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Human VCAM-1/CD106 Antibody

Catalog #: BBA19
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BBA19

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Detection of Human VCAM‑1/CD106 by Simple WesternTM.
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Citations (12)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VCAM-1/CD106 Antibody Summary

Species Reactivity
Human
Specificity
Detects VCAM‑1/CD106 in Western blots. VCAM‑1/CD106 has been screened using CHO cells transfected with cDNAs for ICAM-1 and VCAM-1.
Source
Polyclonal Goat Serum
Purification
N/A
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human VCAM-1
Formulation
Lyophilized from a 0.2 μm filtered solution in Serum.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
Recombinant Human VCAM‑1/CD106 (Catalog # 809-VR)
Simple Western
20 µg/mL
HUVEC human umbilical vein endothelial cells
Immunohistochemistry
1:200 dilution
Immersion fixed paraffin-embedded sections of human kidney subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Simple Western View Larger

Detection of Human VCAM‑1/CD106 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 10 ng/ml Recombinant Human TNF‑ alpha (210-TA) for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for VCAM‑1/CD106 at approximately 132 kDa (as indicated) using 20 µg/mL of Goat Anti-Human VCAM‑1/CD106 Polyclonal Antibody (Catalog # BBA19). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute in 0.5 mL of sterile water.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

Vascular cell adhesion molecule (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.

Long Name
Vascular Cell Adhesion Molecule 1
Entrez Gene IDs
7412 (Human); 22329 (Mouse)
Alternate Names
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

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Citations for Human VCAM-1/CD106 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Size and targeting to PECAM vs ICAM control endothelial delivery, internalization and protective effect of multimolecular SOD conjugates
    Authors: Vladimir V. Shuvaev, Silvia Muro, Evguenia Arguiri, Makan Khoshnejad, Samira Tliba, Melpo Christofidou-Solomidou et al.
    Journal of Controlled Release
  2. RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border
    Authors: Beatriz Marcos-Ramiro, Diego García-Weber, Susana Barroso, Jorge Feito, María C. Ortega, Eva Cernuda-Morollón et al.
    Journal of Cell Biology
  3. Perivascular Adventitial Fibroblast Specialization Accompanies T Cell Retention in the Inflamed Human Dermis
    Authors: AMS Barron, JC Mantero, JD Ho, B Nazari, KL Horback, J Bhawan, R Lafyatis, C Lam, JL Browning
    J. Immunol., 2018-12-03;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Plasticity-related gene 5 promotes spine formation in murine hippocampal neurons.
    Authors: Coiro P, Stoenica L, Strauss U, Brauer A
    J Biol Chem, 2014-07-29;289(36):24956-70.
  5. Outer membrane vesicles derived from Escherichia coli up-regulate expression of endothelial cell adhesion molecules in vitro and in vivo.
    Authors: Kim J, Yoon Y, Lee J, Choi E, Yi N, Park K, Park J, Lotvall J, Kim Y, Gho Y
    PLoS ONE, 2013-03-14;8(3):e59276.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
    Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
    Dig. Dis. Sci., 2007-03-30;52(10):2775-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. TNFalpha increases the inflammatory response to vascular balloon injury without accelerating neointimal formation.
    Authors: Miller AM, McPhaden AR, Preston A, Wadsworth RM, Wainwright CL
    Atherosclerosis, 2004-12-23;179(1):51-9.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Characterization of hematopoietic progenitor mobilization in protease-deficient mice.
    Authors: Levesque JP, Liu F, Simmons PJ, Betsuyaku T, Senior RM, Pham C, Link DC
    Blood, 2004-03-09;104(1):65-72.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  9. Functions of domain 1 and 4 of vascular cell adhesion molecule-1 in alpha4 integrin-dependent adhesion under static and flow conditions are differentially regulated.
    Authors: Abe Y, Ballantyne CM, Smith CW
    J. Immunol., 1996-12-01;157(11):5061-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Endothelium Activation during Severe Yellow Fever Triggers an Intense Cytokine-Mediated Inflammatory Response in the Liver Parenchyma
    Authors: Alves Olimpio F, Magno FalcAo L, Gaia Carvalho M et al.
    Preprints

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