IkB-alpha [p Ser36] Antibody (23GB1300)

Name: IkB-alpha [p Ser36] Antibody (23GB1300)
Brand: Novus Biologicals
SKU: NBP3-50940
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-50940

Submit a review

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1300

View all IkB-alpha Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-IkB-alpha (S36) (Uniprot: P25963)

Modification

p Ser36

Specificity

This antibody detects endogenous levels of NFKBIA only when phosphorylated at S36

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IkB-alpha [p Ser36] Antibody (23GB1300)

Immunocytochemistry/ Immunofluorescence: IkB-alpha [p Ser36] Antibody (23GB1300)[NBP3-50940] -

Immunocytochemical staining of HepG2 cells with IkB-alpha antibody (1:1,000). Nuclei were stained blue with DAPI; IkB-alpha was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.

Western Blot: IkB-alpha [p Ser36] Antibody (23GB1300)[NBP3-50940] -

Western blotting analysis using IkB-alpha (S36) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for IkB-alpha [p Ser36] Antibody (23GB1300)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

Additional IkB-alpha Products

Product Documents for IkB-alpha [p Ser36] Antibody (23GB1300)

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IkB-alpha [p Ser36] Antibody (23GB1300)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.