Human VEGFR1/Flt‑1 Biotinylated Antibody Summary
Accession # NP_001153392
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
VEGFR1/Flt‑1 in Human Breast. VEGFR1/Flt-1 was detected in immersion fixed paraffin-embedded sections of human breast array using Human VEGFR1/Flt-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF321) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.
Citations for Human VEGFR1/Flt‑1 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Human Umbilical Tissue-Derived Cells Secrete Soluble VEGFR1 and Inhibit Choroidal Neovascularization
Authors: J Cao, R Yang, TE Smith, S Evans, GW McCollum, SC Pomerantz, T Petley, IR Harris, JS Penn
Mol Ther Methods Clin Dev, 2019;14(0):37-46.
Sample Types: Recombinat Protein
Applications: Western Blot
Characteristics of the specific humoral response in patients with advanced solid tumors after active immunotherapy with a VEGF vaccine, at different antigen doses and using two distinct adjuvants
Authors: J Sánchez Ra, Y Morera Día, M Bequet-Rom, F Hernández-, KH Selman-Hou, A de la Torr, ER Santiesteb, Y Martín Bau, CH Bermúdez B, J de la Torr, JV Gavilondo,, M Ayala Avil
BMC Immunol., 2017;18(1):39.
Sample Types: Serum
Applications: ELISA Development (Detection)
Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease
Sci Rep, 2016;6(0):32382.
Sample Types: Whole Cells
PEDF derived from glial Muller cells: a possible regulator of retinal angiogenesis.
Authors: Eichler W, Yafai Y, Keller T, Wiedemann P, Reichenbach A
Exp. Cell Res., 2004;299(1):68-78.
Sample Types: Cell Lysates
Applications: Western Blot
Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
Eur. J. Clin. Invest., 2003;33(10):883-90.
Sample Types: Plasma
Applications: ELISA Development
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