Islet-1 Antibody (2E7) - Azide and BSA Free

Novus Biologicals | Catalog # H00003670-M05

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Functional

Cited:

Western Blot, Immunoprecipitation, Chemotaxis

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E7

Format

Azide and BSA Free
View all Islet-1 Primary Antibodies »

Product Specifications

Immunogen

ISL1 (NP_002193, 63 a.a. ~ 159 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEP

Localization

Nuclear, occasionally cytoplasmic

Specificity

ISL1 - ISL1 transcription factor, LIM/homeodomain, (islet-1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Islet-1 Antibody (2E7) - Azide and BSA Free

Western Blot: Islet-1 Antibody (2E7) [H00003670-M05]

Western Blot: Islet-1 Antibody (2E7) [H00003670-M05]

Western Blot: Islet-1 Antibody (2E7) [H00003670-M05] - Analysis of ISL1 expression in PC-12 (Cat # L012V1).
Immunocytochemistry/ Immunofluorescence: Islet-1 Antibody (2E7) [H00003670-M05]

Immunocytochemistry/ Immunofluorescence: Islet-1 Antibody (2E7) [H00003670-M05]

Immunocytochemistry/Immunofluorescence: Islet-1 Antibody (2E7) [H00003670-M05] - Analysis of monoclonal antibody to ISL1 on HeLa cell. Antibody concentration 10 ug/ml

Applications for Islet-1 Antibody (2E7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been useful for IF and ELISA. IP usage reported in scientific literature (PMID: 25070240). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 26635867).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Islet-1

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Alternate Names

ISL1, Islet1

Entrez Gene IDs

3670 (Human)

Gene Symbol

ISL1

OMIM

600366 (Human)

UniProt

NP_002193 (Human)

Additional Islet-1 Products

Product Documents for Islet-1 Antibody (2E7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Islet-1 Antibody (2E7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Islet-1 Antibody (2E7) - Azide and BSA Free

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Protocols

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FAQs

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