Laminin beta 3 Antibody (CL3353)

Novus Biologicals | Catalog # NBP3-52063

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL3353
View all Laminin beta 3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q13751, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin beta 3 Antibody (CL3353)

Laminin beta 3 Antibody (CL3353) Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Staining of rat rectum shows moderate positivity in basement membrane of glandular cells, as well as strong positivity in endothelial cells.
Laminin beta 3 Antibody (CL3353) Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Staining of rat skin shows moderate to strong positivity in basement membrane basal epidermal cells.
Laminin beta 3 Antibody (CL3353) Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Analysis in human skin and pancreas tissues. Corresponding Laminin beta 3 RNA-seq data are presented for the same tissues.
Laminin beta 3 Antibody (CL3353) Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Staining of human pancreas shows no positivity in glandular cells as expected.
Laminin beta 3 Antibody (CL3353) Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Staining of human skin shows moderate positivity in the basement membrane of the basal layer of epidermal cells.
Laminin beta 3 Antibody (CL3353) Western Blot: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Western Blot: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Analysis in human lung tissue.
Laminin beta 3 Antibody (CL3353) Western Blot: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Western Blot: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.
Laminin beta 3 Antibody (CL3353) Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Staining of rat kidney shows strong positivity in basement membrane in cells in glomeruli.
Laminin beta 3 Antibody (CL3353) Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Staining of human prostate shows moderate to strong positivity in basement membrane of glandular cells.
Laminin beta 3 Antibody (CL3353) Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Staining of rat lung shows moderate to strong positivity in basement membrane of pneumocytes.
Laminin beta 3 Antibody (CL3353) Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Staining of human fallopian tube shows moderate to strong positivity in basement membrane of glandular cells.
Laminin beta 3 Antibody (CL3353) Immunocytochemistry/ Immunofluorescence: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Immunocytochemistry/ Immunofluorescence: Laminin beta 3 Antibody (CL3353) [NBP3-52063]

Staining of A-431 cells, showing specific staining in the endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Applications for Laminin beta 3 Antibody (CL3353)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin beta 3

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.

Alternate Names

BM600-125kDa, Epiligrin subunit bata, FLJ99565, Kalinin B1 chain, Kalinin subunit beta, kalinin-140kDa, LAM5, Laminin 5, Laminin B1k chain, laminin S B3 chain, laminin subunit beta-3, laminin, beta 3, laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD)), Laminin-5 subunit beta, LAMNB1, Nicein subunit beta, nicein-125kDa

Gene Symbol

LAMB3

Additional Laminin beta 3 Products

Product Documents for Laminin beta 3 Antibody (CL3353)

Certificate of Analysis

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Product Specific Notices for Laminin beta 3 Antibody (CL3353)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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