Human TRAIL R3/TNFRSF10C Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human TRAIL R3/TNFRSF10C in direct ELISAs and Western blots. In direct ELISAs, approximately 20%‑40% cross-reactivity with recombinant human (rh) TRAIL R1 is observed and no cross-reactivity with rhTRAIL R2 or rhTRAIL R4 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 90903
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TRAIL R3/TNFRSF10C
    Ala26-Ala221
    Accession # O14798
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize TRAIL R3/TNFRSF10C-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.02-0.08 µg/mL in the presence of 10 ng/mL Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera, 20 ng/mL of Recombinant Human TRAIL/TNFSF10, and 1 µg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
TRAIL R3/TNFRSF10C Inhibition of
TRAIL/TNFSF10-induced Cytotoxicity and
Neutralization by Human
TRAIL R3/TNFRSF10C Antibody.
In the presence of the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera (Catalog # 630-TR) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TEC) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (20 ng/mL) activity elicited by Recombinant Human
TRAIL R3/TNFRSF10C
Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concentrations of Human TRAIL R3/TNFRSF10C Monoclonal Antibody (Catalog # MAB630). The ND50 is typically 0.02-0.08 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL R3/TNFRSF10C

TRAIL R3, also called TNFRSF10C, DcR1 (decoy receptor 1), LIT, and TRID, is a glycosylphosphatidylinositol-linked membrane protein which binds TRAIL (Apo2 Ligand) with high affinity (1, 2). TRAIL R3 contains a TRAIL-binding extracellular cysteine-rich domain but lacks an intracellular signaling domain. As a result, binding of TRAIL to TRAIL R3 does not trigger a pro-apoptotic signal. TRAIL R3 has been shown to protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-induced apoptosis.

  • References:
    1. Sheridan, J.P. et al. (1997) Science 277:818.
    2. Golstein, P. (1997) Curr. Biol. 7:R750.
  • Long Name:
    TRAIL Receptor 3
  • Entrez Gene IDs:
    8794 (Human)
  • Alternate Names:
    Antagonist decoy receptor for TRAIL/Apo-2L; CD263 antigen; CD263; cytotoxic TRAIL receptor-3; DcR1; DCR1MGC149502; Decoy receptor 1; Decoy TRAIL receptor without death domain; LITMGC149501; Lymphocyte inhibitor of TRAIL; TNFRSF10C; TRAIL R3; TRAIL receptor 3; TRAIL receptor without an intracellular domain; TRAILR3; TRAIL-R3; TRAILR3TNF-related apoptosis-inducing ligand receptor 3; TRIDDCR1-TNFR; tumor necrosis factor receptor superfamily member 10C; tumor necrosis factor receptor superfamily, member 10c, decoy without anintracellular domain
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