Human VEGFR1/Flt-1 Antibody

Catalog # Availability Size / Price Qty
AF321
AF321-SP
Human VEGFR1/Flt-1 Antibody in Immunohistochemistry
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Product Details
Citations (29)
FAQs
Supplemental Products
Reviews (2)

Human VEGFR1/Flt-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGFR1/Flt-1 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant mouse VEGFR1 and less than 10% cross-reactivity with recombinant human (rh) VEGFR2 and rhVEGFR3.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR1/Flt-1
Ser27-His687
Accession # NP_001153392
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human VEGFR1/Flt‑1 Fc Chimera (Catalog # 321-FL)
Flow Cytometry
2.5 µg/106 cells
HUVEC human umbilical vein endothelial cells
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 2 ng/mL of Recombinant Human PlGF (Catalog # 264-PG) to immobilized Recombinant Human VEGFR1/Flt-1 Fc Chimera (Catalog # 321-FL) coated at 1 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry VEGFR1/Flt‑1 in Human Breast Cancer Tissue. View Larger

VEGFR1/Flt‑1 in Human Breast Cancer Tissue. VEGFR1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Human VEGFR1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry VEGFR1/Flt‑1 in Human Ovarian Cancer Tissue. View Larger

VEGFR1/Flt‑1 in Human Ovarian Cancer Tissue. VEGFR1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human VEGFR1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR1/Flt-1

Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Long Name
Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs
2321 (Human); 14254 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

