KAP1 [p Ser824] Antibody

Novus Biologicals | Catalog # NB100-2350

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all KAP1 Primary Antibodies »

Product Specifications

Immunogen

This KAP1 [p Ser824] Antibody was developed against mmunogen a synthetic phosphorylated peptide, which represents a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24248351). Rat reactivity reported in scientific literature (PMID: 27895165).

Modification

p Ser824

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB500-158, NB100-41429

Scientific Data Images for KAP1 [p Ser824] Antibody

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - MDA-MB-231 cells were treated with DMSO or Merbarone (100 uM) for 24 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. KAP1 [p Ser824] Antibody (NB100-2350) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: KAP1 [p Ser824] Antibody [NB100-2350]

Immunocytochemistry/ Immunofluorescence: KAP1 [p Ser824] Antibody [NB100-2350]

Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody [NB100-2350] - NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with EPE (left) or untreated (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight (R) 594 conjugated used at a dilution of 1:100.
Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350]

Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350]

Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - Human Phospho Kap1 (S824) by Immunohistochemistry. Sample: FFPE serial section of human lung cancer used at a dilution of 1:200 (1 ug/ml). Detection: DAB
Western Blot: KAP1 [p Ser824] Antibody [NB100-2350]

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350]

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (15 ug) from NIH 3T3 cellstreated with 100 uM etoposide (+) or mock treated (-).Antibodies: Affinity purified rabbit anti-Phospho KAP-1(S824) antibody used forWB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 30 seconds. Lower panel shows westernblot for total KAP-1 using rabbit anti-KAP-1 recombinantmonoclonal antibody at 1:1000.
Western Blot: KAP1 [p Ser824] Antibody [NB100-2350]

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350]

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (50 ug) from HEK293Tcells treated with 100 uM etoposide (+) or mock treated(-). Antibodies: Affinity purified rabbit anti-Phospho KAP-1(S824) antibody used forWB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 1 second. Lower panel shows westernblot for total KAP-1 using affinity purified rabbit anti KAP-1 antibody at 0.1 ug/ml.
Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350]

Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350]

Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - FFPE serial sections of asynchronous HeLa cells treated with EPE (left) anduntreated HeLa cells (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunoprecipitation: KAP1 [p Ser824] Antibody [NB100-2350]

Immunoprecipitation: KAP1 [p Ser824] Antibody [NB100-2350]

Immunoprecipitation: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) and rabbit anti-KAP-1 recombinant monoclonal antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Phospho KAP-1 (S824), Antibody was used at 0.04 ug/ml. To examine total KAP-1, the blot was stripped and then blotted with this antibody at 1:1000 (lower panel). Detection: Chemiluminescence with an exposure time of 1 second.Previous
KAP1 [p Ser824] Antibody

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] -

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - MAGE I regulates KAP1 gene binding, trimethylation of histone 3 on lysine 9, & gene repression in HEK293T cells.MAGE I expression decreases binding of KAP1, H3me3K9, & repression of the ID1 tumor suppressor gene (A, B, D). In contrast, MAGE I expression increases binding of KAP1, H3me3K9, & repression of mRNA & protein levels of the Ki67 gene (E, F, H, I, J, L). Note MAGE I binds to Ki67 gene sites but not ID1 gene sites (C, G, K). “M” denotes Mock transfection control. “A3” & “C2” denote MAGE-A3 & MAGE-C2, respectively. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/21876767), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
KAP1 [p Ser824] Antibody

