IL-17/IL-17A Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-76337
![Western Blot: IL-17/IL-17A AntibodyBSA Free [NBP1-76337]](https://resources.rndsystems.com/images/products/IL-17-IL-17A-Antibody-Western-Blot-NBP1-76337-img0013.jpg "Western Blot: IL-17/IL-17A AntibodyBSA Free [NBP1-76337]")
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Species Reactivity
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Applications
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Cited:
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Immunogen
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Scientific Data Images for IL-17/IL-17A Antibody - BSA Free
Western Blot: IL-17/IL-17A AntibodyBSA Free [NBP1-76337]
Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Analysis of 1 ng of recombinant human IL-17 with IL-17 antibody at 1 ug/ml.![Western Blot: IL-17/IL-17A AntibodyBSA Free [NBP1-76337]](https://resources.rndsystems.com/images/products/IL-17-IL-17A-Antibody-Western-Blot-NBP1-76337-img0012.jpg "Western Blot: IL-17/IL-17A AntibodyBSA Free [NBP1-76337]")
Western Blot: IL-17/IL-17A AntibodyBSA Free [NBP1-76337]
Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Loading: 10 ug of lysates per lane. Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 min Lane 1: 1 ug/mL Lane 2: 2 ug/mL
Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] -
Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Colon Tissue. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IL-17/IL-17A antibody at 2 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] -
Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Heart Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart issue labeling IL-17/IL-17A with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] -
Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Human Tonsil Tissue.Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-17/IL-17A antibody at 5 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] -
Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Thymus Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17/IL-17A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] -
Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Human Lymph Node.Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-IL-17/IL-17A antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] -
Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse A-20 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse A-20 Cells labeling IL-17/IL-17A with at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).Applications for IL-17/IL-17A Antibody - BSA Free
ELISA
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
Formulation, Preparation, and Storage
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Formulation
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Background: IL-17/IL-17A
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Additional IL-17/IL-17A Products
Product Documents for IL-17/IL-17A Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for IL-17/IL-17A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for IL-17/IL-17A Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for IL-17/IL-17A Antibody - BSA Free
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Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
A:
Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
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Q: I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
A:
Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).
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Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
A:
Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
-
Q: I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
A:
Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).
Associated Pathways
Innate Lymphoid Cell Differentiation Pathways
Th17 Differentiation Pathway
