Q: I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

A: I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Integrin beta 1/CD29 Antibody (3B6) - BSA Free

Novus Biologicals | Catalog # NBP2-22191

(1)

Citations (2)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Porcine, Monkey, Primate

Cited:

Human, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Cited:

CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 3B6

Format

BSA Free

View all Integrin beta 1/CD29 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Integrin beta 1/CD29 expressed in E. Coli.

Reactivity Notes

Use in Porcine reported in scientific literature (PMID:32211117).

Localization

Membrane

Marker

Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

88.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin beta 1/CD29 Antibody (3B6) - BSA Free

Western Blot: Integrin beta 1/CD29 Antibody (3B6)BSA Free [NBP2-22191]

Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mouse mAb against Hela (1), HepG2 (2), A549 (3), Jurkat(4), L1210 (5) and Cos7 (6) cell lysate.

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody (3B6) - BSA Free [NBP2-22191]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using Integrin beta 1 mouse mAb with DAB staining.

Flow Cytometry: Integrin beta 1/CD29 Antibody (3B6) - BSA Free [NBP2-22191]

Flow Cytometry: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Flow cytometric analysis of Hela cells using Integrin beta 1 mouse mAb (green) and negative control (red).

Western Blot: Integrin beta 1/CD29 Antibody (3B6)BSA Free [NBP2-22191]

Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mAb against human ITGB1 (AA: 50-270) recombinant protein. (Expected MW is 50.6 kDa)

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody (3B6) - BSA Free [NBP2-22191]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Integrin beta 1 mouse mAb with DAB staining.

ELISA: Integrin beta 1/CD29 Antibody (3B6) - BSA Free [NBP2-22191]

ELISA: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

CyTOF-ready: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Analysis of Integrin beta 1/CD29 antibody on human tissue. Integrin beta 1/CD29 (green). Scale, 50um. Image from verified customer review.

View 4 more images

Applications for Integrin beta 1/CD29 Antibody (3B6) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Application Notes

This antibody is Cytof ready.

Reviewed Applications

Read 1 review rated 4 using NBP2-22191 in the following applications:

CyTOF (1 Review)

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 1/CD29

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.

Alternate Names

CD29, ITGB1

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody (3B6) - BSA Free

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Integrin beta 1/CD29 Antibody (3B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules

Adhesion Molecules on Endothelial Cells

Adipose-derived Stem Cell Markers

Cancer Biomarkers

Cardiac Stem Cell Markers

Epithelial Cell Polarity

Integrins and Associated Molecules

Mesenchymal Stem Cell Markers

Skin Stem Cell Markers

Citations for Integrin beta 1/CD29 Antibody (3B6) - BSA Free

Customer Reviews for Integrin beta 1/CD29 Antibody (3B6) - BSA Free (1)

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Integrin beta 1/CD29 Antibody (3B6)

Name: Anonymous

Application: CyTOF

Sample Tested: Human tissue origin

Species: Human

Verified Customer | Posted 01/04/2022

Integrin beta-1/CD29 (green). Scale, 50um.

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Protocols

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FAQs for Integrin beta 1/CD29 Antibody (3B6) - BSA Free

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Q: I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

A: I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.