Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody

Dot Blot: Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody [NBP1-72757] - Dot Blot of Goat anti-Human IgG IgA IgM Antibody Fluorescein Conjugated. Antigen: Row 1 - Human IgG Row 2 - Human IgA Row 3 - Human IgM. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Human IgG IgA IgM Antibody Fluorescein Conjugated at 1:1,000 for 60 min at RT. Blocked with blocking buffer for 60 min at RT. Image from the FITC version of this antibody.

Dot Blot: Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody [NBP1-72757] - Antigen: Row 1 - Human IgG Row 2 - Human IgA Row 3 - Human IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Human IgG IgA IgM Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT. Blocked with blocking buffer for 60 min at RT. Image from the Alkaline Phosphatase version of this antibody.

ELISA: Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody [NBP1-72757]

ELISA: Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody [NBP1-72757] - ELISA Results of purified Goat anti-Human IgA/IgG/IgM (H+L) Secondary antibody tested against purified Human IgG, IgA, IgM. The working dilution for Anti-Human IgG, IgA, IgM is 1:20,000. Each well was coated in duplicate with 1.0 ug of Human IgG protein [Red Line], Human IgA [Green Line], Human IgM [Blue Line], Human IgG/A/M [Purple Line]. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Donkey Anti-Goat IgG HRP conjugated and TMBE-1000 substrate.