IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free

Name: IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-37354
Price: 189 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-37354

Submit a review

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 8E11C5

Format

BSA Free

View all IL-3R alpha Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human IL-3R alpha/CD123 (AA: 200-305) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

43.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free

Western Blot: IL-3R alpha/CD123 Antibody (8E11C5)BSA Free [NBP2-37354]

Western Blot: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis using IL3RA mAb against HEK293 (1) and IL3RA (AA: 200-305)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/ Immunofluorescence: IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free [NBP2-37354]

Immunocytochemistry/Immunofluorescence: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis of Hela cells using IL3RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free [NBP2-37354]

Immunohistochemistry: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis of lung cancer tissues using IL3RA mouse mAb with DAB staining.

Flow Cytometry: IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free [NBP2-37354]

Flow Cytometry: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis of Hela cells using IL3RA mouse mAb (green) and negative control (red).

Western Blot: IL-3R alpha/CD123 Antibody (8E11C5)BSA Free [NBP2-37354]

Western Blot: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis using IL3RA mAb against human IL3RA recombinant protein. (Expected MW is 38.3 kDa)

Immunohistochemistry-Paraffin: IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free [NBP2-37354]

Immunohistochemistry-Paraffin: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis of ovarian cancer tissues using IL3RA mouse mAb with DAB staining.

ELISA: IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free [NBP2-37354]

ELISA: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

View 4 more images

Applications for IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Application Notes

This antibody is Cytof ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-3R alpha

IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Gene Symbol

IL3RA

Additional IL-3R alpha Products

Product Documents for IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.