Product Datasheets

Citations for Human VEGFR1/Flt-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. MMP14-Containing Exosomes Cleave VEGFR1 and Promote VEGFA-Induced Migration and Proliferation of Vascular Endothelial Cells
    Authors: KY Han, JH Chang, DT Azar
    Invest. Ophthalmol. Vis. Sci., 2019;60(6):2321-2329.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Villous explants from preeclamptic placentas induce sFlt1 in PBMCs: An ex vivo co-culture study
    Authors: SK Gonzales, M Badell, H Cottrell, B Rimawi, V Deepak, N Sidell, A Rajakumar
    Pregnancy Hypertens, 2018;12(0):40-46.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. Intravitreal Injection of AAV Expressing Soluble VEGF Receptor-1 Variant Induces Anti-VEGF Activity and Suppresses Choroidal Neovascularization
    Authors: SHS Lee, HJ Kim, OK Shin, JS Choi, J Kim, YH Cho, J Ha, TK Park, JY Lee, K Park, H Lee
    Invest. Ophthalmol. Vis. Sci., 2018;59(13):5398-5407.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. VEGF vascularization pathway in human intervertebral disc does not change during the disc degeneration process
    Authors: S Capossela, A Bertolo, K Gunasekera, T Pötzel, M Baur, JV Stoyanov
    BMC Res Notes, 2018;11(1):333.
    Species: Bovine
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Ferrochelatase is a therapeutic target for ocular neovascularization
    Authors: HD Basavaraja, RS Sulaiman, X Qi, T Shetty, S Sheik Pran, KL Sishtla, B Lee, J Quigley, S Alkhairy, CM Briggs, K Gupta, B Tang, M Shadmand, MB Grant, ME Boulton, SY Seo, TW Corson
    EMBO Mol Med, 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Placental soluble fms-like tyrosine kinase expression in small for gestational age infants and risk for adverse outcomes
    Authors: M Spiel, S Salahuddin, E Pernicone, Z Zsengeller, A Wang, AM Modest, SA Karumanchi, JL Hecht
    Placenta, 2017;52(0):10-16.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Analysis of angiogenesis related factors in glioblastoma, peritumoral tissue and their derived cancer stem cells.
    Authors: Alessio D'alessio, Gabriella Proietti, Gina Lama, Filippo Biamonte, Libero Lauriola, Umberto Moscato, Angelo Vescovi, Annunziato Mangiola, Cristiana Angelucci, Gigliola Sica
    Oncotarget, 2016;0(0):1949-2553.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. Full-length human placental sFlt-1-e15a isoform induces distinct maternal phenotypes of preeclampsia in mice.
    Authors: Szalai G, Romero R, Chaiworapongsa T, Xu Y, Wang B, Ahn H, Xu Z, Chiang P, Sundell B, Wang R, Jiang Y, Plazyo O, Olive M, Tarca A, Dong Z, Qureshi F, Papp Z, Hassan S, Hernandez-Andrade E, Than N
    PLoS ONE, 2015;10(4):e0119547.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration.
    Authors: Chanakira A, Kir D, Barke R, Santilli S, Ramakrishnan S, Roy S
    PLoS ONE, 2015;10(9):e0138587.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. The effect of vascular endothelial growth factor on osteoclastogenesis in rheumatoid arthritis.
    Authors: Kim H, Kim K, Kim B, Cho M, Lee S
    PLoS ONE, 2015;10(4):e0124909.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Merkel cell carcinoma expresses vasculogenic mimicry: demonstration in patients and experimental manipulation in xenografts.
    Authors: Lezcano C, Kleffel S, Lee N, Larson A, Zhan Q, DoRosario A, Wang L, Schatton T, Murphy G
    Lab Invest, 2014;94(10):1092-102.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Elastase induces lung epithelial cell autophagy through placental growth factor: a new insight of emphysema pathogenesis.
    Authors: Hou H, Cheng S, Chung K, Kuo M, Yeh C, Chang B, Lu H, Wang H, Yu C
    Autophagy, 2014;10(9):1509-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling.
    Authors: Shvartsman, Dmitry, Storrie-White, Hannah, Lee, Kangwon, Kearney, Cathal, Brudno, Yevgeny, Ho, Nhi, Cezar, Christin, McCann, Corey, Anderson, Erin, Koullias, John, Tapia, Juan Car, Vandenburgh, Herman, Lichtman, Jeff W, Mooney, David J
    Mol Ther, 2014;22(7):1243-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  14. Melanoma spheroid formation involves laminin-associated vasculogenic mimicry.
    Authors: Larson A, Lee C, Lezcano C, Zhan Q, Huang J, Fischer A, Murphy G
    Am J Pathol, 2014;184(1):71-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  15. Attenuation of VEGFR-2 expression by sFlt-1 and low oxygen in human placenta.
    Authors: Nevo, Ori, Lee, Dennis K, Caniggia, Isabella
    PLoS ONE, 2013;8(11):e81176.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. Pancreatic-carcinoma-cell-derived pro-angiogenic factors can induce endothelial-cell differentiation of a subset of circulating CD34+ progenitors.
    Authors: Vizio, Barbara, Biasi, Fiorella, Scirelli, Tiziana, Novarino, Anna, Prati, Adriana, Ciuffreda, Libero, Montrucchio, Giuseppe, Poli, Giuseppe, Bellone, Graziell
    J Transl Med, 2013;11(0):314.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  17. gamma-Secretase and presenilin mediate cleavage and phosphorylation of vascular endothelial growth factor receptor-1.
    Authors: Cai J, Chen Z, Ruan Q, Han S, Liu L, Qi X, Boye SL, Hauswirth WW, Grant MB, Boulton ME
    J. Biol. Chem., 2012;286(49):42514-23.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Proximity Ligation Assay (PLA)
  18. Differential expression of Vegfr-2 and its soluble form in preeclampsia.
    Authors: Munaut C, Lorquet S, Pequeux C, Coulon C, Le Goarant J, Chantraine F, Noel A, Goffin F, Tsatsaris V, Subtil D, Foidart JM
    PLoS ONE, 2012;7(3):e33475.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot
  19. Glyceryl trinitrate inhibits hypoxia-induced release of soluble fms-like tyrosine kinase-1 and endoglin from placental tissues.
    Authors: Barsoum IB, Renaud SJ, Graham CH
    Am. J. Pathol., 2011;178(6):2888-96.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Vascular endothelial growth factor as an autocrine survival factor for retinal pigment epithelial cells under oxidative stress via the VEGF-R2/PI3K/Akt.
    Authors: Byeon SH, Lee SC, Choi SH, Lee HK, Lee JH, Chu YK, Kwon OW
    Invest. Ophthalmol. Vis. Sci., 2010;51(2):1190-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.
    Authors: Lee HS, Myers A, Kim J
    Am. J. Respir. Crit. Care Med., 2009;180(11):1056-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
    Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
    Am. J. Pathol., 2006;169(4):1167-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro.
    Authors: Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L
    Chem. Biol., 2006;13(5):549-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals.
    Authors: Akintoye SO, Lam T, Shi S, Brahim J, Collins MT, Robey PG
    Bone, 2006;38(6):758-68.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
    Authors: Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW
    Proc. Natl. Acad. Sci. U.S.A., 2005;102(29):10212-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. All three receptors for vascular endothelial growth factor (VEGF) are expressed on B-chronic lymphocytic leukemia (CLL) cells.
    Authors: Bairey O, Boycov O, Kaganovsky E, Zimra Y, Shaklai M, Rabizadeh E
    Leuk. Res., 2004;28(3):243-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  28. PEDF derived from glial Muller cells: a possible regulator of retinal angiogenesis.
    Authors: Eichler W, Yafai Y, Keller T, Wiedemann P, Reichenbach A
    Exp. Cell Res., 2004;299(1):68-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  29. Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
    Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V
    Blood, 2003;102(2):638-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Reviews for Human VEGFR1/Flt-1 Antibody

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Human VEGF R1/Flt-1 Antibody
By Anonymous on 08/08/2018
Application: ICC/IF Sample Tested: Embryonic brain Species: Mouse

transgenic mouse line -conditional induction of human sFlt1


Human VEGF R1/Flt-1 Antibody
By Anonymous on 07/08/2016
Application: ICC/IF Sample Tested: CT26 mouse colorectal cancer cell line,Human liver cancer cell line Species: Human