Human VEGF_165b Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB3045U Datasheet / COA / SDS

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FAB3045U-100UG

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Human VEGF_165b Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human VEGF_165b in direct ELISAs and Western blots. Recognizes an epitope within a 9 amino acid sequence at the C-terminus of human VEGF_165b. In direct ELISAs and Western blots, no cross-reactivity with recombinant human

Source

Monoclonal Mouse IgG1 Clone # 56-1

Purification

Protein A or G purified

Immunogen

KLH-conjugated human VEGF_165b synthetic peptide
TCRSLTRKD
Accession # AAL27435

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunofluorescence

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: VEGF

VEGF_165b is the protein resulting from an mRNA splice variant of VEGF. Alternative exon usage results in a 165 amino acid product that is identical to VEGF₁₆₅ except for 6 unique amino acids at the C-terminus. VEGF₁₆₅b inhibits proliferation and migration of endothelial cells and is down-regulated in renal cell carcinomas.

Long Name

Vascular Endothelial Growth Factor

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Alternate Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor