Human VCAM-1/CD106 Antibody

Catalog #: BBA19
7 Citations Datasheet / COA / SDS

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BBA19

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Detection of Human VCAM‑1/CD106 by Simple WesternTM.

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Product Details

Citations (7)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VCAM-1/CD106 Antibody Summary

Species Reactivity

Human

Specificity

Detects VCAM‑1/CD106 in Western blots. VCAM‑1/CD106 has been screened using CHO cells transfected with cDNAs for ICAM-1 and VCAM-1.

Source

Polyclonal Goat Serum

Purification

N/A

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human VCAM-1

Formulation

Lyophilized from a 0.2 μm filtered solution in Serum.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

Recombinant Human VCAM‑1/CD106 (Catalog # 809-VR)

Simple Western

20 µg/mL

HUVEC human umbilical vein endothelial cells

Immunohistochemistry

1:200 dilution

Immersion fixed paraffin-embedded sections of human kidney subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Simple Western

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Detection of Human VCAM‑1/CD106 by Simple Western^TM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 10 ng/ml Recombinant Human TNF‑ alpha (210-TA) for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for VCAM‑1/CD106 at approximately 132 kDa (as indicated) using 20 µg/mL of Goat Anti-Human VCAM‑1/CD106 Polyclonal Antibody (Catalog # BBA19). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute in 0.5 mL of sterile water.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

Vascular cell adhesion molecule (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.

Long Name

Vascular Cell Adhesion Molecule 1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Alternate Names

CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human VCAM-1/CD106 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Perivascular Adventitial Fibroblast Specialization Accompanies T Cell Retention in the Inflamed Human Dermis
Authors: AMS Barron, JC Mantero, JD Ho, B Nazari, KL Horback, J Bhawan, R Lafyatis, C Lam, JL Browning
J. Immunol., 2018-12-03;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Plasticity-related gene 5 promotes spine formation in murine hippocampal neurons.
Authors: Coiro P, Stoenica L, Strauss U, Brauer A
J Biol Chem, 2014-07-29;289(36):24956-70.
Outer membrane vesicles derived from Escherichia coli up-regulate expression of endothelial cell adhesion molecules in vitro and in vivo.
Authors: Kim J, Yoon Y, Lee J, Choi E, Yi N, Park K, Park J, Lotvall J, Kim Y, Gho Y
PLoS ONE, 2013-03-14;8(3):e59276.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
Dig. Dis. Sci., 2007-03-30;52(10):2775-83.
Species: Human
Sample Types: Whole Cells
Applications: ICC
TNFalpha increases the inflammatory response to vascular balloon injury without accelerating neointimal formation.
Authors: Miller AM, McPhaden AR, Preston A, Wadsworth RM, Wainwright CL
Atherosclerosis, 2004-12-23;179(1):51-9.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC-P
Characterization of hematopoietic progenitor mobilization in protease-deficient mice.
Authors: Levesque JP, Liu F, Simmons PJ, Betsuyaku T, Senior RM, Pham C, Link DC
Blood, 2004-03-09;104(1):65-72.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot
Functions of domain 1 and 4 of vascular cell adhesion molecule-1 in alpha4 integrin-dependent adhesion under static and flow conditions are differentially regulated.
Authors: Abe Y, Ballantyne CM, Smith CW
J. Immunol., 1996-12-01;157(11):5061-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot