HuR/ELAVL1 Antibody (9Q6L3)
Novus Biologicals | Catalog # NBP3-15820
Recombinant Monoclonal Antibody
![Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]](https://resources.rndsystems.com/images/products/HuR-ELAVL1-Antibody-9Q6L3-Western-Blot-NBP3-15820-img0008.jpg "Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9Q6L3 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR/ELAVL1 (Q15717). MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HuR/ELAVL1 Antibody (9Q6L3)
Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]
Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] - Western blot analysis of extracts of various cell lines, using HuR/ELAVL1 antibody (NBP3-15820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Knockout Validated: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]](https://resources.rndsystems.com/images/products/HuR-ELAVL1-Antibody-9Q6L3-Knockout-Validated-NBP3-15820-img0001.jpg "Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]")
Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]
Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] - Western blot analysis of extracts from normal (control) and HuR/ELAVL1 knockout (KO) 293T cells, using HuR/ELAVL1 antibody (NBP3-15820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] -
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] -Human colon carcinoma using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] -
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] -Human kidney using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] -
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] -Human tonsil using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] -
Immunohistochemistry: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] -
Immunohistochemistry: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunocytochemistry/ Immunofluorescence: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] -
Immunocytochemistry/ Immunofluorescence: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Confocal imaging of U-2 OS cells using [KO Validated] HuR/ELAVL1 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Immunocytochemistry/ Immunofluorescence: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] -
Immunocytochemistry/ Immunofluorescence: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Confocal imaging of NIH/3T3 cells using [KO Validated] HuR/ELAVL1 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Immunohistochemistry: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] -
Immunohistochemistry: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] -
Immunohistochemistry: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunoprecipitation: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] -
Immunoprecipitation: HuR/ELAVL1 Antibody (9Q6L3) [HuR/ELAVL1] - Immunoprecipitation analysis of 300 ug extracts of 293T cells using 3 ug HuR/ELAVL1 antibody. Western blot was performed from the immunoprecipitate using HuR/ELAVL1 antibody at a dilution of 1:1000.Applications for HuR/ELAVL1 Antibody (9Q6L3)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunocytochemistry/ Immunofluorescence
1:100 - 1:1000
Immunohistochemistry
1:200 - 1:2000
Immunohistochemistry-Paraffin
1:200 - 1:2000
Immunoprecipitation
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Western Blot
1:1000 - 1:6000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HuR/ELAVL1
Alternate Names
ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R), ELAV-like protein 1, embryonic lethal, abnormal vision, drosophila, homolog-like 1, Hu antigen R, Hua, Hu-antigen R, HuR, HURELAV1, MelG
Gene Symbol
ELAVL1
Additional HuR/ELAVL1 Products
Product Documents for HuR/ELAVL1 Antibody (9Q6L3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HuR/ELAVL1 Antibody (9Q6L3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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