Human/Mouse TSC2 Antibody

R&D Systems | Catalog # MAB40401

R&D Systems
Discontinued Product
MAB40401 has been discontinued. View all TSC2 products.

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 614204
View all TSC2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TSC2
Val550-Leu850
Accession # P49815

Specificity

Detects human and mouse TSC2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse TSC2 Antibody

Detection of Human and Mouse TSC2 antibody by Western Blot.

Detection of Human and Mouse TSC2 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse TSC2 Monoclonal Antibody (Catalog # MAB40401) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for TSC2 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TSC2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

TSC2 in HeLa Human Cell Line.

TSC2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse TSC2 Monoclonal Antibody (Catalog # MAB40401) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TSC2 antibody in Human Kidney by Immunohistochemistry (IHC-P).

TSC2 in Human Kidney.

TSC2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse TSC2 Monoclonal Antibody (Catalog # MAB40401) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Shows Human TSC2 Specificity Using Knockout Cell Line.

Western blot shows lysates of HAP1 human near-haploid cell line and TSC2 knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse TSC2 Monoclonal Antibody (Catalog # MAB40401) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for TSC2 at approximately 190 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Applications for Human/Mouse TSC2 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Knockout Validated

TSC2 is specifically detected in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line.

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB40401 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSC2

TSC2, also called tuberin, complexes with TSC1 (hamartin) to inhibit cell proliferation by antagonizing mTOR/p70S6K signaling. Its location either in the cytoplasm or the nucleus is regulated by phosphorylation and the cell cycle. Numerous point mutations are associated with development of tumors (hamartomas) in kidney, heart, skin and brain. Human TSC2 shares 91% amino acid (aa) identity with mouse TSC2 over aa 550-850.  Splice variants that are missing aa 946 to 988 or 989 may show different tissue distribution than full length TSC2.

Long Name

Tuberous Sclerosis 2

Alternate Names

LAM, TSC4, Tuberin

Entrez Gene IDs

7249 (Human); 22084 (Mouse); 24855 (Rat)

Gene Symbol

TSC2

UniProt

P49815

Additional TSC2 Products

Product Documents for Human/Mouse TSC2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse TSC2 Antibody

For research use only

Related Research Areas

Interferons

Citations for Human/Mouse TSC2 Antibody

Customer Reviews for Human/Mouse TSC2 Antibody (1)

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  • Human/Mouse TSC2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 03/07/2022
    Human/Mouse TSC2 Antibody MAB40401

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Protocols

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