LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-25183

Clone H4A3 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # H4A3

Format

Azide and BSA Free
View all LAMP-1/CD107a Primary Antibodies »

Product Specifications

Immunogen

Human PBMC

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Late Endosome Marker

Specificity

The mouse monoclonal antibody H4A3 recognizes CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

Western Blot: LAMP-1/CD107a Antibody (H4A3)Azide and BSA Free [NBP2-25183]

Western Blot: LAMP-1/CD107a Antibody (H4A3)Azide and BSA Free [NBP2-25183]

Western Blot: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human peripheral blood cells.
Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - HeLa cells.
Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - IHC staining of LAMP-1 [H4A3] catalog number NBP2-25183 in mouse liver. Cytoplasmic and membrane staining was observed in tubule cells and glomeruli.
Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on U-87 cells with LAMP-1/CD107a Antibody (H4A3) NBP2-25183PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.
Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human kidney.
Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on A549 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Surface staining of human peripheral blood cells with anti-CD107a (H4A3) PE.
Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on THP-1 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) azide free, GAM-APC.
Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) APC.
Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) Alexa Fluor (R) 488.
Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Analysis of PHA-activated PBMC with anti-CD107a (H4A3) PerCP-CyTM5.5.
Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on A431 cells with LAMP-1/CD107a Antibody [H4A3] NBP2-25183AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free [NBP2-25183]

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on U-87 cells with LAMP-1/CD107a Antibody (H4A3) NBP2-25183AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

LAMP-1/CD107a (H4A3) in A431 Human Cell Line.

LAMP-1/CD107a (H4A3) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti- LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-25183AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

LAMP-1/CD107a (H4A3) in A431 Human Cell Line.

LAMP-1/CD107a (H4A3) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti- LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP2-25183R) (red) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

LAMP-1/CD107a (H4A3) in U-2 OS Human Cell Line.

LAMP-1/CD107a (H4A3) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP2-25183AF488) (green) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

Detection of LAMP-1/CD107a (H4A3) in U-2 OS Human Cell Line by Flow Cytometry.

An intracellular stain was performed on U-2 OS human osteosarcoma cell line with Mouse anti-LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP2-25183AF488, blue histogram) or matched control antibody (orange histogram) at 5 µg/mL for 30 minutes at RT.
LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

Detection of LAMP-1/CD107a (H4A3) in A431 Human Cell Line by Flow Cytometry.

An intracellular stain was performed on A431 human skin carcinoma cell line using Mouse anti-LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-25183AF647, blue histogram) or matched control antibody (orange histogram) at 2.5 µg/mL for 30 minutes at RT.
LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

Detection of LAMP-1/CD107a (H4A3) in A431 Human Cell Line by Flow Cytometry.

An intracellular stain was performed on A431 human skin carcinoma cell line using Mouse anti-LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 700 (Catalog # NBP2-25183AF700, blue histogram) or matched control antibody (orange histogram) at 2.5 µg/mL for 30 minutes at RT.
LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

Detection of LAMP-1/CD107a (H4A3) in A431 Human Cell Line by Flow Cytometry.

An intracellular stain was performed on A431 human skin carcinoma cell line using Mouse anti-LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP2-25183R, blue histogram) or matched control antibody (orange histogram) at 2.5 µg/mL for 30 minutes at RT.
LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

LAMP-1/CD107a (H4A3) in HepG2 Human Cell Line.

LAMP-1/CD107a (H4A3) was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse anti-LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-25183AF647) (light blue) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

LAMP-1/CD107a (H4A3) in NIH-3T3 Mouse Cell Line.

LAMP-1/CD107a (H4A3) was detected in immersion fixed NIH-3T3 Mouse fibroblast cell line using Mouse anti-LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-25183AF647) (light blue) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1;10-1;2000

Immunohistochemistry

1;10-1;20000

Immunohistochemistry-Frozen

1:10-1:5000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1 - 2 ug/ml
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.00 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LAMP-1/CD107a

LAMP-1 (lysosome-associated membrane protein 1), also known as CD107a (cluster of differentiation 107a), is a major component of lysosomal membranes that plays an important role in lysosomal biogenesis, autophagy, and cholesterol metabolism (1). LAMP-1 is a type I transmembrane glycoprotein that is expressed on plasma membranes and the membranes of endosomes, autolysosomes, and lysosomes (1,2). Additionally, LAMP-1/CD107a is a commonly used marker for natural killer (NK) cell degranulation (3). LAMP-1 and another lysosomal-associated membrane protein, LAMP-2, together make up about half of all lysosome membrane proteins (1). Additionally, LAMP-1 has a role in presenting carbohydrate ligands to selectins (2). Human LAMP-1 protein is comprised of 417 amino acids (aa) with a theoretical molecular weight of 44.8 kDa; however, glycosylation can increase the molecular weight upwards of 120 kDa (1, 4). Structurally, LAMP-1 protein contains a large luminal/extracellular domain (29-382 aa), a helical transmembrane domain (383-405 aa), and a short cytoplasmic tail (406-417 aa) (1,2). Additionally, the protein has many N- and O-linked glycosylation sites which helps with stability in the membrane (1,2).

LAMP-1 plays an important role in autophagy-mediated ATP-release during apoptosis where lysosomes containing intracellular ATP migrate to the plasma membrane and, during exocytosis, LAMP-1 is exposed to the cell surface (5). Studies have found that knockdown of LAMP-1 blocks the ATP release from the cell (5). Furthermore, an absence of LAMP-1 and LAMP-2 leads to an accumulation of lysosomal cholesterol (6). Lysosomal membrane dysfunction or defects has also been associated with disease development (6,7). For example, one feature of pancreatitis is autophagy impairment which is caused by lysosomal dysfunction and a corresponding decrease in lysosomal-membrane associated proteins LAMP-1 and LAMP-2 (7).

References

1. Eskelinen E. L. (2006). Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Molecular aspects of medicine, 27(5-6), 495-502. https://doi.org/10.1016/j.mam.2006.08.005

2. Cheng, X. T., Xie, Y. X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z. H. (2018). Revisiting LAMP1 as a marker for degradative autophagy-lysosomal organelles in the nervous system. Autophagy, 14(8), 1472-1474. https://doi.org/10.1080/15548627.2018.1482147

3. Krzewski, K., & Coligan, J. E. (2012). Human NK cell lytic granules and regulation of their exocytosis. Frontiers in immunology, 3, 335. https://doi.org/10.3389/fimmu.2012.00335

4. Uniprot (P11279)

5. Wang, Y., Martins, I., Ma, Y., Kepp, O., Galluzzi, L., & Kroemer, G. (2013). Autophagy-dependent ATP release from dying cells via lysosomal exocytosis. Autophagy, 9(10), 1624-1625. https://doi.org/10.4161/auto.25873

6. Schwake, M., Schr0der, B., & Saftig, P. (2013). Lysosomal membrane proteins and their central role in physiology. Traffic (Copenhagen, Denmark), 14(7), 739-748. https://doi.org/10.1111/tra.12056

7. Gukovsky, I., Pandol, S. J., Mareninova, O. A., Shalbueva, N., Jia, W., & Gukovskaya, A. S. (2012). Impaired autophagy and organellar dysfunction in pancreatitis. Journal of gastroenterology and hepatology, 27 Suppl 2(Suppl 2), 27-32. https://doi.org/10.1111/j.1440-1746.2011.07004.x

Long Name

Lysosome-associated Membrane Glycoprotein 1

Alternate Names

CD107a, LAMP1

Gene Symbol

LAMP1

Additional LAMP-1/CD107a Products

Product Documents for LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

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Product Specific Notices for LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

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