Laminin beta 3 Antibody (CL3353)

Novus Biologicals | Catalog # NBP3-52469

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL3353
View all Laminin beta 3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q13751, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin beta 3 Antibody (CL3353)

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of human fallopian tube shows moderate to strong positivity in basement membrane of glandular cells.
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of human pancreas shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of human prostate shows moderate to strong positivity in basement membrane of glandular cells.
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of human skin shows moderate positivity in the basement membrane of the basal layer of epidermal cells.
Immunocytochemistry/ Immunofluorescence: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunocytochemistry/ Immunofluorescence: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of A-431 cells showing specific staining in the endoplasmic reticulum in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).

Applications for Laminin beta 3 Antibody (CL3353)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laminin beta 3

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.

Alternate Names

BM600-125kDa, Epiligrin subunit bata, FLJ99565, Kalinin B1 chain, Kalinin subunit beta, kalinin-140kDa, LAM5, Laminin 5, Laminin B1k chain, laminin S B3 chain, laminin subunit beta-3, laminin, beta 3, laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD)), Laminin-5 subunit beta, LAMNB1, Nicein subunit beta, nicein-125kDa

Gene Symbol

LAMB3

Additional Laminin beta 3 Products

Product Documents for Laminin beta 3 Antibody (CL3353)

Certificate of Analysis

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Product Specific Notices for Laminin beta 3 Antibody (CL3353)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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