iNOS Antibody (4E5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80814

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4E5

Format

Azide and BSA Free
View all iNOS Primary Antibodies »

Product Specifications

Immunogen

iNOS Antibody (4E5) was made to a purified recombinant fragment of human iNOS (C-terminus) expressed in E. coli. [UniProt# P35228]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for iNOS Antibody (4E5) - Azide and BSA Free

Western Blot: iNOS Antibody (4E5)Azide and BSA Free [NBP2-80814]

Western Blot: iNOS Antibody (4E5)Azide and BSA Free [NBP2-80814]

Western Blot: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814] - Analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5) and MCF-7 (6) cell lysate. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814]

Immunohistochemistry-Paraffin: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814]

Immunohistochemistry-Paraffin: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814] - Analysis of paraffin-embedded liver cancer tissues using iNOS mouse mAb with DAB staining. Image from the standard format of this antibody.
Flow Cytometry: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814]

Flow Cytometry: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814]

Flow Cytometry: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814] - An intracellular stain was performed on A549 cells with NBP2-22119PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin (PE).
Western Blot: iNOS Antibody (4E5)Azide and BSA Free [NBP2-80814]

Western Blot: iNOS Antibody (4E5)Azide and BSA Free [NBP2-80814]

Western Blot: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814] - Analysis using iNOS mAb against human iNOS (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa) Image from the standard format of this antibody.
Immunohistochemistry: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814]

Immunohistochemistry: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814]

Immunohistochemistry: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814] - Analysis of paraffin-embedded breast cancer tissues using iNOS mouse mAb with DAB staining. Image from the standard format of this antibody.
Flow Cytometry: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814]

Flow Cytometry: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814]

Flow Cytometry: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814] - An intracellular stain was performed on A549 cells with NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 1 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to allophycocyanin (APC).
ELISA: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814]

ELISA: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814]

ELISA: iNOS Antibody (4E5) - Azide and BSA Free [NBP2-80814] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng). Image from the standard format of this antibody.

Applications for iNOS Antibody (4E5) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

reported in scientific literature (PMID 29891729)

Western Blot

1:500-1:2000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: iNOS

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

Long Name

Inducible Nitic Oxide Synthase

Alternate Names

NOS2, NOS2A

Gene Symbol

NOS2

Additional iNOS Products

Product Documents for iNOS Antibody (4E5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for iNOS Antibody (4E5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for iNOS Antibody (4E5) - Azide and BSA Free

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  • Q: We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?

    A: We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

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