Detection of Human Tyrosylprotein Sulfotransferase 2/TPST2 by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Tyrosylprotein Sulfotransferase 2/TPST2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6236) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tyrosylprotein Sulfotransferase 2/TPST2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human Tyrosylprotein Sulfotransferase 2 (TPST2) is a 42
kDa (predicted) member of a sulfotransferase family. Two human tyrosylprotein
sulfotransferases (TPSTs), TPST1 and TPST2 mediate tyrosine O-sulfation of more
than 60 different proteins in the Golgi, transferring sulfate from
3'-phosphoadenosine 5'‑phosphosulfate to tyrosine residues contained in
polypeptides with acidic motifs to form a tyrosine O-sulfate ester. TSPT1 and TSPT2
are widely expressed, they show similar tissue distribution and overlapping
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