IkB-epsilon Antibody - BSA Free

Name: IkB-epsilon Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-87762
Price: 539 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-87762

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AEESQYDSGIESLRSLRSLPESTSAPASGPSDGSPQPCTHPPGPVKEPQEKEDADGERADSTYGSSSLTYTLSLLGGPEAEDPAPRLPLPHVGALSPQQLEALTYISEDGDTLVHLAVIHEAPAVLLCCLALLPQEVLDIQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IkB-epsilon Antibody - BSA Free

Western Blot: IkB-epsilon Antibody [NBP1-87762]

Western Blot: IkB-epsilon Antibody [NBP1-87762] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Immunocytochemistry/ Immunofluorescence: IkB-epsilon Antibody [NBP1-87762]

Immunocytochemistry/Immunofluorescence: IkB-epsilon Antibody [NBP1-87762] - Staining of human cell line U-2 OS shows localization to nucleus, nucleoli fibrillar center & cytosol.

Immunohistochemistry-Paraffin: IkB-epsilon Antibody [NBP1-87762]

Immunohistochemistry-Paraffin: IkB-epsilon Antibody [NBP1-87762] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.

Immunohistochemistry-Paraffin: IkB-epsilon Antibody [NBP1-87762]

Immunohistochemistry-Paraffin: IkB-epsilon Antibody [NBP1-87762] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: IkB-epsilon Antibody [NBP1-87762]

Immunohistochemistry-Paraffin: IkB-epsilon Antibody [NBP1-87762] - Staining of human lymph node shows strong cytoplasmic positivity.

Immunohistochemistry-Paraffin: IkB-epsilon Antibody [NBP1-87762]

Immunohistochemistry-Paraffin: IkB-epsilon Antibody [NBP1-87762] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Chromatin Immunoprecipitation ChIP: IkB-epsilon Antibody - BSA Free

ChIP-Exo-Seq composite graph for Anti-IkB-epsilon tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.

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Applications for IkB-epsilon Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:100-1:500

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IkB-epsilon

The transcription factor NFkappaB is retained in the cytoplasm in an inactive form by the inhibitory protein IkappaB. Activation of NFkappaB requires that IkappaB be phosphorylated on specific serine residues, which results in targeted degradation of IkappaB. IkappaB kinase alpha (IKKalpha), previously designated CHUK, interacts with IkappaB-alpha and specifically phosphorylates IkappaB-alpha on the sites that trigger its degradation, Serines 32 and 36. IKKalpha appears to be critical for NFkappaB activation in response to proinflammatory cytokines. Phosphorylation of IkappaB by IKKalpha is stimulated by the NFkappaB inducing kinase (NIK), which itself is a central regulator for NFkappaB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKalpha, IKKbeta and IKKgamma (also designated NEMO), and each appear to make essential contributions to IkappaB phosphorylation.

Long Name

I-kappa-B-epsilon

Alternate Names

IkBepsilon, NFKBIE

Gene Symbol

NFKBIE

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Product Specific Notices for IkB-epsilon Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.