Culture Supernates
  2. TSG-6 Downregulates IFN-Alpha and TNF-Alpha Expression by Suppressing IRF7 Phosphorylation in Human Plasmacytoid Dendritic Cells
    Authors: L Kui, GC Chan, PP Lee
    Mediators Inflamm., 2017;2017(0):7462945.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. Changes in peripheral immune cell numbers and functions in octogenarian walkers - an acute exercise study
    Authors: KS van der Ge, Q Wang, TM Eijsvogels, HJ Koenen, I Joosten, E Brouwer, MT Hopman, JF Jacobs, AM Boots
    Immun Ageing, 2017;14(0):5.
    Species: Human
    Sample Type: Cell Culture Supernates
  4. On the cytokine/chemokine network during Plasmodium vivax malaria: new insights to understand the disease
    Authors: NS Hojo-Souza, DB Pereira, FS de Souza, TA de Oliveir, MS Cardoso, MS Tada, GM Zanini, DC Bartholome, RT Fujiwara, LL Bueno
    Malar. J, 2017;16(1):42.
    Species: Human
    Sample Type: Plasma
  5. Vitamin D both facilitates and attenuates the cellular response to lipopolysaccharide
    Authors: L Chen, MS Eapen, GR Zosky
    Sci Rep, 2017;7(0):45172.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. Pre- and post-surgical evaluation of the inflammatory response in patients with aortic stenosis treated with different types of prosthesis
    Authors: ME Soto, JL Salas, J Vargas-Bar, R Marquez, A Rodriguez-, R Bojalil-Pa, I Pérez-Torr, V Guarner-La
    BMC Cardiovasc Disord, 2017;17(1):100.
    Species: Human
    Sample Type: Serum
  7. CLEC5A is a critical receptor in innate immunity against Listeria infection
    Authors: ST Chen, FJ Li, TY Hsu, SM Liang, YC Yeh, WY Liao, TY Chou, NJ Chen, M Hsiao, WB Yang, SL Hsieh
    Nat Commun, 2017;8(1):299.
    Species: Human
    Sample Type: Cell Culture Supernates
  8. Vomocytosis of live pathogens from macrophages is regulated by the atypical MAP kinase ERK5
    Authors: AS Gilbert, PI Seoane, P Sephton-Cl, A Bojarczuk, R Hotham, E Giurisato, AR Sarhan, A Hillen, GV Velde, NS Gray, DR Alessi, DL Cunningham, C Tournier, SA Johnston, RC May
    Sci Adv, 2017;3(8):e1700898.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. SP and IL-33 together markedly enhance TNF synthesis and secretion from human mast cells mediated by the interaction of their receptors
    Authors: A Taracanova, M Alevizos, A Karagkouni, Z Weng, E Norwitz, P Conti, SE Leeman, TC Theoharide
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  10. Allostimulatory capacity of conditionally immortalized proximal tubule cell lines for bioartificial kidney application
    Authors: M Mihajlovic, LP van den He, JG Hoenderop, J Jansen, MJ Wilmer, AJF Westheim, WA Allebes, D Stamatiali, LB Hilbrands, R Masereeuw
    Sci Rep, 2017;7(1):7103.
    Species: Human
    Sample Type: Cell Culture Supernates
  11. CD36-Mediated Uptake of Surfactant Lipids by Human Macrophages Promotes Intracellular Growth of Mycobacterium tuberculosis
    J. Immunol., 2016;197(12):4727-4735.
    Species: Human
    Sample Type: cell culture supernatants
  12. Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-?
    Aging Cell, 2016;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  13. Rational design of a Kv1.3 channel-blocking antibody as a selective immunosuppressant
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  14. 17-oxo-DHA displays additive anti-inflammatory effects with fluticasone propionate and inhibits the NLRP3 inflammasome
    Sci Rep, 2016;6(0):37625.
    Species: Human
    Sample Type: Cell Culture Supernates
  15. Reduced systemic and mycobacterial antigen-stimulated concentrations of IL-1? and IL-18 in tuberculous lymphadenitis
    Cytokine, 2016;90(0):66-72.
    Species: Human
    Sample Type: Whole Blood Supernates
  16. Temperature responsive porous silicon nanoparticles for cancer therapy - spatiotemporal triggering through infrared and radiofrequency electromagnetic heating
    J Control Release, 2016;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  17. Serum from Varicose Patients Induces Senescence-Related Dysfunction of Vascular Endothelium Generating Local and Systemic Proinflammatory Conditions
    Oxid Med Cell Longev, 2016;2016(0):2069290.
    Species: Human
    Sample Type: Serum
  18. Amyloid precursor protein mediated changes in intestinal epithelial phenotype in vitro.
    Authors: Puig K, Manocha G, Combs C
    PLoS ONE, 2016;10(3):e0119534.
    Species: Human
    Sample Type: Cell Culture Supernates
  19. vIL-10-overexpressing human MSCs modulate na�ve and activated T lymphocytes following induction of collagenase-induced osteoarthritis
    Stem Cell Res Ther, 2016;7(1):74.
    Species: Human
    Sample Type: Cell Culture Supernates
  20. Characterization of the MMP/TIMP Imbalance and Collagen Production Induced by IL-1? or TNF-? Release from Human Hepatic Stellate Cells
    Authors: S Robert, T Gicquel, A Bodin, V Lagente, E Boichot
    PLoS ONE, 2016;11(4):e0153118.
