IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-48001

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Rabbit

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # IGF1/1020

Format

Azide and BSA Free
View all IGF-I/IGF-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human IGF-I/IGF-1 protein (Uniprot: P05019)

Localization

Cytoplasmic (Secreted)

Specificity

This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44968).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free [NBP2-48001]

Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free [NBP2-48001]

Immunocytochemistry/Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free [NBP2-48001] - Immunofluorescence Analysis of HeLa cells labeling IGF-1 with IGF-I/IGF-1 Antibody (IGF1/1020) labeled with CF647R (Green). Nuclei are counter stained with DAPI (Blue).

Applications for IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunofluorescence

1 - 2 ug/ml
Application Notes
In-vitro Neutralization of Biological Activity of IGF-I/IGF-1: Order Ab without Azide.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: IGF-I/IGF-1

Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Gene Symbol

IGF1

Additional IGF-I/IGF-1 Products

Product Documents for IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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