Human TSLPR Antibody

Catalog # Availability Size / Price Qty
Detection of TSLP R in Human PBMC Monocytes by Flow Cytometry.
2 Images
Product Details
Citations (4)
Supplemental Products

Human TSLPR Antibody Summary

Species Reactivity
Detects human TSLP R in direct ELISAs and Western blots. Does not cross‑react with recombinant mouse TSLP R.
Monoclonal Mouse IgG1 Clone # 147036
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human TSLP R
Accession # Q9HC73
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human TSLP R Fc Chimera (Catalog # 981-TR)
Flow Cytometry
0.25 µg/106 cells
Human PBMC monocytes and NS0/hTSLPR
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TSLP R antibody in Human PBMC Monocytes antibody by Flow Cytometry. View Larger

Detection of TSLP R in Human PBMC Monocytes by Flow Cytometry. Human PBMC monocytes were stained with Human TSLP R Monoclonal Antibody (Catalog # MAB981) followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B) and Human CD14 Fluorescein-conjugated Monoclonal Antibody (FAB3832F). Quadrant markers were set based on control antibody staining (MAB002).

Flow Cytometry View Larger

Detection of TSLPR in NS0/hTSLPR cells by Flow Cytometry. NS0/hTSLPR cells were stained with Mouse Anti-Human TSLPR Monoclonal Antibody (Catalog # MAB981, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSLPR

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS-like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3‑6). The TSLP R and IL-7 R alpha are co‑expressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (5).

  1. Fujio, K. et al. (2000) Blood 95:2204.
  2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
  3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  4. Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
  5. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  6. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
Long Name
Thymic Stromal Lymphopoietin Receptor
Entrez Gene IDs
64109 (Human); 57914 (Mouse); 171499 (Rat)
Alternate Names
CRL2; CRL2cytokine receptor CRL2 precusor; CRLF2; CRLF2Y; CRLM-2; Cytokine receptor-like 2; cytokine receptor-like factor 2; ILXR; IL-XR; P2RY8/CRLF2 fusion; PCOR1; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP R; TSLP receptor; TSLPR

Product Datasheets

You must select a language.


Citations for Human TSLPR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Thymic stromal chemokine TSLP acts through Th2 cytokine production to induce cutaneous T cell lymphoma
    Cancer Res, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. DeltaNp63 controls a TLR3-mediated mechanism that abundantly provides thymic stromal lymphopoietin in atopic dermatitis.
    Authors: Kubo T, Kamekura R, Kumagai A, Kawata K, Yamashita K, Mitsuhashi Y, Kojima T, Sugimoto K, Yoneta A, Sumikawa Y, Yamashita T, Sato N, Himi T, Ichimiya S
    PLoS ONE, 2014;9(8):e105498.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Thymic stromal lymphopoietin-induced human asthmatic airway epithelial cell proliferation through an IL-13-dependent pathway.
    Authors: Semlali A, Jacques E, Koussih L, Gounni AS, Chakir J
    J. Allergy Clin. Immunol., 2010;125(4):844-50.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Thymic stromal lymphopoietin receptor-mediated IL-6 and CC/CXC chemokines expression in human airway smooth muscle cells: role of MAPKs (ERK1/2, p38, and JNK) and STAT3 pathways.
    Authors: Shan L, Redhu NS, Saleh A
    J. Immunol., 2010;184(12):7134-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human TSLPR Antibody

There are currently no reviews for this product. Be the first to review Human TSLPR Antibody and earn rewards!

Have you used Human TSLPR Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review