    Species: Human
    Sample Type: Cell Culture Supernates
  21. Genetic polymorphisms of cell adhesion molecules in Behcet's disease in a Chinese Han population
    Authors: M Zheng, L Zhang, H Yu, J Hu, Q Cao, G Huang, Y Huang, G Yuan, A Kijlstra, P Yang
    Sci Rep, 2016;6(0):24974.
    Species: Human
    Sample Type: Cell Culture Supernates
  22. Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation
    Authors: A Malecka, Q Wang, S Shah, RV Sutavani, I Spendlove, JM Ramage, J Greensmith, HA Franks, MJ Gough, A Saalbach, PM Patel, AM Jackson
    J Leukoc Biol, 2016;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  23. New Anti-Inflammatory Metabolites by Microbial Transformation of Medrysone
    Authors: S Bano, AT Wahab, S Yousuf, A Jabeen, MA Mesaik, AU Rahman, MI Choudhary
    PLoS ONE, 2016;11(4):e0153951.
    Species: Human
    Sample Type: Cell Culture Supernates
  24. Analysis of receptor tyrosine kinase genetics identifies two novel risk loci in GAS6 and PROS1 in Beh�et's disease
    Sci Rep, 2016;6(0):26662.
    Species: Human
    Sample Type: Cell Culture Supernates
  25. Bortezomib treatment sensitizes oncolytic HSV-1 treated tumors to NK cell immunotherapy
    Clin Cancer Res, 2016;0(0):.
    Species: Mouse
    Sample Type: Serum
  26. Soluble alpha-enolase activates monocytes by CD14-dependent TLR4 signalling pathway and exhibits a dual function
    Authors: C Guillou, M Fréret, E Fondard, C Derambure, G Avenel, ML Golinski, M Verdet, O Boyer, F Caillot, P Musette, T Lequerré, O Vittecoq
    Sci Rep, 2016;6(0):23796.
    Species: Human
    Sample Type: Cell Culture Supernates
  27. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes
    Sci Rep, 2016;6(0):34310.
    Species: Human
    Sample Type: Whole Cells
  28. Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability
    Sci Rep, 2016;6(0):39035.
    Species: Human
    Sample Type: Cell Culture Supernates
  29. Transcriptomic Analyses of the Biological Effects of Airborne PM2.5 Exposure on Human Bronchial Epithelial Cells.
    Authors: Zhou Z, Liu Y, Duan F, Qin M, Wu F, Sheng W, Yang L, Liu J, He K
    PLoS ONE, 2015;10(9):e0138267.
    Species: Human
    Sample Type: Cell Culture Supernates
  30. Exposure to diesel exhaust particle extracts (DEPe) impairs some polarization markers and functions of human macrophages through activation of AhR and Nrf2.
    Authors: Jaguin M, Fardel O, Lecureur V
    PLoS ONE, 2015;10(2):e0116560.
    Species: Human
    Sample Type: Cell Culture Supernates
  31. In vitro progesterone modulation on bacterial endotoxin-induced production of IL-1beta, TNFalpha, IL-6, IL-8, IL-10, MIP-1alpha, and MMP-9 in pre-labor human term placenta.
    Authors: Garcia-Ruiz G, Flores-Espinosa P, Preciado-Martinez E, Bermejo-Martinez L, Espejel-Nunez A, Estrada-Gutierrez G, Maida-Claros R, Flores-Pliego A, Zaga-Clavellina V
    Reprod Biol Endocrinol, 2015;13(0):115.
    Species: Human
    Sample Type: Cell Culture Supernates
  32. Interleukin-23 Receptor Gene Polymorphism May Enhance Expression of the IL-23 Receptor, IL-17, TNF-alpha and IL-6 in Behcet's Disease.
    Authors: Jiang Z, Hennein L, Tao Y, Tao L
    PLoS ONE, 2015;10(7):e0134632.
    Species: Human
    Sample Type: Cell Culture Supernates
  33. Sensor Function for Butyrophilin 3A1 in Prenyl Pyrophosphate Stimulation of Human Vgamma2Vdelta2 T Cells.
    Authors: Wang H, Morita C
    J Immunol, 2015;195(10):4583-94.
    Species: Human
    Sample Type: Cell Culture Supernates
  34. Vpr Enhances Tumor Necrosis Factor Production by HIV-1-Infected T Cells.
    Authors: Roesch F, Richard L, Rua R, Porrot F, Casartelli N, Schwartz O
    J Virol, 2015;89(23):12118-30.
    Species: Human
    Sample Type: Cell Culture Supernates
  35. Innate immune response to Streptococcus pyogenes depends on the combined activation of TLR13 and TLR2.
    Authors: Fieber C, Janos M, Koestler T, Gratz N, Li X, Castiglia V, Aberle M, Sauert M, Wegner M, Alexopoulou L, Kirschning C, Chen Z, von Haeseler A, Kovarik P
    PLoS ONE, 2015;10(3):e0119727.
    Species: Human
    Sample Type: Cell Culture Supernates
  36. An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells.
    Authors: Campia I, Buondonno I, Castella B, Rolando B, Kopecka J, Gazzano E, Ghigo D, Riganti C
    PLoS ONE, 2015;10(5):e0126159.
    Species: Human
    Sample Type: Cell Culture Supernates
  37. Characterization of host and microbial determinants in individuals with latent tuberculosis infection using a human granuloma model.
    Authors: Guirado E, Mbawuike U, Keiser T, Arcos J, Azad A, Wang S, Schlesinger L
    MBio, 2015;6(1):e02537-14.
