Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Dot Blot

Label

Alkaline Phosphatase

Antibody Source

Polyclonal Goat IgG
View all IgA/IgG/IgM (H+L) Secondary Antibodies »

Product Specifications

Immunogen

Human IgG, IgA and IgM whole molecule

Specificity

This reagent is suitable for the detection of all human immunoglobulin isotypes, subclasses and chain combinations.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Goat Serum

Scientific Data Images

Dot Blot: Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody [Alkaline Phosphatase] [NBP1-72622] - Antigen: Row 1 - Human IgG Row 2 - Human IgA Row 3 - Human IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Human IgG IgA IgM Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT. Blocked with blocking buffer for 60 min at RT.

Applications

Application
Recommended Usage

ELISA

1:2000 - 1:10000

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:3000
Application Notes
This product has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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