Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Fluorophore-linked immunosorbent assay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Goat IgG
View all IgA/IgG/IgM (H+L) Secondary Antibodies »

Product Specifications

Immunogen

Human IgG, IgA and IgM whole molecules

Specificity

This reagent is suitable for the detection of all Human isotypes and chain combinations.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG, Human IgA and Human IgM

Scientific Data Images for Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody [FITC]

Dot Blot: Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody [FITC] [NBP1-73307] - Dot Blot of Goat anti-Human IgG IgA IgM Antibody Fluorescein Conjugated. Antigen: Row 1 - Human IgG Row 2 - Human IgA Row 3 - Human IgM. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Human IgG IgA IgM Antibody Fluorescein Conjugated at 1:1,000 for 60 min at RT. Blocked with blocking buffer for 60 min at RT.
Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody [FITC]

Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody [FITC]

Dot Blot of Goat anti-Human IgG IgA IgM Antibody Fluorescein Conjugated. Antigen: Row 1 - Human IgG Row 2 - Human IgA Row 3 - Human IgM. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody [FITC]at 1:1,000 for 60 min at RT. Block for 60 min at RT.

Applications for Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody [FITC]

Application
Recommended Usage

Flow Cytometry

1:500 - 1:2500

Fluorophore-linked immunosorbent assay

1:10000 - 1:50000

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:1000-1:2500

Western Blot

1:100 - 1:250
Application Notes
This product has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Product Documents for Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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