    Species: Human
    Sample Type: cell supernates
  38. Phenotypic profiling of CD8(+) T cells during Plasmodium vivax blood-stage infection.
    Authors: Hojo-Souza N, Pereira D, Passos L, Gazzinelli-Guimaraes P, Cardoso M, Tada M, Zanini G, Bartholomeu D, Fujiwara R, Bueno L
    BMC Infect Dis, 2015;15(0):35.
    Species: Human
    Sample Type: Plasma
  39. SRSF1 facilitates cytosolic DNA-induced production of type I interferons recognized by RIG-I.
    Authors: Xue F, Li X, Zhao X, Wang L, Liu M, Shi R, Zheng J
    PLoS ONE, 2015;10(2):e0115354.
    Species: Human
    Sample Type: Cell Culture Supernates
  40. Macrophage activation in acute exacerbation of idiopathic pulmonary fibrosis.
    Authors: Schupp J, Binder H, Jager B, Cillis G, Zissel G, Muller-Quernheim J, Prasse A
    PLoS ONE, 2015;10(1):e0116775.
    Species: Human
    Sample Type: BALFf
  41. Phagocytosis-dependent activation of a TLR9-BTK-calcineurin-NFAT pathway co-ordinates innate immunity to Aspergillus fumigatus.
    Authors: Herbst S, Shah A, Mazon Moya M, Marzola V, Jensen B, Reed A, Birrell M, Saijo S, Mostowy S, Shaunak S, Armstrong-James D
    EMBO Mol Med, 2015;7(3):240-58.
    Species: Human
    Sample Type: Cell Culture Supernates
  42. Sourcing of an alternative pericyte-like cell type from peripheral blood in clinically relevant numbers for therapeutic angiogenic applications.
    Authors: Blocki A, Wang Y, Koch M, Goralczyk A, Beyer S, Agarwal N, Lee M, Moonshi S, Dewavrin J, Peh P, Schwarz H, Bhakoo K, Raghunath M
    Mol Ther, 2015;23(3):510-22.
    Species: Human
    Sample Type: Cell Culture Supernates
  43. The small GTPase Arf6 is essential for the Tram/Trif pathway in TLR4 signaling.
    Authors: Van Acker T, Eyckerman S, Vande Walle L, Gerlo S, Goethals M, Lamkanfi M, Bovijn C, Tavernier J, Peelman F
    J Biol Chem, 2014;289(3):1364-76.
  44. Separately or combined, LukG/LukH is functionally unique compared to other staphylococcal bicomponent leukotoxins.
    Authors: Yanai M, Rocha M, Matolek A, Chintalacharuvu A, Taira Y, Chintalacharuvu K, Beenhouwer D
    PLoS ONE, 2014;9(2):e89308.
    Species: Human
    Sample Type: Cell Culture Supernates
  45. TRAF5 and TRAF3IP2 gene polymorphisms are associated with Behcet's disease and Vogt-Koyanagi-Harada syndrome: a case-control study.
    Authors: Xiang Q, Chen L, Hou S, Fang J, Zhou Y, Bai L, Liu Y, Kijlstra A, Yang P
    PLoS ONE, 2014;9(1):e84214.
    Species: Human
    Sample Type: Cell Culture Supernates
  46. CD137 ligand signalling induces differentiation of primary acute myeloid leukaemia cells.
    Authors: Cheng K, Wong S, Linn Y, Ho L, Chng W, Schwarz H
    Br J Haematol, 2014;165(1):134-44.
    Species: Human
    Sample Type: Cell Culture Supernates
  47. The aryl hydrocarbon receptor is functionally upregulated early in the course ofvhuman T-cell activation.
    Authors: Prigent L, Robineau M, Jouneau S, Morzadec C, Louarn L, Vernhet L, Fardel O, Sparfel L
    Eur J Immunol, 2014;44(5):1330-40.
    Species: Human
    Sample Type: Cell Culture Supernates
  48. Low adiposity during early infancy is associated with a low risk for developing dengue hemorrhagic fever: a preliminary model.
    Authors: Libraty D, Zhang L, Woda M, Giaya K, Kathivu C, Acosta L, Tallo V, Segubre-Mercado E, Bautista A, Obcena A, Brion J, Capeding R
    PLoS ONE, 2014;9(2):e88944.
    Species: Human
    Sample Type: Cell Culture Supernates
  49. Technical advance: live-imaging analysis of human dendritic cell migrating behavior under the influence of immune-stimulating reagents in an organotypic model of lung.
    Authors: Nguyen Hoang A, Chen P, Bjornfot S, Hogstrand K, Lock J, Grandien A, Coles M, Svensson M
    J Leukoc Biol, 2014;96(3):481-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  50. Activin A as a mediator of NK-dendritic cell functional interactions.
    Authors: Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, Sozzani S
    J Immunol, 2014;192(3):1241-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  51. Immunoepidemiological profiling of onchocerciasis patients reveals associations with microfilaria loads and ivermectin intake on both individual and community levels.
    Authors: Arndts, Kathrin, Specht, Sabine, Debrah, Alexande, Tamarozzi, Francesc, Klarmann Schulz, Ute, Mand, Sabine, Batsa, Linda, Kwarteng, Alexande, Taylor, Mark, Adjei, Ohene, Martin, Coralie, Layland, Laura E, Hoerauf, Achim
    PLoS Negl Trop Dis, 2014;8(2):e2679.
