Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Antibody Array Development, ELISA Microarray Development, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 62022
View all uPAR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human uPAR
Leu23-Arg303
Accession # Q03405

Specificity

Detects human uPAR in direct ELISAs and Western blots. When used in a sandwich ELISA in combination with the biotinylated anti-human uPAR detection antibody (Catalog # BAF807), no significant cross-reactivity was observed with recombinant mouse uPAR.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human uPAR Antibody

Detection of uPAR antibody in Human Blood Granulocytes antibody by Flow Cytometry.

Detection of uPAR in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Detection of uPAR by Western Blot

Detection of uPAR by Western Blot

Localization of CTSB and members of the proteolytic cascade in the caveolae. Representative WB showing expression of (A) CTSB (pro-CTSB, sc-CTSB, dc-CTSB) and CAV1, or (B) ANXA2, pro-uPA and uPAR in caveolae-enriched fractions (2–5, 15 μL) and cell lysates (CL) from hTM cells. l.e.: lower exposure; h.e.: higher exposure. (C) Representative WB showing expression of pro-CTSB, pro-uPA, and uPA in conditioned media (15 μL) from hTM cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33379277), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of uPAR by Western Blot

Detection of uPAR by Western Blot

Immunoblot image are shown (A), along with densitometric analysis of (B) PDGFR beta, (C) Cyr61, (D) CD97, (E) Glypican-1, (F) MUC18/CD146 and (G) uPAR. (H) A summary of proteins detected by mass spectrometry and immunoblot. Graphs represent mean ± SEM of n = 3 independent biological experiments, shown as 1,2,3. Unpaired t-test was used as statistical test. *P < 0.05, **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36171564), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of uPAR by Western Blot

Detection of uPAR by Western Blot

Immunoblot image are shown (A), along with densitometric analysis of (B) PDGFR beta, (C) Cyr61, (D) CD97, (E) Glypican-1, (F) MUC18/CD146 and (G) uPAR. (H) A summary of proteins detected by mass spectrometry and immunoblot. Graphs represent mean ± SEM of n = 3 independent biological experiments, shown as 1,2,3. Unpaired t-test was used as statistical test. *P < 0.05, **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36171564), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human uPAR Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.5-1.5 µg/mL of this antibody will block 50% of the binding of 30 ng/mL of Recombinant Human uPAR (Catalog # 807-UK) to immobilized Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) coated at 500 ng/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood granulocytes

Western Blot

1 µg/mL
Sample: Recombinant Human uPAR (Catalog # 807-UK)
under non-reducing conditions only

Human uPAR Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human uPAR Biotinylated Antibody (Catalog # BAF807)
  • Standard: Recombinant Human uPAR Protein (Catalog # 807-UK)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 5 using MAB807 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: uPAR

The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a
single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a
C‑terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific.

References

  1. Dear, A.E. and R.L. Medcalf (1988) Eur. J. Biochemistry 252:185.

Long Name

Urokinase-type Plasminogen Activator Receptor

Alternate Names

PLAUR

Entrez Gene IDs

5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)

Gene Symbol

PLAUR

UniProt

Q03405

Additional uPAR Products

Product Documents for Human uPAR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human uPAR Antibody

For research use only

Citations for Human uPAR Antibody

Customer Reviews for Human uPAR Antibody (3)

5 out of 5
3 Customer Ratings
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human uPAR Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 05/27/2022
    Human uPAR Antibody MAB807
  • Human uPAR Antibody
    Name: Martin Torres
    Application: Western Blot
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 09/24/2021
    The impact of prolactin treatments was used to assess human uPAR expression using western blot. Results were reliable, the intra-assay variation was low, and the obtained outcomes were in agreement with reported results from the literature. In the picture, the densitometric analysis from western blot is depicted, showing a control (without prolactin treatment), a pharmacological treatment using PRL treatments, a specific 16 kDa PRL treatment, and methotrexate as PRLR inhibitor.
    Human uPAR Antibody MAB807
  • Human uPAR Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human Blood Granulocytes
    Species: Human
    Verified Customer | Posted 09/04/2021
    Human uPAR Antibody MAB807

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