IL-1ra/IL-1F3/IL1RN Antibody - Azide Free

Western Blot: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IL-1ra/IL-1F3/IL1RN antibody (NBP1-32568) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Western Blot: IL-1ra/IL-1F3/IL1RN Antibody - Azide Free [NBP1-32568] -

Effects of the ELF4/IL1RN axis on inflammation symptoms in mice intestines. (A) RT-qPCR was used to detect the mRNA levels of ELF4 and IL1RN in intestinal tissues of mice from each group; (B) Western blot was used to detect the protein levels of ELF4 and IL1RN in intestinal tissues of mice from each group; (C) Flow cytometry was used to detect the proportions of M1 and M2 cells in intestinal tissues of mice from each group; (D) Western blot was used to detect the protein levels of M1 marker iNOS and M2 markers Arg1 and CD163 in intestinal tissues of mice from each group; (E) The DAI scores of mice in each group were recorded; (F) H&E staining was used to evaluate colonic tissue injury in mice from each group; (G) ELISA was used to measure the levels of inflammatory factors Treml, IL-6, TNF-alpha, and IL-1 beta in the serum of mice from each group; (H) Flow cytometry was used to detect the proportions of TH17 and Treg cells in intestinal tissues of mice from each group, as well as the TH17/Treg ratio. * indicates P < 0.05 compared with oe-NC + sh-NC, and # indicates P < 0.05 compared with oe-ELF4+sh-NC. All cell experiments were repeated three times. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38022496), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Knockdown Validated: IL-1ra/IL-1F3/IL1RN Antibody - Azide Free [NBP1-32568] -

The impact of ELF4/IL1RN axis on macrophages and mouse intestinal epithelial cells. (A) The mRNA levels of ELF4 and IL1RN in each group of BMDM cells were detected by RT-qPCR; (B) The protein levels of ELF4 and IL1RN in each group of BMDM cells were detected by Western blot; (C) The polarization of M1 and M2 in each group of BMDM cells was detected by flow cytometry; (D) The protein levels of M1 marker iNOS and M2 markers Arg1 and CD163 in each group of BMDM cells were detected by Western blot; (E) The apoptosis of each group of MODE-K cells was detected by flow cytometry (The sum of the blue quadrant areas represents the apoptotic rate); (F) The protein levels of apoptosis factors Bcl-2, Bax, cleaved caspase-1, pro caspase-1,caspase 3 and Cytochrome c in each group of MODE-K cells were detected by Western blot; (G) The levels of inflammatory factors Treml, IL-6, TNF-alpha, and IL-1 beta in each group of MODE-K cells were detected by ELISA. * indicates P < 0.05 compared with oe-NC + sh-NC, and # indicates P < 0.05 compared with oe-ELF4 + sh-NC. All cell experiments were repeated three times. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38022496), licensed under a CC-BY license. Not internally tested by Novus Biologicals.