    Species: Human
    Sample Type: Cell Culture Supernates
  52. Linkage of inflammation and oxidative stress via release of glutathionylated peroxiredoxin-2, which acts as a danger signal.
    Authors: Salzano S, Checconi P, Hanschmann E, Lillig C, Bowler L, Chan P, Vaudry D, Mengozzi M, Coppo L, Sacre S, Atkuri K, Sahaf B, Herzenberg L, Herzenberg L, Mullen L, Ghezzi P
    Proc Natl Acad Sci U S A, 2014;111(33):12157-62.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  53. Increased prevalence of Methanosphaera stadtmanae in inflammatory bowel diseases.
    Authors: Blais Lecours P, Marsolais D, Cormier Y, Berberi M, Hache C, Bourdages R, Duchaine C
    PLoS ONE, 2014;9(2):e87734.
    Species: Human
    Sample Type: Cell Culture Supernates
  54. Neisseria gonorrhoeae modulates iron-limiting innate immune defenses in macrophages.
    Authors: Zughaier, Susu M, Kandler, Justin L, Shafer, William
    PLoS ONE, 2014;9(1):e87688.
    Species: Human
    Sample Type: Cell Culture Supernates
  55. Activated factor X signaling via protease-activated receptor 2 suppresses pro-inflammatory cytokine production from lipopolysaccharide-stimulated myeloid cells.
    Authors: Gleeson E, O'Donnell J, Hams E, Ni Ainle F, Kenny B, Fallon P, Preston R
    Haematologica, 2014;99(1):185-93.
    Species: Human
    Sample Type: Cell Culture Supernates
  56. A functional variant of PTPN22 confers risk for Vogt-Koyanagi-Harada syndrome but not for ankylosing spondylitis.
    Authors: Zhang Q, Qi J, Hou S, DU L, Yu H, Cao Q, Zhou Y, Liao D, Kijlstra A, Yang P
    PLoS ONE, 2014;9(5):e96943.
    Species: Human
    Sample Type: Cell Culture Supernates
  57. Anti-inflammatory effects of green soybean extract irradiated with visible light.
    Authors: Tanaka K, Ohgo Y, Katayanagi Y, Yasui K, Hiramoto S, Ikemoto H, Nakata Y, Miyoshi N, Isemura M, Ohashi N, Imai S
    Sci Rep, 2014;4(0):4732.
    Species: Human
    Sample Type: Cell Culture Supernates
  58. Engagement of SLAMF2/CD48 prolongs the time frame of effective T cell activation by supporting mature dendritic cell survival.
    Authors: Kis-Toth K, Tsokos G
    J Immunol, 2014;192(9):4436-42.
    Species: Human
    Sample Type: Cell Culture Supernates
  59. Identification of the flagellin glycosylation system in Burkholderia cenocepacia and the contribution of glycosylated flagellin to evasion of human innate immune responses.
    Authors: Hanuszkiewicz A, Pittock P, Humphries F, Moll H, Rosales A, Molinaro A, Moynagh P, Lajoie G, Valvano M
    J Biol Chem, 2014;289(27):19231-44.
    Species: Human
    Sample Type: Cell Culture Supernates
  60. Smac mimetics and innate immune stimuli synergize to promote tumor death.
    Authors: Beug S, Tang V, Lacasse E, Cheung H, Beauregard C, Brun J, Nuyens J, Earl N, St-Jean M, Holbrook J, Dastidar H, Mahoney D, Ilkow C, Le Boeuf F, Bell J, Korneluk R
    Nat Biotechnol, 2014;32(2):182-90.
    Species: Human
    Sample Type: Cell Culture Supernates
  61. Fcgamma receptor-induced soluble vascular endothelial growth factor receptor-1 (VEGFR-1) production inhibits angiogenesis and enhances efficacy of anti-tumor antibodies.
    Authors: Justiniano S, Elavazhagan S, Fatehchand K, Shah P, Mehta P, Roda J, Mo X, Cheney C, Hertlein E, Eubank T, Marsh C, Muthusamy N, Butchar J, Byrd J, Tridandapani S
    J Biol Chem, 2013;288(37):26800-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  62. S100A12 suppresses pro-inflammatory, but not pro-thrombotic functions of serum amyloid A.
    Authors: Chung Y, Goyette J, Tedla N, Hsu K, Geczy C
    PLoS ONE, 2013;8(4):e62372.
  63. Leptin up-regulates TLR2 in human monocytes.
    Authors: Jaedicke K, Roythorne A, Padget K, Todryk S, Preshaw P, Taylor J
    J Leukoc Biol, 2013;93(4):561-71.
    Species: Human
    Sample Type: Cell Culture Supernates
  64. Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling.
    Authors: Moh M, Lorenzini P, Gullo C, Schwarz H
    FASEB J, 2013;27(8):2957-66.
    Species: Human
    Sample Type: Cell Culture Supernates
  65. Association of TLR2 gene polymorphisms with ocular Behcet's disease in a Chinese Han population.
    Authors: Fang J, Hu R, Hou S, Ye Z, Xiang Q, Qi J, Zhou Y, Kijlstra A, Yang P
    Invest Ophthalmol Vis Sci, 2013;54(13):8384-92.
    Species: Human
    Sample Type: Cell Culture Supernates
  66. Human neutrophil peptide-1 (HNP-1): a new anti-leishmanial drug candidate.
    Authors: Dabirian S, Taslimi Y, Zahedifard F, Gholami E, Doustdari F, Motamedirad M, Khatami S, Azadmanesh K, Nylen S, Rafati S
    PLoS Negl Trop Dis, 2013;7(10):e2491.
