Human VEGF₁₆₅ Antibody

Detection of VEGF165 by Immunohistochemistry

The intracellular relocation of PHB was induced by vascular endothelial growth factor (VEGF) in CRC (A) Stromal were mixed with LS174T or SW480 or SW620 & were cultured for 24 h. Supernatants were collected & VEGF levels were determined using the enzyme-linked immune sorbent assay (ELISA). *P < 0.01, **P < 0.001. Data are shown as means ± SD. Levels of VEGF expression in the interstitial tissue are shown in primary CRC with metastasis & non-metastasis. *P < 0.001. Data are shown as means ± SEM. (B) Quantitative analysis of wound-healing assays was performed by calculating the percentage of in which PHB was relocated to the direction of wound. *P < 0.01 & **P < 0.001. Data are shown as means ± SD. (C) A schematic model & an experimental example for the polarized migration assay. A mixture of VEGF & Matrigel was placed in area 1, Matrigel alone was placed in area 2, 3, & 4, & the in area 5 were chosen for polarization analysis. in which PHB was located within the 120° angle were counted as being in the direction of VEGF stimulation, & are marked as red stars. The quantitative analysis of polarity assays was performed by calculation of the percentage of in which PHB was relocated to the direction of VEGF stimulation. *P < 0.001 compared with VEGF treatment for 0 h. Data are shown as means ± SD. (D) Co-immunoprecipitation assay with Cdc42. Cdc42 & PHB were expressed in SW480/LS174T with (+) or without (-) VEGF (100 ng/mL) treatment for 24 h. (E) Indicated GST-fusion proteins were incubated with lysates from SW480/LS174T & precipitated with glutathione beads. PHB was detected in the eluates of GST-Cdc42. (F) Co-immunostaining for PHB & Cdc42 in SW480/LS174T with or without VEGF stimulation. The arrowheads indicate PHB & Cdc42 directionality. Scale bars: 10 μm. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29100316), licensed under a CC-BY license. Not internally tested by R&D Systems.