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] -

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Truncated PPM1D impairs DNA damage response in mouse thymus. Expression of PPM1D mRNA analyzed by RT-qPCR in thymi of Ppm1d+/+, Ppm1dT/+ & Ppm1dT/T mice & normalized to GAPDH (n = 3) (A). Thymi from mice of indicated genotypes lysed & proteins separated by SDS-PAGE. Samples probed w/ antibody against PPM1D & importin-beta as a loading control. The empty & full arrowheads indicate the position of full-length & the C-terminally truncated PPM1D, respectively. (B). Cells from thymi from Ppm1d+/+ & Ppm1dT/+ mice analyzed by flow cytometry. Plotted are the counts of indicated populations as follows: double-negative T-cells (DN & DN1, DN2, DN3, DN4), double-positive T-cells (DP), CD8-positive T-cells (CD8+) & CD4-positive T-cells (CD4+) (n = 3) (C). The median size of thymus determined in Ppm1d +/+ (n = 11) & Ppm1dT/+ (n = 12) mice (D). A scheme of experimental setup in F-I. Mice exposed or not to a low dose of IR (3 Gy), sacrificed after 6 h & thymi & lymph nodes collected (E). Proteins isolated from thymi from mice of indicated genotypes exposed to mock or to IR probed w/ indicated antibodies by immunoblotting (F). Proteins isolated from inguinal lymph nodes from mice of indicated genotypes exposed to mock or to IR probed w/ indicated antibodies by immunoblotting (G). RNA isolated from thymi from mice in E analyzed by RT-qPCR. Expression of CDKN1Ap21 mRNA normalized to GAPDH. Statistical significance evaluated by two-tailed t-test, error bars indicate SD, n = 5 (H). RNA isolated from thymi from mice in D analyzed by RT-qPCR. Expression of PUMA mRNA normalized to GAPDH. Statistical significance evaluated by two-tailed t-test, error bars indicate SD, n = 5. * p <0.05; *** p < 0.0005; **** p < 0.0001 (I). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32927737), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
KAP1 [p Ser824] Antibody

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] -

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Truncated PPM1D impairs DNA damage response in mouse thymus. Expression of PPM1D mRNA analyzed by RT-qPCR in thymi of Ppm1d+/+, Ppm1dT/+ & Ppm1dT/T mice & normalized to GAPDH (n = 3) (A). Thymi from mice of indicated genotypes lysed & proteins separated by SDS-PAGE. Samples probed w/ antibody against PPM1D & importin-beta as a loading control. The empty & full arrowheads indicate the position of full-length & the C-terminally truncated PPM1D, respectively. (B). Cells from thymi from Ppm1d+/+ & Ppm1dT/+ mice analyzed by flow cytometry. Plotted are the counts of indicated populations as follows: double-negative T-cells (DN & DN1, DN2, DN3, DN4), double-positive T-cells (DP), CD8-positive T-cells (CD8+) & CD4-positive T-cells (CD4+) (n = 3) (C). The median size of thymus determined in Ppm1d +/+ (n = 11) & Ppm1dT/+ (n = 12) mice (D). A scheme of experimental setup in F-I. Mice exposed or not to a low dose of IR (3 Gy), sacrificed after 6 h & thymi & lymph nodes collected (E). Proteins isolated from thymi from mice of indicated genotypes exposed to mock or to IR probed w/ indicated antibodies by immunoblotting (F). Proteins isolated from inguinal lymph nodes from mice of indicated genotypes exposed to mock or to IR probed w/ indicated antibodies by immunoblotting (G). RNA isolated from thymi from mice in E analyzed by RT-qPCR. Expression of CDKN1Ap21 mRNA normalized to GAPDH. Statistical significance evaluated by two-tailed t-test, error bars indicate SD, n = 5 (H). RNA isolated from thymi from mice in D analyzed by RT-qPCR. Expression of PUMA mRNA normalized to GAPDH. Statistical significance evaluated by two-tailed t-test, error bars indicate SD, n = 5. * p <0.05; *** p < 0.0005; **** p < 0.0001 (I). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32927737), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for KAP1 [p Ser824] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:35031618) Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. KAP1 [p Ser824] antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-2350 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KAP1

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Long Name

KRAB-associated Protein 1

Alternate Names

KRIP-1, RNF96, TF1B, TIF1B, TRIM28

Entrez Gene IDs

10155 (Human)

Gene Symbol

TRIM28

UniProt

Q13263 (Human)

Additional KAP1 Products

Product Documents for KAP1 [p Ser824] Antibody

Certificate of Analysis

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Product Specific Notices for KAP1 [p Ser824] Antibody

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB500-158, NB100-41429

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KAP1 [p Ser824] Antibody

Customer Reviews for KAP1 [p Ser824] Antibody (1)

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  • KAP1 [p Ser824] Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: MDA-231 cell lysate
    Species: Human
    Verified Customer | Posted 10/28/2020
    Western Blot: MDA-MB-231 cells were treated with DMSO or Merbarone (100 μM) for 24 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. KAP1 [p Ser824] Antibody (NB100-2350) primary antibody: 1:1000, 4℃, overnight.
    KAP1 [p Ser824] Antibody NB100-2350

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