    Species: Human
    Sample Type: Cell Culture Supernates
  67. Identification of binding sites for myeloid differentiation primary response gene 88 (MyD88) and Toll-like receptor 4 in MyD88 adapter-like (Mal).
    Authors: Bovijn C, Desmet A, Uyttendaele I, Van Acker T, Tavernier J, Peelman F
    J Biol Chem, 2013;288(17):12054-66.
    Species: Human
    Sample Type: Cell Culture Supernates
  68. Glycosylation in a mammalian expression system is critical for the production of functionally active leukocyte immunoglobulin-like receptor A3 protein.
    Authors: Lee T, Mitchell A, Liu Lau S, An H, Rajeaskariah P, Wasinger V, Raftery M, Bryant K, Tedla N
    J Biol Chem, 2013;288(46):32873-85.
    Species: Human
    Sample Type: cell supernates
  69. Survey of innate immune responses to Burkholderia pseudomallei in human blood identifies a central role for lipopolysaccharide.
    Authors: Chantratita N, Tandhavanant S, Myers N, Seal S, Arayawichanont A, Kliangsa-Ad A, Hittle L, Ernst R, Emond M, Wurfel M, Day N, Peacock S, West T
    PLoS ONE, 2013;8(11):e81617.
    Species: Human
    Sample Type: Cell Culture Supernates
  70. Thrombin-cleaved fragments of osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage.
    Authors: Yamaguchi Y, Shao Z, Sharif S, Du X, Myles T, Merchant M, Harsh G, Glantz M, Recht L, Morser J, Leung L
    J Biol Chem, 2013;288(5):3097-111.
    Species: Human
    Sample Type: CSF
  71. Effects of high-dose cholecalciferol on serum markers of inflammation and immunity in patients with early chronic kidney disease.
    Authors: Alvarez J, Zughaier S, Law J, Hao L, Wasse H, Ziegler T, Tangpricha V
    Eur J Clin Nutr, 2013;67(3):264-9.
    Species: Human
    Sample Type: Serum
  72. Characterization and function of the human macrophage dopaminergic system: implications for CNS disease and drug abuse.
    J Neuroinflammation, 2012;9(0):203.
    Species: Human
    Sample Type: Cell Culture Supernates
  73. Elevated adaptive immune responses are associated with latent infections of Wuchereria bancrofti.
    Authors: Arndts K, Deininger S, Specht S, Klarmann U, Mand S, Adjobimey T, Debrah AY, Batsa L, Kwarteng A, Epp C, Taylor M, Adjei O, Layland LE, Hoerauf A
    PLoS Negl Trop Dis, 2012;6(4):e1611.
    Species: Human
    Sample Type: Cell Culture Supernates
  74. Age-related changes in expression and function of Toll-like receptors in human skin.
    Authors: Iram N, Mildner M, Prior M, Petzelbauer P, Fiala C, Hacker S, Schoppl A, Tschachler E, Elbe-Burger A
    Development, 2012;139(22):4210-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  75. Impact of vitamin D supplementation on markers of inflammation in adults with cystic fibrosis hospitalized for a pulmonary exacerbation.
    Eur J Clin Nutr, 2012;66(9):1072-4.
    Species: Human
    Sample Type: Plasma
  76. Trappin-2/elafin modulate innate immune responses of human endometrial epithelial cells to PolyI:C.
    Authors: Drannik AG, Nag K, Yao XD, Henrick BM, Sallenave JM, Rosenthal KL
    PLoS ONE, 2012;7(4):e35866.
    Species: Human
    Sample Type: Cell Culture Supernates
  77. Anti-Tumour Effects of a Specific Anti-ADAM17 Antibody in an Ovarian Cancer Model In Vivo.
    Authors: Richards FM, Tape CJ, Jodrell DI, Murphy G
    PLoS ONE, 2012;7(7):e40597.
    Species: Human
    Sample Type: Tissue Homogenates
  78. TRAIL induces necroptosis involving RIPK1/RIPK3-dependent PARP-1 activation.
    Authors: Jouan-Lanhouet S, Arshad M, Piquet-Pellorce C, Martin-Chouly C, Le Moigne-Muller G, Van Herreweghe F, Takahashi N, Sergent O, Lagadic-Gossmann D, Vandenabeele P, Samson M, Dimanche-Boitrel M
    Cell Death Differ, 2012;19(12):2003-14.
    Species: Human
    Sample Type: Cell Culture Supernates
  79. Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12).
    Authors: Suh HS, Choi N, Tarassishin L, Lee SC
    PLoS ONE, 2012;7(4):e35115.
    Species: Human
    Sample Type: Cell Culture Supernates
  80. Transglutaminase is essential for IgA nephropathy development acting through IgA receptors.
    Authors: Berthelot L, Papista C, Maciel TT, Biarnes-Pelicot M, Tissandie E, Wang PH, Tamouza H, Jamin A, Bex-Coudrat J, Gestin A, Boumediene A, Arcos-Fajardo M, England P, Pillebout E, Walker F, Daugas E, Vrtosvnik F, Flamant M, Benhamou M, Cogne M, Moura IC, Monteiro RC
    J. Exp. Med., 2012;209(4):793-806.
    Species: Human
    Sample Type: Cell Culture Supernates
  81. Plasma levels of procalcitonin and eight additional inflammatory molecules in febrile neutropenic patients.
    Authors: Neuenschwander LC, Bittencourt H, Ribeiro AF, Teixeira AL, Teixeira MM, Teixeira JC, Nobre V
    Clinics (Sao Paulo), 2011;66(10):1699-705.
