KLF4 Antibody (3A1) - BSA Free

Novus Biologicals | Catalog # NBP3-26180

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3A1 expressed in HEK293

Format

BSA Free
View all KLF4 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human KLF4 [UniProt O43474]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KLF4 Antibody (3A1) - BSA Free

KLF4 Antibody (3A1)

Immunoprecipitation: KLF4 Antibody (3A1) [NBP3-26180] -

Immunoprecipitation: KLF4 Antibody (3A1) [NBP3-26180] - Immunoprecipitating KLF4 in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26180 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26180 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).
KLF4 Antibody (3A1)

Immunohistochemistry: KLF4 Antibody (3A1) [NBP3-26180] -

Immunohistochemistry: KLF4 Antibody (3A1) [NBP3-26180] - Image of KLF4 Antibody (3A1) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
KLF4 Antibody (3A1)

Immunohistochemistry: KLF4 Antibody (3A1) [NBP3-26180] -

Immunohistochemistry: KLF4 Antibody (3A1) [NBP3-26180] - Image of KLF4 Antibody (3A1) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
KLF4 Antibody (3A1)

Western Blot: KLF4 Antibody (3A1) [NBP3-26180] -

Western Blot: KLF4 Antibody (3A1) [NBP3-26180] - Positive Western Blot detected in: Hela whole cell lysate, 293T whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, Brain tissue.
All lanes: KLF4 Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 55, 52, 46, 13, 7 kDa
Observed band size: 55 kDa
KLF4 Antibody (3A1)

Immunocytochemistry/Immunofluorescence: KLF4 Antibody (3A1) [NBP3-26180] -

Immunocytochemistry/Immunofluorescence: KLF4 Antibody (3A1) [NBP3-26180] - Staining of Hela Cells with KLF4 Antibody (3A1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for KLF4 Antibody (3A1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: KLF4

KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.

Long Name

Kruppel-Like Factor 4

Alternate Names

EZF

Gene Symbol

KLF4

Additional KLF4 Products

Product Documents for KLF4 Antibody (3A1) - BSA Free

Certificate of Analysis

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Product Specific Notices for KLF4 Antibody (3A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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