Detects human VAMP-5 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) VAMP-7 is observed, and less than 1% cross-reactivity with rhVAMP-1, rhVAMP-2, and rhVAMP-8 is observed.
Polyclonal Sheep IgG
E. coli-derived recombinant human VAMP-5 Ala2-Cys72 Accession # O95183
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
VAMP-5 in Human Skeletal Muscle.
VAMP-5 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Sheep Anti-Human VAMP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7366) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
VAMP-5 (vesicle associated membrane protein 5; also myobrevin) is a member of the synaptobrevin family of proteins. Although its predicted MW is 11 kDa, it runs anomalously at 16 kDa in SDS-Page. VAMP-5 is a type IV transmembrane (TM) protein (i.e.-a type II TM protein whose C-terminus is almost completely transmembrane) that is found in both trans-Golgi and plasma membranes of myotubes (mature skeletal and cardiac muscle cells). While VAMPs are commonly associated with vesicle fusion to t-SNARE in cell membranes, VAMP-5 appears to show no such activity. Human VAMP-5 is 116 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-72) with one vSNARE coiled-coil homology domain, a 21 aa TM segment, and 23 aa C-terminal luminal domain. Over aa 1-72, human VAMP-5 shares 75% aa identity with mouse VAMP-5.
Vesicle-Associated Membrane Protein 5
Entrez Gene IDs:
10791 (Human); 53620 (Mouse); 89818 (Rat)
Myobrevin; VAMP5; VAMP-5; vesicle-associated membrane protein 5 (myobrevin); vesicle-associated membrane protein 5
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