    Species: Human
    Sample Type: Plasma
  82. Inhibition of IL-32 activation by alpha-1 antitrypsin suppresses alloreactivity and increases survival in an allogeneic murine marrow transplantation model.
    Authors: Marcondes AM, Li X, Tabellini L, Bartenstein M, Kabacka J, Sale GE, Hansen JA, Dinarello CA, Deeg HJ
    Blood, 2011;118(18):5031-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  83. Monocytic microparticles activate endothelial cells in an IL-1{beta}-dependent manner.
    Authors: Wang JG, Williams JC, Davis BK, Jacobson K, Doerschuk CM, Ting JP, Mackman N
    Blood, 2011;118(8):2366-74.
    Species: Human
    Sample Type: Cell Lysates
  84. Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques.
    Authors: Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D
    Thromb. Haemost., 2011;107(1):140-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  85. Adipokine resistin predicts anti-inflammatory effect of glucocorticoids in asthma.
    Authors: Leivo-Korpela S, Lehtimaki L, Vuolteenaho K, Nieminen R, Kankaanranta H, Saarelainen S, Moilanen E
    J Inflamm (Lond), 2011;8(0):12.
    Species: Human
    Sample Type: Cell Culture Supernates
  86. Reactive oxygen species-independent activation of the IL-1beta inflammasome in cells from patients with chronic granulomatous disease.
    Authors: van de Veerdonk FL, Smeekens SP, Joosten LA, Kullberg BJ, Dinarello CA, Van der Meer JW, Netea MG
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(7):3030-3.
    Species: Human
    Sample Type: Cell Culture Supernates
  87. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010;184(9):4880-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  88. Activation of platelets by Aspergillus fumigatus and potential role of platelets in the immunopathogenesis of Aspergillosis.
    Authors: Rodland EK, Ueland T, Pedersen TM, Halvorsen B, Muller F, Aukrust P, Froland SS
    Infect. Immun., 2010;78(3):1269-75.
    Species: Human
    Sample Type: Cell Culture Supernates
  89. KR-003048, a potent, orally active inhibitor of p38 mitogen-activated protein kinase.
    Authors: Montalban AG, Boman E, Chang CD, Ceide SC, Dahl R, Dalesandro D, Delaet NG, Erb E, Ernst J, Gibbs A, Kahl J, Kessler L, Lundstrom J, Miller S, Nakanishi H, Roberts E, Saiah E, Sullivan R, Wang Z, Larson CJ
    Eur. J. Pharmacol., 2010;632(1):93-102.
    Species: Human
    Sample Type: Cell Culture Supernates
  90. Reduced ex vivo interleukin-6 production by dietary fish oil is not modified by linoleic acid intake in healthy men.
    Authors: Damsgaard CT, Lauritzen L, Calder PC, Kjaer TR, Frokiaer H
    J. Nutr., 2009;139(7):1410-4.
    Species: Human
    Sample Type: Cell Culture Supernates
  91. Heat shock protein 96 is elevated in rheumatoid arthritis and activates macrophages primarily via TLR2 signaling.
    Authors: Huang QQ, Sobkoviak R, Jockheck-Clark AR, Shi B, Mandelin AM, Tak PP, Haines GK, Nicchitta CV, Pope RM
    J. Immunol., 2009;182(8):4965-73.
    Species: Human
    Sample Type: Cell Culture Supernates
  92. Interferon-beta treatment in multiple sclerosis attenuates inflammatory gene expression through inducible activity of the phosphatase SHP-1.
    Authors: Christophi GP, Panos M, Hudson CA, Tsikkou C, Mihai C, Mejico LJ, Jubelt B, Massa PT
    Clin. Immunol., 2009;133(1):27-44.
    Species: Human
    Sample Type: Plasma
  93. Helminth antigens modulate immune responses in cells from multiple sclerosis patients through TLR2-dependent mechanisms.
    Authors: Correale J, Farez M
    J. Immunol., 2009;183(9):5999-6012.
    Species: Human
    Sample Type: Cell Culture Supernates
  94. Survival and migration of human dendritic cells are regulated by an IFN-alpha-inducible Axl/Gas6 pathway.
    Authors: Scutera S, Fraone T, Musso T, Cappello P, Rossi S, Pierobon D, Orinska Z, Paus R, Bulfone-Paus S, Giovarelli M
    J. Immunol., 2009;183(5):3004-13.
    Species: Human
    Sample Type: Cell Culture Supernates
  95. The role of RGD-tagged helical rosette nanotubes in the induction of inflammation and apoptosis in human lung adenocarcinoma cells through the P38 MAPK pathway.
    Authors: Suri SS, Rakotondradany F, Myles AJ, Fenniri H, Singh B
    Biomaterials, 2009;30(17):3084-90.
    Species: Human
    Sample Type: Cell Culture Supernates
  96. Inorganic arsenic activates reduced NADPH oxidase in human primary macrophages through a Rho kinase/p38 kinase pathway.
    Authors: Lemarie A, Bourdonnay E, Morzadec C, Fardel O, Vernhet L
    J. Immunol., 2008;180(9):6010-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  97. Fungal proteases induce Th2 polarization through limited dendritic cell maturation and reduced production of IL-12.
