Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Tyrosylprotein Sulfotransferase 2/TPST2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recominant human Tyrosylprotein Sulfotransferase 2/TPST2 
Gln26-Ser377 (predicted)
Accession # O60704

Specificity

Detects human Tyrosylprotein Sulfotransferase 2/TPST2 in direct ELISAs and Western blots. In direct ELISAs, less than 7% cross-reactivity with recombinant human TPST1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Tyrosylprotein Sulfotransferase 2/TPST2 Antibody

Detection of Human Tyrosylprotein Sulfotransferase 2/TPST2 antibody by Western Blot.

Detection of Human Tyrosylprotein Sulfotransferase 2/TPST2 by Western Blot.

Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Tyrosylprotein Sulfotransferase 2/TPST2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6236) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tyrosylprotein Sulfotransferase 2/TPST2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Tyrosylprotein Sulfotransferase 2/TPST2 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Human pancreas tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tyrosylprotein Sulfotransferase 2/TPST2

Human Tyrosylprotein Sulfotransferase 2 (TPST2) is a 42 kDa (predicted) member of a sulfotransferase family. Two human tyrosylprotein sulfotransferases (TPSTs), TPST1 and TPST2 mediate tyrosine O-sulfation of more than 60 different proteins in the Golgi, transferring sulfate from 3'-phosphoadenosine 5'‑phosphosulfate to tyrosine residues contained in polypeptides with acidic motifs to form a tyrosine O-sulfate ester. TSPT1 and TSPT2 are widely expressed, they show similar tissue distribution and overlapping substrate specificity.

Alternate Names

Tyrosylprotein Sulfotransferase 2

Entrez Gene IDs

8459 (Human)

Gene Symbol

TPST2

UniProt

O60704

Additional Tyrosylprotein Sulfotransferase 2/TPST2 Products

Product Documents for Human Tyrosylprotein Sulfotransferase 2/TPST2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Tyrosylprotein Sulfotransferase 2/TPST2 Antibody

For research use only

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