JunD Antibody - BSA Free

Chromatin Immunoprecipitation: JunD Antibody [NBP2-22439] - JunD analysis using cross-linked chromatin from 1x10^6 HTC-IR rat hepatoma cells treated w/ insulin @ 0, 10, & 30 mins. IP using multiplex Matrix ChIP assay, 1 ul/100ul well volume of JunD pAb. Chromatin aliquots from ~1x10^5 cells used/ChIP pull-down. Quantitative PCR data: 4x using 1ul eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify exon-1 - exon-28 of LAMC1. PCR calibration curves made per primer pair from dilution series of sheared total genomic DNA. ChIP quantitation: signal relative to total amount of input chromatin. Results: mean +/- SEM for 3 experiments. Schematic representation of rat LAMC1 locus above data where boxes represent exons (black = translated regions, white = untranslated regions). Lines: zigzag represents an intron, & the straight represents upstream sequence. Regions amplified by LAMC1 primers represented by black bars. Data courtesy of the Innovators Program.