Human TROP-2 Antibody

(9 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) MCAM, rhNCAM‑L1, and rhBCAM is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TROP-2
    Thr88-Thr274
    Accession # P09758
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TROP‑2 by Western Blot. Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TROP‑2 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of TROP‑2 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Immunohistochemistry
TROP‑2 in Human Brain. TROP‑2 was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TROP-2

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

  • References:
    1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
    2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
    3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
    4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
    5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.
  • Long Name:
    Tumor-associated Calcium Signal Transducer 2
  • Entrez Gene IDs:
    4070 (Human); 56753 (Mouse)
  • Alternate Names:
    Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. Trop2 enhances invasion of thyroid cancer by inducing MMP2 through ERK and JNK pathways
    Authors: H Guan, Z Guo, W Liang, H Li, G Wei, L Xu, H Xiao, Y Li
    BMC Cancer, 2017;17(1):486.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  2. First-in-Human Trial of a Novel Anti-Trop-2 Antibody-SN-38 Conjugate, Sacituzumab Govitecan, for the Treatment of Diverse Metastatic Solid Tumors.
    Authors: Starodub A, Ocean A, Shah M, Guarino M, Picozzi V, Vahdat L, Thomas S, Govindan S, Maliakal P, Wegener W, Hamburger S, Sharkey R, Goldenberg D
    Clin Cancer Res, 2015;21(17):3870-8.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  3. Trop-2 is a determinant of breast cancer survival.
    Authors: Ambrogi, Federico, Fornili, Marco, Boracchi, Patrizia, Trerotola, Marco, Relli, Valeria, Simeone, Pasquale, La Sorda, Rossana, Lattanzio, Rossano, Querzoli, Patrizia, Pedriali, Massimo, Piantelli, Mauro, Biganzoli, Elia, Alberti, Saverio
    PLoS ONE, 2014;9(5):e96993.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Expression of tumor-associated calcium signal transducer 2 in patients with salivary adenoid cystic carcinoma: Correlation with clinicopathological features and prognosis.
    Authors: Xia Y, Li B, Gao N, Xia H, Men Y, Liu Y, Liu Z, Chen Q, Li L
    Oncol Lett, 2014;8(4):1670-1674.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Upregulation of Trop-2 quantitatively stimulates human cancer growth.
    Authors: Trerotola, M, Cantanelli, P, Guerra, E, Tripaldi, R, Aloisi, A L, Bonasera, V, Lattanzio, R, de Lange, R, Weidle, U H, Piantelli, M, Alberti, S
    Oncogene, 2013;32(2):222-33.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. Uterine and ovarian carcinosarcomas overexpressing Trop-2 are sensitive to hRS7, a humanized anti-Trop-2 antibody.
    Authors: Raji R, Guzzo F, Carrara L
    J. Exp. Clin. Cancer Res., 2011;30(0):106.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-beta1-dependent human epidermal Langerhans cells.
    Authors: Eisenwort G, Jurkin J, Yasmin N, Bauer T, Gesslbauer B, Strobl H
    J. Invest. Dermatol., 2011;131(10):2049-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy.
    Authors: Nakatsukasa M, Kawasaki S, Yamasaki K
    Am. J. Pathol., 2010;177(3):1344-55.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  9. Identification of Trop-2 as an oncogene and an attractive therapeutic target in colon cancers.
    Authors: Wang J, Day R, Dong Y, Weintraub SJ, Michel L
    Mol. Cancer Ther., 2008;7(2):280-5.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
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