Integrin alpha 2/CD49b Antibody - BSA Free

Name: Integrin alpha 2/CD49b Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP3-03851
Price: 199 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-03851

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all Integrin alpha 2/CD49b Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 30-162 of human Integrin alpha 2/CD49b (NP_002194.2). YNVGLPEAKIFSGPSSEQFGYAVQQFINPKGNWLLVGSPWSGFPENRMGDVYKCPVDLSTATCEKLNLQTSTSIPNVTEMKTNMSLGLILTRNMGTGGFLTCGPLWAQQCGNQYYTTGVCSDISPDFQLSASF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin alpha 2/CD49b Antibody - BSA Free

Western Blot: Integrin alpha 2/CD49b AntibodyAzide and BSA Free [NBP3-03851]

Western Blot: Integrin alpha 2/CD49b Antibody [NBP3-03851] - Western blot analysis of extracts from normal (control) and Integrin alpha 2/CD49b knockout (KO) 293T cells, using Integrin alpha 2/CD49b antibody (NBP3-03851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry/ Immunofluorescence: Integrin alpha 2/CD49b Antibody - Azide and BSA Free [NBP3-03851]

Immunocytochemistry/Immunofluorescence: Integrin alpha 2/CD49b Antibody [NBP3-03851] - Analysis of U2OS cells using Integrin alpha 2/CD49b antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody - Azide and BSA Free [NBP3-03851]

Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody [NBP3-03851] - Immunohistochemistry of paraffin-embedded human tonsil using [KO Validated] Integrin alpha 2/CD49b Rabbit pAb (NBP3-03851) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western Blot: Integrin alpha 2/CD49b AntibodyAzide and BSA Free [NBP3-03851]

Western Blot: Integrin alpha 2/CD49b Antibody [NBP3-03851] - Western blot analysis of extracts of various cell lines, using Integrin alpha 2/CD49b antibody (NBP3-03851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody - Azide and BSA Free [NBP3-03851]

Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody [NBP3-03851] - Immunohistochemistry of paraffin-embedded human lung cancer using [KO Validated] Integrin alpha 2/CD49b Rabbit pAb (NBP3-03851) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody - Azide and BSA Free [NBP3-03851]

Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody [NBP3-03851] - Immunohistochemistry of paraffin-embedded human placenta using [KO Validated] Integrin alpha 2/CD49b Rabbit pAb (NBP3-03851) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Applications for Integrin alpha 2/CD49b Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Integrin alpha 2/CD49b

Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. They are responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells.

Alternate Names

CD49b, ITGA2, VLA-2 alpha

Gene Symbol

ITGA2

Additional Integrin alpha 2/CD49b Products

Product Documents for Integrin alpha 2/CD49b Antibody - BSA Free

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Product Specific Notices for Integrin alpha 2/CD49b Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.