Human TSPAN8 Antibody

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Human TSPAN8 Antibody in Data
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Product Details
Citations (1)
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Reviews (1)

Human TSPAN8 Antibody Summary

Species Reactivity
Detects human TSPAN8 in Western blots.
Monoclonal Rat IgG2B Clone # 458811
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human TSPAN8
Met1-Lys237 (Ile35Val)
Accession # AAH05246
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human TSPAN8 antibody by Western Blot. View Larger

Detection of Human TSPAN8 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TSPAN8 at approximately 35 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of TSPAN8 antibody in HT‑29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TSPAN8 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry TSPAN8 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC). View Larger

TSPAN8 in HT‑29 Human Cell Line. TSPAN8 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN8

Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 superfamily. It is a cell surface 27‑34 kDa glycoprotein with 4 transmembrane segments, and a large extracellular loop (LEL) domain from amino acids (aa) 106‑206. Both the N- and C-termini are intracellular. TSPAN8 is expressed by multiple tumor types as well as smooth and skeletal muscle cells, endothelial cells, hematopoietic progenitor cells and non-keratinized squamous epithelium. As with other TSPAN family molecules, TSPAN8 acts as an organizer of microdomains in membranes. Molecules that may be found in these domains include CD151 (another TSPAN family member), beta 1 Integrins, Integrin alpha 4 beta 6, EpCAM, and CD13. TSPAN8 would appear to remove Integrins from the cell surface, facilitating cell motility which, in the case of tumor cells, results in metastasis. TSPAN8 shares 53% aa sequence identity with both mouse and rat TSPAN8.

Long Name
Tetraspanin 8/Transmembrane 4 Superfamily, Member 3
Entrez Gene IDs
7103 (Human); 216350 (Mouse); 171048 (Rat)
Alternate Names
CO-029; tetraspanin 8; TM4SF3; TM4SF3tspan-8; Transmembrane 4 superfamily member 3tetraspanin-8; TSPAN8; Tspan-8; Tumor-associated antigen CO-029

Product Datasheets

Citation for Human TSPAN8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers
    Authors: K Dissanayak, M Nõmm, F Lättekivi, Y Ressaissi, K Godakumara, A Lavrits, G Midekessa, J Viil, R Bæk, MM Jørgensen, S Bhattachar, A Andronowsk, A Salumets, Ü Jaakma, A Fazeli
    Theriogenology, 2020;149(0):104-116.
    Species: Mouse
    Sample Types:
    Applications: Microarray


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Cell and Tissue Staining Kits

Isotype Controls

Rat IgG2B Isotype Control

Reconstitution Buffers

Secondary Antibodies

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Human TSPAN8 Antibody
By Anonymous on 03/23/2018
Application: WB Sample Tested: Purified protein Species: Human