Human uPAR Antibody

Catalog # Availability Size / Price Qty
MAB807-SP
MAB807-100
MAB807-500
Detection of uPAR in Human Blood Granulocytes by Flow Cytometry.
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Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (2)

Human uPAR Antibody Summary

Species Reactivity
Human
Specificity
Detects human uPAR in direct ELISAs and Western blots. When used in a sandwich ELISA in combination with the biotinylated anti-human uPAR detection antibody (Catalog # BAF807), no significant cross-reactivity was observed with recombinant mouse uPAR.
Source
Monoclonal Mouse IgG1 Clone # 62022
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human uPAR
Leu23-Arg303
Accession # Q03405
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human uPAR (Catalog # 807-UK)
under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.5-1.5 µg/mL of this antibody will block 50% of the binding of 30 ng/mL of Recombinant Human uPAR (Catalog # 807-UK) to immobilized Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) coated at 500 ng/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human uPAR Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human uPAR Biotinylated Antibody (Catalog # BAF807)

Standard: Recombinant Human uPAR Protein (Catalog # 807-UK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of uPAR antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of uPAR in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: uPAR

The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a
single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a
C‑terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific.

References
  1. Dear, A.E. and R.L. Medcalf (1988) Eur. J. Biochemistry 252:185.
Long Name
Urokinase-type Plasminogen Activator Receptor
Entrez Gene IDs
5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)
Alternate Names
CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3

Product Datasheets

Citations for Human uPAR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. E4 engages uPAR and enolase-1 and activates urokinase to exert antifibrotic effects
    Authors: S Sharma, T Watanabe, T Nishimoto, T Takihara, L Mlakar, XX Nguyen, M Sanderson, Y Su, RA Chambers, C Feghali-Bo
    JCI Insight, 2021;6(24):.
    Species: Human
    Sample Types: Cell Fractions
    Applications: Immunoprecipitation
  2. Negative regulation of urokinase receptor activity by a GPI-specific phospholipase C in breast cancer cells
    Authors: M van Veen, E Matas-Rico, K van de Wet, D Leyton-Pui, KM Kedziora, V De Lorenzi, Y Stijf-Bult, B van den Br, K Jalink, N Sidenius, A Perrakis, WH Moolenaar
    Elife, 2017;6(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The periodontal pathogen Porphyromonas gingivalis preferentially interacts with oral epithelial cells in S phase of the cell cycle
    Authors: FB Al-Taweel, CW Douglas, SA Whawell
    Infect Immun, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Soluble Urokinase Receptor Is Released Selectively by Glioblastoma Cells That Express Epidermal Growth Factor Receptor Variant III and Promotes Tumor Cell Migration and Invasion.
    Authors: Gilder A, Jones K, Hu J, Wang L, Chen C, Carter B, Gonias S
    J Biol Chem, 2015;290(24):14798-809.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Intracellular modifiers of integrin alpha 6p production in aggressive prostate and breast cancer cell lines.
    Authors: Kacsinta A, Rubenstein C, Sroka I, Pawar S, Gard J, Nagle R, Cress A
    Biochem Biophys Res Commun, 2014;454(2):335-40.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Urinary soluble urokinase receptor levels are elevated and pathogenic in patients with primary focal segmental glomerulosclerosis.
    Authors: Huang J, Liu G, Zhang Y, Cui Z, Wang F, Liu X, Chu R, Zhao M
    BMC Med, 2014;12(0):81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Plasmin cleavage of von Willebrand factor as an emergency bypass for ADAMTS13 deficiency in thrombotic microangiopathy.
    Authors: Tersteeg C, de Maat S, De Meyer S, Smeets M, Barendrecht A, Roest M, Pasterkamp G, Fijnheer R, Vanhoorelbeke K, de Groot P, Maas C
    Circulation, 2014;129(12):1320-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  8. Activation of the plasma kallikrein-kinin system on human lung epithelial cells.
    Authors: Vergiliana JF, Asokananthan N, Stewart GA
    Biol. Chem., 2010;391(9):1067-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. AP-1-controlled hepatocyte growth factor activation promotes keratinocyte migration via CEACAM1 and urokinase plasminogen activator/urokinase plasminogen receptor.
    Authors: Schnickmann S, Camacho-Trullio D, Bissinger M, Eils R, Angel P, Schirmacher P, Szabowski A, Breuhahn K
    J. Invest. Dermatol., 2009;129(5):1140-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  11. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  12. Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma.
    Authors: Brooks AM, Bates ME, Vrtis RF, Jarjour NN, Bertics PJ, Sedgwick JB
    Am. J. Respir. Cell Mol. Biol., 2006;35(4):503-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. LDL-receptor-related protein regulates beta2-integrin-mediated leukocyte adhesion.
    Authors: Spijkers PP, da Costa Martins P, Westein E, Gahmberg CG, Zwaginga JJ, Lenting PJ
    Blood, 2005;105(1):170-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human uPAR Antibody
By Anonymous on 09/24/2021
Application: WB Sample Tested: Serum Species: Human

The impact of prolactin treatments was used to assess human uPAR expression using western blot. Results were reliable, the intra-assay variation was low, and the obtained outcomes were in agreement with reported results from the literature. In the picture, the densitometric analysis from western blot is depicted, showing a control (without prolactin treatment), a pharmacological treatment using PRL treatments, a specific 16 kDa PRL treatment, and methotrexate as PRLR inhibitor.


Human uPAR Antibody
By Anonymous on 09/04/2021
Application: WB Sample Tested: Human Blood Granulocytes Species: Human