    Authors: Lamhamedi-Cherradi SE, Martin RE, Ito T, Kheradmand F, Corry DB, Liu YJ, Moyle M
    J. Immunol., 2008;180(9):6000-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  98. Impact of tobacco-smoke on key signaling pathways in the innate immune response in lung macrophages.
    Authors: Birrell MA, Wong S, Catley MC, Belvisi MG
    J. Cell. Physiol., 2008;214(1):27-37.
    Species: Human
    Sample Type: Cell Culture Supernates
  99. Helminth infections associated with multiple sclerosis induce regulatory B cells.
    Authors: Correale J, Farez M, Razzitte G
    Ann. Neurol., 2008;64(2):187-99.
    Species: Human
    Sample Type: Cell Culture Supernates
  100. Divergent effects of hypoxia on dendritic cell functions.
    Authors: Mancino A, Schioppa T, Larghi P, Pasqualini F, Nebuloni M, Chen IH, Sozzani S, Austyn JM, Mantovani A, Sica A
    Blood, 2008;112(9):3723-34.
    Species: Human
    Sample Type: Cell Culture Supernates
  101. Early cytokine responses after percutaneous magnetic resonance imaging guided laser thermoablation of malignant liver tumors.
    Authors: Kallio R, Sequeiros R, Surcel HM, Ohtonen P, Kiviniemi H, Syrjala H
    Cytokine, 2007;34(5):278-83.
    Species: Human
    Sample Type: Serum
  102. Cytokine response to strenuous exercise in athletes and non-athletes--an adaptive response.
    Authors: Gokhale R, Chandrashekara S, Vasanthakumar KC
    Cytokine, 2007;40(2):123-7.
    Species: Human
    Sample Type: Plasma
  103. The differential effect of Eriobotrya japonica hydrophilic leaf extract on cytokines production and modulation.
    Authors: Matalka KZ, Ali D, Khawad AE, Qa'dan F
    Cytokine, 2007;40(3):235-40.
    Species: Human
    Sample Type: Cell Culture Supernates
  104. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007;117(12):3664-3672.
    Species: Human
    Sample Type: Cell Culture Supernates
  105. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007;110(13):4319-30.
    Species: Human
    Sample Type: Cell Culture Supernates
  106. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
    Authors: Skibinski G, Elborn JS, Ennis M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76.
    Species: Human
    Sample Type: Cell Culture Supernates
  107. Novel role for the liver X nuclear receptor in the suppression of lung inflammatory responses.
    Authors: Birrell MA, Catley MC, Hardaker E, Wong S, Willson TM, McCluskie K, Leonard T, Farrow SN, Collins JL, Haj-Yahia S, Belvisi MG
    J. Biol. Chem., 2007;282(44):31882-90.
    Species: Human
    Sample Type: Cell Culture Supernates
  108. Sirtuin regulates cigarette smoke-induced proinflammatory mediator release via RelA/p65 NF-kappaB in macrophages in vitro and in rat lungs in vivo: implications for chronic inflammation and aging.
    Authors: Yang SR, Wright J, Bauter M, Seweryniak K, Kode A, Rahman I
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(2):L567-76.
    Species: Human
    Sample Type: Cell Culture Supernates
  109. Role of matrix metalloproteinases in the inflammatory response in human airway cell-based assays and in rodent models of airway disease.
    Authors: Birrell MA, Wong S, Dekkak A, De Alba J, Haj-Yahia S, Belvisi MG
    J. Pharmacol. Exp. Ther., 2006;318(2):741-50.
    Species: Human
    Sample Type: Cell Culture Supernates
  110. Splice variants of human FOXP3 are functional inhibitors of human CD4+ T-cell activation.
    Authors: Smith EL, Finney HM, Nesbitt AM, Ramsdell F, Robinson MK
    Immunology, 2006;119(2):203-11.
    Species: Human
    Sample Type: Cell Culture Supernates
  111. Dynamic changes of apoptosis-inducing ligands and Th1/Th2 like subpopulations in Hantaan virus-induced hemorrhagic fever with renal syndrome.
    Authors: Liu JM, Zhu Y, Xu ZW, Ouyang WM, Wang JP, Liu XS, Cao YX, Li Q, Fang L, Zhuang R, Yang AG, Jin BQ
    Clin. Immunol., 2006;119(3):245-51.
    Species: Human
    Sample Type: Plasma
  112. A combination of E. coli DNA fragments and modified lipopolysaccharides as a cancer immunotherapy.
    Authors: Cho YJ, Ahn BY, Lee NG, Lee DH, Kim DS
    Vaccine, 2006;24(31):5862-71.
    Species: Human
    Sample Type: Cell Culture Supernates
  113. A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression.
    Authors: Macagno A, Molteni M, Rinaldi A
    J. Exp. Med., 2006;203(6):1481-92.
    Species: Human
    Sample Type: Cell Culture Supernates
  114. Transmission of Mycobacterium tuberculosis undetected by tuberculin skin testing.
    Authors: Anderson ST, Williams AJ, Brown JR, Newton SM, Simsova M, Nicol MP, Sebo P, Levin M, Wilkinson RJ, Wilkinson KA
    Am. J. Respir. Crit. Care Med., 2006;173(9):1038-42.
    Species: Human
    Sample Type: Cell Culture Supernates
  115. The direct effects of Toll-like receptor ligands on human NK cell cytokine production and cytotoxicity.
    Authors: Lauzon NM, Mian F, MacKenzie R, Ashkar AA
    Cell. Immunol., 2006;241(2):102-12.
