TNF-alpha (Tumor necrosis factor alpha) plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha was first identified as a cytotoxic factor produced by macrophages capable of killing mouse tumor cells. It is the prototypic ligand and along with Lymphotoxin-alpha, were identified as the first members of the TNF superfamily. Active TNF-alpha and other members of the TNF superfamily exist as a homotrimer with high structural homology. Receptor binding occurs at the interface of two TNF-alpha monomers. And receptor activation occurs when all three monomer interfaces are engaged with a receptor. For TNF-alpha, receptor binding and activation occurs through TNF R1 or TNF RII, and subsequently leads to activation of NF-kB or MAPK signaling pathways. Another pathway that TNF-alpha can activate utilizes the death domain of TNF RI to induce apoptosis. TNF-alpha promotes the inflammatory response largely through NF-kB signaling, and inhibition of TNF-alpha has proven successful in treating many autoimmune disorders. TNF-alpha is also present on the cell surface as membrane-bound TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells. TNF-alpha protein is translated as a type II transmembrane protein containing an N-terminal transmembrane domain. The soluble cytokine is released from its cell-anchoring TM domain by proteolytic processing by metalloproteases.
Human TNF-alpha Magnetic Luminex® Performance Assay
R&D Systems | Catalog # LUHM210
Luminex High Performance Assays
Human TNF-alpha Magnetic Luminex® Performance Assay is specifically designed for multiplex detection of human TNF-alpha with a panel of related analytes in serum, plasma, or tissue culture supernatants. Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure the best performance of your multiplex assay.
This assay is customizable, select some or all the analytes in the entire panel. Customize your panel with the Luminex Assay Customization Tool.
Key Product Details
Panel
Human Obesity Panel
Assay Type
Format
Species
Analytes Detected
Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.
Build Your Assay
Product Features
Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile Human TNF-alpha with a panel of related analytes in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Quick turn-around time: Kits are typically shipped within 2-3 days of ordering*
*Please allow additional time for international orders.
Performance Validation
R&D Systems Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.
Base Kit Contents
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
Bead Set Contents
- User-mixed antibody-coated Magnetic beads in individual vials
- User-mixed cocktail of biotinylated detection antibody
Precision
|
Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Three samples of known concentration were tested in twenty-five separate assays to assess precision between assays. |
||||||
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 25 | 25 | 25 |
| Mean (pg/mL) | 39.2 | 232 | 1326 | 44 | 234 | 1237 |
| Standard deviation | 1.66 | 8.56 | 63.9 | 3.0 | 15 | 87 |
| CV (%) | 4.2 | 3.7 | 4.8 | 7.3 | 6.2 | 7.0 |
Recovery
|
Samples containing and/or spiked with human TNF-a were evaluated for recovery. |
||||||
| Sample Type | Average % Recovery | Range (%) | ||||
|---|---|---|---|---|---|---|
| Cell culture supernate | 93 | 80-108 | ||||
| Serum | 108 | 97-114 | ||||
| EDTA plasma | 99 | 78-128 | ||||
| Heparin plasma | 105 | 95-115 | ||||
Linearity
|
Samples containing and/or spiked with human TNF-a were serially diluted to evaluate assay linearity. |
|||||||||
| Cell culture supernate | Serum | EDTA plasma | Heparin plasma | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 1:2 | Average % of Expected | 108 | 104 | 107 | 101 | ||||
| Range (%) | 96-117 | 96-116 | 95-122 | 91-108 | |||||
| 1:4 | Average % of Expected | 108 | 107 | 110 | 98 | ||||
| Range (%) | 90-121 | 96-123 | 90-135 | 94-105 | |||||
| 1:8 | Average % of Expected | 108 | 110 | 111 | 96 | ||||
| Range (%) | 87-120 | 87-120 | 67-145 | 96-98 | |||||
Which Luminex Assay is Right for Me?
Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays.
High Performance Assays are optimized and validated as complete panels with a focused set of analytes. You can choose from Fixed Panels, Premixed options, or Configurable User Mix formats depending on your needs.
Discovery Assays deliver maximum flexibility, enabling the creation of fully customized panels tailored to your research needs. With universal reagents validated for compatibility across all analytes in the menu, they support endless customization possibilities.
Additional Information About Luminex Assays
All R&D Systems Luminex Assays are designed for use with any Luminex instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.
Luminex assays use color-coded, magnetic microparticles coated with analyte-specific antibodies to enable multiplex quantitation. During the assay, standards and samples are added to the wells, where target analytes bind to the capture antibodies. A biotinylated detection antibody cocktail is then introduced, followed by a Streptavidin-PE conjugate that generates a fluorescent signal proportional to the amount of analyte present.
Each microparticle is identified by a unique signature and the signal intensity is measured using a Luminex instrument. These assays are designed to deliver reliable and reproducible results with validated antibody pairs and optimized protocols. They are well suited for applications that require sensitivity, accuracy, and confidence in your data while enabling efficient multiplexing to measure multiple targets simultaneously.
Scientific Data Images for Human TNF-alpha Magnetic Luminex® Performance Assay
Quantist software graph of signal intensity versus concentration for TNF-alpha in the Human Cytokine A Luminex Panel
Preparation and Storage
Shipping
Stability & Storage
Background: TNF-alpha
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional TNF-alpha Products
Product Documents for Human TNF-alpha Magnetic Luminex® Performance Assay
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human TNF-alpha Magnetic Luminex® Performance Assay
Luminex® is a registered trademark of Luminex Corporation.
For research use only
Related Research Areas
Citations for Human TNF-alpha Magnetic Luminex® Performance Assay
Powered by Bioz
Customer Reviews for Human TNF-alpha Magnetic Luminex® Performance Assay (2)
Have you used Human TNF-alpha Magnetic Luminex® Performance Assay?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
-
Sample Tested: SalivaVerified Customer | Posted 02/08/2019
-
Sample Tested: SalivaVerified Customer | Posted 01/29/2019
There are no reviews that match your criteria.
Associated Pathways
Apoptosis Signaling Pathway
Dendritic Cell Lineage Development Pathways
Hematopoietic Stem Cell Differentiation Pathways & Lineage-specific Markers
IL-2 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
Innate Lymphoid Cell Differentiation Pathways
mTOR Signaling Pathway
NOD-like Receptor Signaling Pathways
Th1 Differentiation Pathway
TNF Superfamily Pathway: Human Ligand-Receptor Interactions & their Associated Functions
Toll-Like Receptor Signaling Pathways
