Human/mouse IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and human IL-29 (IFN-lambda 1) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and type I IFN family (15 - 19% aa sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28 shares 61%, 62%, and 52% aa identity with human IL-28A, IL-28B, and IL-29, respectively.
New
Recombinant Bovine IL-28B/IFN-lambda 3 Protein
Novus Biologicals | Catalog # NBP3-57588
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Key Product Details
Applications
Western Blot, ELISA, Cell Culture
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Product Specifications
Description
Stable for up to 12 months from date of receipt at -20C. Stable for 3 months when stored in working aliquots with a carrier protein at -20C. Avoid repeated freeze/thaw cycles.
Purity
>98%, by SDS-PAGE
Endotoxin Level
No affinity tags & naturally endotoxin-free
Predicted Molecular Mass
19.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications
Enzyme-linked Immunosorbent Spot (ELISPOT)
ELISA Standard (Matched Pair) (ELISA(Sta))
ELISA Standard (Matched Pair) (ELISA(Sta))
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation, and Storage
NBP3-57588
| Formulation | Lyophilized in 1-5X PBS containing 10% Trehalose |
| Preservative | 10% Trehalose |
| Reconstitution | Reconstitute in sterile PBS with at least 0.1% carrier protein |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20C. Avoid freeze-thaw cycles. |
Calculators
Background: IL-28B/IFN-lambda 3
Long Name
Interleukin 28B
Alternate Names
IFN-lambda 3
Gene Symbol
IFNL3
Additional IL-28B/IFN-lambda 3 Products
Product Documents for Recombinant Bovine IL-28B/IFN-lambda 3 Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Recombinant Bovine IL-28B/IFN-lambda 3 Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- A Guide to the Colony Forming Cell Assay: Methods and Tips
- Cellular Response to Hypoxia Protocols
- Cultrex UltiMatrix BME Protocol
- Culture of Mouse Enteric Organoids using Cultrex Basement Membrane Extract
- Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
- Culturing Mouse Cortical Stem Cells: Expansion using the Neurosphere System
- Culturing Rat Cortical Stem Cells: Expansion using the Monolayer System
- Culturing Rat Cortical Stem Cells: Expansion using the Neurosphere System
- Definitive Endoderm Differentiation of BG01V Human Embryonic Stem Cells
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- General Support Buffers
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Protocol for CEPT Cocktail Kit
- Protocol for Cell Counting Kit-8
- Protocol for Culturing Embryonic Chick Dorsal Root Ganglion Neurons
- Protocol for Culturing Embryonic Rat Spinal Motor Neurons
- Protocol for Culturing Rat Hippocampal Neurons
- Protocol for Culturing Rat Microglia
- Protocol for MortaLIVE™ Non-Toxic Dye
- Protocol for the Differentiation and Characterization of Human Th1 Cells
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Rat Cortical Stem Cell Culture Expansion Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- The Human Colony Forming Cell (CFC) Assay using Methylcellulose-based Media
- The Mouse Colony Forming Cell (CFC) Assay using Methylcellulose-based Media
- The Mouse Pre-B Colony Forming Cell (CFC) Assay Using Methylcellulose-based Media
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Use of Cultrex Basement Membrane Extract to Improve Take and Growth of Xenografts in Mice
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