    Species: Human
    Sample Type: Cell Culture Supernates
  116. Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk.
    Authors: LeBouder E, Rey-Nores JE, Raby AC, Affolter M, Vidal K, Thornton CA, Labeta MO
    J. Immunol., 2006;176(6):3742-52.
    Species: Human
    Sample Type: Cell Culture Supernates
  117. Microbial exposure, symptoms and inflammatory mediators in nasal lavage fluid of kitchen and clerical personnel in schools.
    Authors: Lignell U, Meklin T, Putus T, Vepsalainen A, Roponen M, Torvinen E, Reeslev M, Pennanen S, Hirvonen MR, Kalliokoski P, Nevalainen A
    Int J Occup Med Environ Health, 2005;18(2):139-50.
    Species: Human
    Sample Type: nasal lavage
  118. Synergistic stimulation of human monocytes and dendritic cells by Toll-like receptor 4 and NOD1- and NOD2-activating agonists.
    Authors: Fritz JH, Girardin SE, Fitting C, Werts C, Mengin-Lecreulx D, Caroff M, Cavaillon JM, Philpott DJ, Adib-Conquy M
    Eur. J. Immunol., 2005;35(8):2459-70.
    Species: Human
    Sample Type: Cell Culture Supernates
  119. Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma.
    Authors: Pound JD, Batth BK, Johannessen I, Wood K
    J. Immunol., 2005;174(5):3015-23.
    Species: Human
    Sample Type: Cell Culture Supernates
  120. Activation of resting human primary T cells with chimeric receptors: costimulation from CD28, inducible costimulator, CD134, and CD137 in series with signals from the TCR zeta chain.
    Authors: Finney HM, Akbar AN, Lawson AD
    J. Immunol., 2004;172(1):104-13.
    Species: Human
    Sample Type: Cell Culture Supernates
  121. Stimulation of toll-like receptor 2 by Coxiella burnetii is required for macrophage production of pro-inflammatory cytokines and resistance to infection.
    Authors: Zamboni DS, Campos MA, Torrecilhas AC, Kiss K, Samuel JE, Golenbock DT, Lauw FN, Roy CR, Almeida IC, Gazzinelli RT
    J. Biol. Chem., 2004;279(52):54405-15.
    Species: Human
    Sample Type: Cell Culture Supernates
  122. The Toll-like receptor-2/6 agonist macrophage-activating lipopeptide-2 cooperates with IFN-gamma to reverse the Th2 skew in an in vitro allergy model.
    Authors: Weigt H, Muhlradt PF, Larbig M, Krug N, Braun A
    J. Immunol., 2004;172(10):6080-6.
    Species: Human
    Sample Type: Cell Culture Supernates
  123. Association of type 2 cytokines with hepatic fibrosis in human Schistosoma mansoni infection.
    Authors: de Jesus AR, Magalhaes A, Miranda DG, Miranda RG, Araujo MI, de Jesus AA, Silva A, Santana LB, Pearce E, Carvalho EM
    Infect. Immun., 2004;72(6):3391-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  124. IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
    Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
    J. Immunol., 2004;173(2):1463-76.
    Species: Human
    Sample Type: Cell Culture Supernates
  125. Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis.
    Authors: Groh V, Bruhl A, El-Gabalawy H, Nelson JL, Spies T
    Proc. Natl. Acad. Sci. U.S.A., 2003;100(16):9452-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  126. Comparison of inflammatory elements in nasal lavage and induced sputum following occupational exposure to moldy-building microbes.
    Authors: Purokivi M, Hirvonen MR, Roponen M, Randell J, Vahteristo M, Tukiainen H
    Inhal Toxicol, 2002;14(6):653-62.
    Species: Human
    Sample Type: Sputum
  127. Inflammatory mediators in nasal lavage, induced sputum and serum of employees with rheumatic and respiratory disorders.
    Authors: Roponen M, Kiviranta J, Seuri M, Tukiainen H, Myllykangas-Luosujarvi R, Hirvonen MR
    Eur. Respir. J., 2001;18(3):542-8.
    Species: Human
    Sample Type: Serum
  128. Reproducibility of measurements of exhaled NO, and cell count and cytokine concentrations in induced sputum.
    Authors: Purokivi M, Randell J, Hirvonen MR, Tukiainen H
    Eur. Respir. J., 2000;16(2):242-6.
    Species: Human
    Sample Type: Sputum
  129. Effects of a short-course of amoxicillin/clavulanic acid on systemic and mucosal immunity in healthy adult humans.
    Authors: Dufour V, Millon L, Faucher JF, Bard E, Robinet E, Piarroux R, Vuitton DA, Meillet D
    Int. Immunopharmacol., 205;5(5):917-28.
    Species: Human
    Sample Type: Feces
  130. Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1.
    Authors: Wang R, Jaw JJ, Jaw J, Stutzman N, Stutzman NC, Zou Z, Sun PD
    J Leukoc Biol, 0;91(2):299-309.
    Species: Human
    Sample Type: Cell Culture Supernates
  131. Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
    Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
    Mucosal Immunol, 0;5(2):140-9.
    Species: Human
    Sample Type: Cell Culture Supernates
Expand to show all 131 Citations
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 Human TNF-alpha DuoSet ELISA DY210
: Human TNF-alpha DuoSet ELISA [DY210]

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Sample TestedU937 human histiocytic lymphoma cell line
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